A5089304086- T-cell and Retrovirus Studies
- Malaria Research and Control
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Animal Disease Management and Epidemiology
- COVID-19 epidemiological studies
- Vector-Borne Animal Diseases
- Complement system in diseases
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- RNA regulation and disease
- Poverty, Education, and Child Welfare
- Global Maternal and Child Health
- Reproductive System and Pregnancy
- Primate Behavior and Ecology
- Biosensors and Analytical Detection
- Eosinophilic Disorders and Syndromes
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Animal Virus Infections Studies
- Travel-related health issues
- Respiratory viral infections research
- Parasites and Host Interactions
- Reproductive Physiology in Livestock
Université Paris Cité
2022-2024
Mère et Enfant en Milieu Tropical
2024
Institut de Recherche pour le Développement
2024
Institut Pasteur
2020-2022
Sorbonne Université
2020-2022
Université Paris 1 Panthéon-Sorbonne
2021
Institut Pierre Louis d‘Épidémiologie et de Santé Publique
2021
Université Sorbonne Nouvelle
2021
Institut thématique Santé Publique
2020
Santé Publique France
2020
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces an antibody response targeting multiple antigens that changes over time. This study aims to take advantage of this complexity develop more accurate serological diagnostics.A multiplex assay was developed measure IgG and IgM responses seven SARS-CoV-2 spike or nucleoprotein antigens, two for the nucleoproteins 229E NL63 seasonal coronaviruses, three non-coronavirus antigens. Antibodies were measured in serum...
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces a complex antibody response that varies by orders of magnitude between individuals and over time.
Abstract Background Infection with SARS-CoV-2 induces an antibody response targeting multiple antigens that changes over time. This complexity presents challenges and opportunities for serological diagnostics. Methods A multiplex assay was developed to measure IgG IgM responses seven spike or nucleoprotein antigens, two the nucleoproteins of 229E NL63 seasonal coronaviruses, three non-coronavirus antigens. Antibodies were measured in serum samples from patients French hospitals RT-qPCR...
Serological assays capable of measuring antibody responses induced by previous infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been critical tools in the response to COVID-19 pandemic. In this study, we use bead-based multiplex measure IgG and IgA antibodies avidity five SARS-CoV-2 antigens (Spike (S), receptor-binding domain (RBD), Nucleocapsid (N), S subunit 2, Membrane-Envelope fusion (ME)). These were performed several cohorts healthcare workers nursing...
Background Antibody responses as serological markers of Plasmodium vivax infection have been shown to correlate with exposure, but little is known about the other factors that affect antibody in naturally infected people from endemic settings. To address this question, we studied IgG novel exposure (SEMs) P . three settings different transmission intensity. Methodology We validated a panel 34 SEMs Peruvian cohort up years’ longitudinal follow-up using multiplex platform and compared results...
Background The landscape of malaria transmission in the Peruvian Amazon is temporally and spatially heterogeneous, presenting different micro-geographies with particular epidemiologies. Most cases are asymptomatic escape routine surveillance based on light microscopy (LM). Following implementation control programs this region, new approaches to stratify direct efforts at an individual community level needed. Antibody responses serological exposure markers (SEM) Plasmodium vivax have proven...
Plasmodium vivax infections are characterised by recurrent bouts of blood-stage parasitemia. Understanding the genetic relatedness recurrences can distinguish whether these caused relapse, reinfection, or recrudescence, which is critical to understand treatment efficacy and transmission dynamics. We developed PvAmpseq, an amplicon sequencing assay targeting 11 SNP-rich regions P. genome. PvAmpSeq was validated on field isolates from a clinical trial in Solomon Islands longitudinal...
Abstract Malaria risk is highly heterogeneous. Understanding village and household-level spatial heterogeneity of malaria can support a transition to spatially targeted interventions for elimination. This analysis uses data from cross-sectional prevalence surveys conducted in 2014 2016 two villages (Megiar Mirap) Papua New Guinea. Generalised additive modelling was used characterise investigate the contribution individual, household environmental-level factors. Following period declining...
The measurement of recent malaria exposure can support control efforts. This study evaluated serological responses to an in-house Plasmodium vivax Merozoite Surface Protein 8 (PvMSP8) expressed in a Baculovirus system as sero-marker P. (Pv) the Peruvian Amazon. In first evaluation, IgGs against PvMSP8 and PvMSP10 proteins were measured by Luminex cohort 422 Amazonian individuals with known history Pv (monthly data infection status qPCR and/or microscopy over five months). Both able...
The epidemiology of malaria changes as prevalence falls in low-transmission settings, with remaining infections becoming more difficult to detect and diagnose. At this stage active surveillance is critical residual hotspots transmission. However, diagnostic tools used generally only concurrent infections, surveys may benefit from sensitive such serological assays. Serology can be interrogate characterize individuals' previous exposure over longer durations, providing information essential...
Abstract Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces a complex antibody response that varies by orders of magnitude between individuals and over time. Waning levels lead to reduced sensitivity serological diagnostic tests This undermines the utility surveillance as SARS-CoV-2 pandemic progresses into its second year. Here we develop multiplex test for measuring antibodies three isotypes (IgG, IgM, IgA) five antigens (Spike (S), receptor binding domain...
