A5015118073- Cancer, Hypoxia, and Metabolism
- Multiple Myeloma Research and Treatments
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Malaria Research and Control
- Lipid Membrane Structure and Behavior
- Metabolomics and Mass Spectrometry Studies
- Cancer-related molecular mechanisms research
- Cancer Research and Treatments
- Scientific Measurement and Uncertainty Evaluation
- Circular RNAs in diseases
- Philosophy, Science, and History
- Amino Acid Enzymes and Metabolism
- Epigenetics and DNA Methylation
- Healthcare, Law, Governance, and Management Studies
- Inhalation and Respiratory Drug Delivery
- Porphyrin Metabolism and Disorders
- Biocrusts and Microbial Ecology
- Cancer Treatment and Pharmacology
- Phenomenology and Existential Philosophy
- Biochemical Acid Research Studies
- Angiogenesis and VEGF in Cancer
- Biomedical and Engineering Education
- Medical History and Research
- MicroRNA in disease regulation
Jagiellonian University
2015-2025
Massachusetts Institute of Technology
2024-2025
University Hospital Kralovske Vinohrady
1995
The cell NAD+/NADH ratio can constrain biomass synthesis and influence proliferation in nutrient-limited environments. However, which processes regulate the is not known. Here, we find that some cancer cells elevate response to serine deprivation by increasing mitochondrial respiration. Cancer respiration have higher production limiting conditions than with no response, independent of enzyme expression. Increases promote regardless whether environmentally limiting. Lipid also increase via...
Abstract Background Multiple myeloma (MM) is defined as plasma cells malignancy, developing in the bone marrow. At beginning of disease, malignant are dependent on marrow microenvironment, providing growth and survival factors. Importantly, recent studies pointed hypoxia an important factor promoting progression MM. In particular, hypoxia-triggered HIF-1 signaling was shown to promote chemoresistance, angiogenesis, invasiveness induction immature phenotype, suggesting that strategies...
Abstract By reducing the surface tension of air–water interface in alveoli, lung surfactant (LS) is crucial for proper functioning lungs. It also forms first barrier against inhaled pathogens. In this study we inspect interactions LS models with a dangerous air pollutant, benzo[a]pyrene (BaP). Dipalmitoylphosphatidylcholine (DPPC), 1‐palmitoyl‐2‐oleoylphosphatidylcholine, and their 1:1 mixture are used as models. Pressure‐area isotherms employed to macroscopic properties monolayers. We find...
Most melanomas develop in hypoxic conditions. Since hypoxia via HIF-1 induces glycolysis, a process essential for malignant melanoma growth/survival, the goal of this study was to analyze influence on expression target genes involved glucose metabolism.The response cell lines conditions analyzed by RT-PCR and western blotting. A Kaplan-Meier survival analysis patients with high low level PFKFB4 performed. Further patients' data performed using R/Bioconductor environment.Induction gene can be...
The interaction between five gemini amphiphilic pseudopeptides (GAPs) differing by the length of central spacer and a model membrane lipid, 1,3-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol (cardiolipin) were studied with aim to evaluate their possible antimicrobial properties. To this end, monomolecular films formed at air/water interface pure cardiolipin or cardiolipin/GAPs mixtures; film properties determined using surface pressure potential measurements, as well...
Multiple myeloma (MM) is characterized as a clonal expansion of malignant plasma cells in the bone marrow, what often associated with pancytopenia and osteolytic disease. Interestingly, myeloma-infiltrated marrow considered to be hypoxic, providing selection pressure for developing tumour. Since HSP90 was shown participate stabilization subunit key transcription factor HIF-1, which controls hypoxic response, aim this study investigate influence inhibitor...
During cancer progression metabolic reprogramming is observed in parallel to the alternation transcriptional profiles of malignant cells. Recent studies suggest that isoenzymes phosphofructokinase II (PFK-II) - PFKFB3 and PFKFB4, often induced hypoxic environment, significantly contribute enhancement glucose metabolism consequence progression.Using publicly available data deposited R2 base we performed a Kaplan-Meyer analysis for patients divided into groups with high low expression levels...
Multiple myeloma (MM) is characterized as a clonal expansion of malignant plasma cells in the bone marrow, which often associated with pancytopenia and osteolytic disease. Interestingly, myeloma-infiltrated marrow considered to be hypoxic, providing selection pressure for developing tumour. Since HSP90 was shown participate stabilization subunit key transcription factor HIF-1, controls hypoxic response, aim this study investigate influence inhibitor 17-allylamino-17-demethoxygeldanamycin...
The CD146 (also known as MCAM, MUC-18, Mel-CAM) was initially reported in 1987, a protein crucial for the invasiveness of malignant melanoma. Recently, it has been confirmed that involved progression and poor overall survival many cancers including breast cancer. Importantly, independent studies, to be trigger epithelial mesenchymal transition cancer cells. goal our current study verify potential involvement epigenetic mechanism behind regulation expression cells, previously prostate First,...
During cancer progression cells undergo epithelial-to-mesenchymal transition (EMT). Although EMT is a complex process, recently, it has been reported that CD146 overexpression in prostate sufficient to induce mesenchymal phenotype. The following study aimed investigate whether the expression of altered by an epigenetic modifier cells, vitro.Three human cell lines were treated with 5-aza-2-deoxycytidine; and EMT-related factors was analyzed RT-PCR western Blot. methylation status promoter...
Inverted internal limiting membrane (ILM) flap may constitute a scaffold for Muller cells whose migration and proliferation on its surface begin the process of macular hole closure. The goal study was to establish an in vitro model interaction between ILM cells. Vitrectomy with inverted performed 23 patients due full-thickness (FTMH). After dissection flap, area peeling extended material collected cell culture experiments. cultured adherent plates showed significantly better growth than...
Abstract Coordination of adaptive metabolism through cellular signaling networks and metabolic response is essential for balanced flow energy homeostasis. Post-translational modifications such as phosphorylation offer a rapid, efficient, dynamic mechanism to regulate networks. Although numerous sites have been identified on enzymes, much remains unknown about their contribution enzyme function systemic metabolism. In this study, we stratify enzymes based location with respect functional...
The 23rd FEBS YSF was held from 26th to 29th June 2024 in Pavia, Italy. Over 100 PhD students and early postdoctoral researchers around 30 different countries came together at the inspiring rooms of University Pavia for a four‐day event. This year's topic ‘Biochemistry bridging gap’, meaning opportunity have comprehensive perspective on all biochemistry applications. Four renowned keynote speakers presented their latest research, accompanied by four career‐focused speakers, as well...
Cancer-specific isoenzyme of phosphofructokinase II (PFKFB4), as our previous research has shown, may be one the most important enzymes contributing to intensification glycolysis in hypoxic malignant melanoma cells. Although PFKFB4 gene seems play a crucial role progression melanoma, so far there are no complete data on expression at isoform level and influence hypoxia alternative splicing. Using RT-qPCR semi-quantitative RT-PCR, we presented profile six isoforms described OMIM NCBI database...
Abstract Background/aim: Reduced partial oxygen pressure is a characteristic feature of many cancers. HIF-1 transcription factor, activated under hypoxic conditions, alters the gene expression profile, triggering genes, which facilitate survival cells in diminished environment. Importantly, signaling pathway itself has been considered potential target anti-cancer therapy since inhibition this may significantly slow down tumor growth. Materials and methods Initially, using vitro conditions we...