A5011841078- Malaria Research and Control
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Vector-borne infectious diseases
- Evolution and Genetic Dynamics
- Travel-related health issues
- Parasites and Host Interactions
- Global Maternal and Child Health
- Migration, Identity, and Health
- Drug-Induced Hepatotoxicity and Protection
- Zoonotic diseases and public health
- Animal Virus Infections Studies
- COVID-19 epidemiological studies
- Influenza Virus Research Studies
- Drug Transport and Resistance Mechanisms
- Hemoglobinopathies and Related Disorders
- Computational Drug Discovery Methods
- Inflammatory Myopathies and Dermatomyositis
- Heparin-Induced Thrombocytopenia and Thrombosis
- Pregnancy and Medication Impact
- Traditional and Medicinal Uses of Annonaceae
- Eosinophilic Disorders and Syndromes
- Blood donation and transfusion practices
Institut Pasteur
2015-2025
Institut Pasteur de la Guyane
2015-2025
Sorbonne Paris Cité
2025
Université Paris Cité
2022-2025
Centre d'Immunologie et des Maladies Infectieuses
2021-2023
Université de Perpignan
2021
Laboratoire Génome et Développement des Plantes
2021
Université de Bordeaux
2021
UMR BIOdiversity, GEnes & Communities
2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021
Significance This study addresses the evolutionary dynamics of antimalarial drug resistance after changes in use. We show that chloroquine Plasmodium falciparum from French Guiana was lost sustained removal, whereas marker PfCRT K76T remained fixed parasite population. phenotypic reversion caused by acquisition a single additional C350R substitution PfCRT. genetic change also impaired susceptibility to piperaquine, suggesting piperaquine pressure drove expansion this allele. These findings...
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces an antibody response targeting multiple antigens that changes over time. This study aims to take advantage of this complexity develop more accurate serological diagnostics.A multiplex assay was developed measure IgG and IgM responses seven SARS-CoV-2 spike or nucleoprotein antigens, two for the nucleoproteins 229E NL63 seasonal coronaviruses, three non-coronavirus antigens. Antibodies were measured in serum...
Malaria is endemic in French Guiana, an overseas territory of France on the Guiana Shield. Since 2005, notified malaria cases are decreasing. However, new data show that affects many Brazilian gold miners working illegally majority whom not counted official data. In addition, one major concern usual practice improper self-treatment this mining population, raising fear development anti-malarial resistance. This prospective study, conducted 2015, aimed to estimate prevalence Plasmodium spp....
Many SARS-CoV-2-infected individuals remain asymptomatic. Little is known about the extent and quality of their antiviral humoral response. Here, we analyze antibody functions in 52 asymptomatic infected individuals, 119 mildly symptomatic, 21 hospitalized patients with COVID-19. We measure anti-spike immunoglobulin G (IgG), IgA, IgM levels S-Flow assay map IgG-targeted epitopes a Luminex assay. also evaluate neutralization, complement deposition, antibody-dependent cellular cytotoxicity...
BackgroundChildren are underrepresented in the COVID-19 pandemic and often experience milder disease than adolescents adults. Reduced severity is possibly due to recent more frequent seasonal human coronaviruses (HCoV) infections. We assessed seroprevalence of SARS-CoV-2 HCoV specific antibodies a large cohort north-eastern France.MethodsIn this cross-sectional study, serum samples were collected from children adults requiring hospital admission for non-COVID-19 between February August 2020....
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces a complex antibody response that varies by orders of magnitude between individuals and over time.
Plasmodium falciparum is an apicomplexan parasite responsible for lethal cases of malaria. According to WHO recommendations, P are treated with artemisinin-based combination therapy including dihydroartemisinin-piperaquine. However, the emergence resistant parasites against dihydroartemisinin-piperaquine was reported in southeast Asia 2008 and, a few years later, suspected South America.
ABSTRACT Polymorphisms in the Plasmodium falciparum crt (Pf ), Pf mdr1 , and mrp genes were not significantly associated with quinine (QN) 50% inhibitory concentrations (IC 50 s) 23 strains of . An increased number DNNND repeats nhe-1 microsatellite ms4760 was an IC QN ( P = 0.0007). Strains only one repeat more susceptible to (mean 154 nM). two had intermediate susceptibility 548 three reduced 764 Increased numbers NHNDNHNNDDD a decreased 0.0020). profile 7 for (ms4760-7) The determination...
Molecular detection methods have revealed higher sensitivity and specificity than conventional microscopy or rapid diagnostic tests for malaria diagnosis. In this study, we implemented, evaluated validated according to the ISO 15,189 requirements, a multiplex real-time PCR assay detect identify five human parasites. DNA samples were extracted from whole blood dried spots drawn patients. Based on External Quality Assessment (whole blood), method shows 100% specificity. This detected P. vivax...