Human T‐lymphotropic virus 1 (HTLV‐1) is the etiologic agent of HTLV‐1‐associated myelopathy/tropical spastic paraparesis (HAM/TSP). Apoptosis a mechanism defense elicited by many triggers, including cross‐linking FAS receptor expressed in viruses‐infected cells, and ligand FASL presented T‐cytotoxic cells. As HAM/TSP has been associated with high levels proviral load (PVL), we hypothesized that certain genotypes single‐nucleotide polymorphisms (SNPs) decreased protein expression could be...
A more sensitive surveillance tool is needed to identify Plasmodium vivax infections for treatment and accelerate malaria elimination efforts. To address this challenge, our laboratory has developed an eight-antigen panel that detects total IgG as serological markers of P. exposure within the prior 9 months. The value these been established use in areas with low transmission. In moderate–high transmission areas, there evidence long-lived than transmission, resulting poorer performance...
To assess the hypothesis that HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) runs in families, we compared frequency of HAM/ TSP among HTLV-1-positive relatives HAM/TSP patients with HTLV1-positive asymptomatic HTLV-1 carriers. We reviewed available information at Instituto de Medicina Tropical Alexander von Humboldt Lima (period 1990-2012). Index cases were defined as unrelated, a clinical diagnosis for whom was motive testing and who brought ≥1 relative (blood and/or...
In the Amazon Region of Peru, occupational activities are important drivers human mobility and may increase individual risk being infected while contributing to increasing malaria community-level transmission. Even though out-of-village working other patterns have been identified as determinants transmission, no studies quantified effect on recent exposure proposed plausible intervention scenarios. Using two population-based cross-sectional in Loreto Department parametric g-formula method,...
Given the altered responses to both artemisinins and lumefantrine in Eastern Africa, monitoring antimalarial drug resistance all African countries is paramount. We measured susceptibility six antimalarials using ex vivo growth inhibition assays (IC50) for a total of 805 Plasmodium falciparum isolates obtained from travelers returning France (2016-2023), mainly West Central Africa. Isolates were sequenced molecular inversion probes (MIPs) targeting fourteen genes across parasite genome. Ex...
Given the altered responses to both artemisinins and lumefantrine in Eastern Africa, monitoring antimalarial drug resistance all African countries is paramount.
Abstract SARS-CoV-2 remains a significant health threat due to its high infection and mutation rates. The emergence of new variants concern poses challenges as they can lead immune escape mutations, potentially reducing the efficacy vaccines antibody therapeutics. receptor binding domain (RBD) is particularly noteworthy it both most rapidly evolving principal target neutralizing antibodies. As an alternative time-consuming expensive neutralization assays, we have developed bead-based...
MicroRNAs (miRNAs) are small non-coding RNAs that regulate protein expression. HTLV-1 is able to promote oncogenesis in T cells by altering the expression of miRNAs involved control cell-cycle. It not known whether deregulates HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients. To asses if infection might alter inflammatory response, we evaluated 84 process asymptomatic carriers (AC) and HAM/TSP patients using miScript miRNA PCR Array Human Inflammatory Response &...
Human T-cell lymphotropic Virus Type I (HTLV-1) is a retrovirus that generates an inflammatory response can trigger neurological disease called HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) which appears in 5-10% of infected people around the world. This process due action anti-Tax CD8+ lymphocytes employ cytokines order to stop predominant infiltration CD4+ T lymphocytes. IL8 pro-inflammatory cytokine induces activation and migration neutrophils towards sites infection...
HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a neuroinflammatory, non-remitting and disabling disease.NF-β pathway plays role in the pathogenesis of this condition.We compare expression at peripheral level of84 genes involved NF-β among asymptomatic HTLV-1 carriers (AC) HAM/ TSP patients.mRNA from PBMCs was isolated 12 classified into three groups: fourAC (=patients without any neurological condition) eight patients with HAM/TSP (four EDSS scores 1-5 four score...
High proviral loads have been described in HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients comparison to asymptomatic HTLV-1 carriers (AC). However, biomarkers related HAM/TSP progression not identified. We analyzed differential proteome changes by two-dimensional gel electrophoresis (2D-electrophoresis) identify spots of proteins plasma samples three groups patients: five AC, nine with EDSS scores 1.0-5.0 (=mild HAM/TSP) and 4 5.5-9.0 (=severe HAM/TSP). Proteins...
Background NKG2D is an activating receptor mainly expressed on Natural Killer (NK) cells, its ligands has abnormal expression in virus infected cells and tumoral cells. The SNP rs2255336 G/A generates a substitution of alanine to threonine (NKG2D Thr72Ala) associated with low high cytotoxic activity NK respectively. This study investigated the association between Thr72Ala polymorphism HAM/TSP PVL values Peruvian HTLV-1 patients asymptomatic carriers.
Adult T-cell leukemia/lymphoma (ATLL) is an aggressive neoplastic disease whose etiologic agent HTLV-1. ATLL patients have a pronounced immunosuppression of innate and adaptive immune system, compared to asymptomatic carriers. Killer-cell immunoglobulin-like receptors (KIR) play important role in the signaling natural killer cells also are involved activity CD8+ T cells, therefore these great repercussion response. According their intracytoplasmic motif they activating or inhibiting function...