Malaria is endemic in French Guiana (FG), South America. Despite the decrease cases local population, illegal gold miners are very affected by malaria (22.3% of them carried Plasmodium spp.). Self-medication seems to be common, but its modalities and associated factors have not been studied. The aim this study was evaluate parasite susceptibility drugs document behaviours that could contribute resistance selection miners.This multicentric cross-sectional conducted resting sites along...
To assess the prevalence of malaria among illegal gold miners in French Guiana rainforest, we screened 205 during May-June 2014. Malaria was 48.3%; 48.5% cases were asymptomatic. Patients reported self-medication with artemisinin-based combination therapy. Risk for emergence and spread artemisinin resistance rainforest is high.
Abstract Background Infection with SARS-CoV-2 induces an antibody response targeting multiple antigens that changes over time. This complexity presents challenges and opportunities for serological diagnostics. Methods A multiplex assay was developed to measure IgG IgM responses seven spike or nucleoprotein antigens, two the nucleoproteins of 229E NL63 seasonal coronaviruses, three non-coronavirus antigens. Antibodies were measured in serum samples from patients French hospitals RT-qPCR...
Serological assays capable of measuring antibody responses induced by previous infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been critical tools in the response to COVID-19 pandemic. In this study, we use bead-based multiplex measure IgG and IgA antibodies avidity five SARS-CoV-2 antigens (Spike (S), receptor-binding domain (RBD), Nucleocapsid (N), S subunit 2, Membrane-Envelope fusion (ME)). These were performed several cohorts healthcare workers nursing...
To implement future malaria elimination strategies in French Guiana, a characterization of the infectious reservoir is recommended. A cross-sectional survey was conducted between October and December 2017 Guianese municipality St Georges de l’Oyapock, located along Brazilian border. The prevalence Plasmodium spp . determined using rapid diagnostic test (RDT) polymerase chain reaction (PCR). Demographic, house locations, medical history, biological data were analyzed. Factors associated with...
Background The risk of SARS-CoV-2 (re-)infection remains present given waning vaccine-induced and infection-acquired immunity, ongoing circulation new variants. Aim To develop a method that predicts virus neutralisation disease protection based on variant-specific antibody measurements to antigens. Methods correlate titres, we collected 304 serum samples from individuals with either or immunity. Using the association between developed prediction model for SARS-CoV-2-specific titres. From...
Establishing correlates of protection often requires large cohorts. A rapid and adaptable case-control study design can be used to identify antibody against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in serum saliva. We designed a compare levels between cases SARS-CoV-2 within 5 days symptom onset uninfected controls. Controls were matched on age, number disease 2019 vaccine doses, time since last dose, past episodes infection. quantified anti-SARS-CoV-2 seasonal...
The malaria parasite Plasmodium falciparum regularly confronts orchestrated changes in frontline drug treatment that drastically alter its selection landscape. When this has occurred, the successfully adapted to new drugs through novel resistance mutations. These mutations, however, emerge a genetic background already shaped by prior selection. In some instances, imposed different target same loci either synergistic or antagonistic ways, which may leave genomic signatures are hard attribute...
Numerous studies elucidated the kinetics of humoral immune response post-SARS-CoV-2 infection. However, in sub-Saharan Africa, evolution SARS-CoV-2 IgG antibody responses and their interaction with pre-existing seasonal human coronavirus (HCoVs: OC43, 229E, NL63, HKU1) immunity remain underexplored. A prospective cohort study was conducted Senegal during first year COVID-19 pandemic (March to December 2020). total 204 patients laboratory-confirmed were included. Patients classified as...
Recent studies suggest shared pathogenic pathways during malaria and allergy. Indeed, IgE, histamine, the parasite-derived Plasmodium falciparum histamine-releasing factor translationally controlled tumor protein (PfTCTP) can be found at high levels in serum from patients experiencing malaria, but their relationship with basophil activation remains unknown. We recruited P. falciparum-infected Senegal mild (MM; n = 19) or severe (SM; 9) symptoms healthy controls (HC; 38). Levels of PfTCTP,...
Abstract A large proportion of SARS-CoV-2 infected individuals remains asymptomatic. Little is known about the extent and quality their antiviral humoral response. Here, we analyzed antibody functions in 52 asymptomatic individuals, 119 mild 21 hospitalized COVID-19 patients. We measured anti-Spike levels with S-Flow assay mapped Spike- N-targeted regions by Luminex. Neutralization, complement deposition Antibody-Dependent Cellular Cytotoxicity (ADCC) were evaluated using...