Abstract Aims We evaluated the safety, feasibility and initial effects of therapy with muscle‐derived cells (MDCs) for women stress urinary incontinence (SUI). Methods MDCs were isolated from an upper‐arm muscle biopsy 16 SUI. Cells by enzymatic digestion expanded in vitro 8–10 weeks. A quantity 0.6–25 × 10 6 obtained injected transurethrally into urethral rhabdosphincter under local anesthesia. The placed circumferentially at 9, 12, 3 O'clock positions endoscopic guidance. Results results...

The type of diet not only affects the composition oral microflora but is also one more critical factors associated with an increased risk Parkinson's disease, PD. This study compared preferences and microbiota profiles in patients PD vs. healthy controls. 59 108 controls (without neurodegeneration) using 16S rRNA gene amplicon sequencing. According to results, different from In particular, decreased abundance Proteobacteria, Pastescibacteria, Tenercutes was observed. cavity characterized by...

The aim of this study was to investigate the role urine-derived extracellular vesicles (uEVs) in diabetic kidney disease (DKD) patients diagnosed with type 2 diabetes mellitus (T2DM).UEVs were characterized by size distribution and microRNA content next-generation small RNA sequencing quantitative reverse transcription PCR.A subset sixteen miRNAs enriched T2DM DKD, including hsa-miR-514a-5p, hsa-miR‑451a, hsa-miR-126-3p, hsa-miR-214, or hsa-miR‑503 identified. Eight as hsa-miR-21-3p,...

The presented analysis concerns the inter-domain and inter-protein interface in protein complexes. We propose extending traditional understanding of domain as a function local compactness with an additional criterion which refers to presence well-defined hydrophobic core. Interface areas selected homodimers vary respect their contribution share well individual (domain-specific) cores. basic definition domain, i.e., structural unit characterized by tighter packing than its immediate...

One of the critical steps in molecular oncology diagnostics is obtaining high quality genomic DNA. Therefore, it important to evaluate and compare techniques used extract DNA from tissue samples. Since formalin-fixed, paraffin-embedded (FFPE) tissues are routinely for both retrospective prospective studies, we compared three commercially available methods nucleic acid extraction terms quantity isolated DNA.Slides prepared 42 FFPE blocks were macro-dissected. Resulting material was divided...

The aim of the study was to evaluate frequency breakfast and other meal consumption by adolescents assess relationship between first last sex, body mass index (BMI), middle school high students' education level. conducted in 2013-2014 among 3009 students (1658 girls 1351 boys) from s schools Krakow Silesia (Poland). data obtained questionnaires that were analyzed with a logistic regression model for measurable dichotomous variables. Breakfast consumers seen eat meals (second breakfast,...

Abstract Background Hyperandrogenism is among the most common endocrine disorders in women. Clinically, it manifests as hirsutism, acne, and alopecia. A healthy lifestyle, including nutritious dietary patterns physical activity, may influence clinical manifestation of disease. This study determined effect a low-glycemic index anti-inflammatory diet on testosterone levels sex hormone-binding globulin (SHBG) symptoms hyperandrogenic women at their reproductive age. Methods The included 44...

Introduction:Regenerative medicine for the treatment of urinary incontinence has become a popular area focus in search therapies this disease.The paper focused on women' s quality life assessment who were subjected to transplantation MDSC (autologous muscle derived stem cells) urethral sphincter. Methods:The procedure was conducted 16 female patients completed observation stage.Assessment before and after (two four years post-operation) based validated I-QOL questionnaire (the Polish...

(1) Background: Static foot deformities, including hallux valgus, are common deformities. The subject under consideration is the role of extrinsic and intrinsic muscles working within muscular systems that shape arches alignment toes. (2) Methods: Based on a literature review, muscle were analyzed. as follows: “tendon stirrup” (system I); complementary to tendon stirrup II); “foot lever” III); system III IV); “reins hallux” V), having distal inserts hallux. was analyzed in this context. (3)...

The study of interactions between molecules belonging to different biochemical families (such as lipids and nucleic acids) requires specialized data analysis methods. This article describes the DNA Microarray Integromics Analysis Platform, a unique web application that focuses on computational integration "multi-omics" data. Our tool supports range complex analyses, including – among others low- high-level analyses microarray data, integrated transcriptomics lipidomics ability infer...

Most melanomas develop in hypoxic conditions. Since hypoxia via HIF-1 induces glycolysis, a process essential for malignant melanoma growth/survival, the goal of this study was to analyze influence on expression target genes involved glucose metabolism.The response cell lines conditions analyzed by RT-PCR and western blotting. A Kaplan-Meier survival analysis patients with high low level PFKFB4 performed. Further patients' data performed using R/Bioconductor environment.Induction gene can be...

Objectives: Exosomes — microvesicles which are secreted by living cells can be produced from different cell types and detected in various body fluids. They the carriers of intercellular information regulate tumor microenvironment considered to involved progression metastasis. Cancer secrete more exosomes than healthy cells, expected potential tools for diagnosis treatment. Material methods: In this report, we present results microparticle analysis peripheral uterine blood patients with...

Abstract The three‐dimensional structures of a set ‘never born proteins’ (NBP, random amino acid sequence proteins with no significant homology known proteins) were predicted using two methods: Rosetta and the one based on ‘fuzzy‐oil‐drop’ (FOD) model. More than 3000 different sequences have been generated, filtered against non redundant protein data base, to remove proteins, subjected structure prediction. Comparison between FOD predictions allowed select ten top (highest structural...

A huge amount of atomized biological data collected in various databases and the need for a description their relation by theoretical methods causes development integration methods. The omics analysis knowledge with mathematical procedures implemented OmicsON R library is presented paper. tool two sets data: transcriptomics metabolomics. In workflow library, functional grouping statistical are applied. Subgroups among transcriptomic metabolomics created based on stored Reactome String...

(1) Background: The purpose of this study was to observe segmental phase angle (PhA) and body composition fluctuation elite ski jumpers. (2) Methods: In the study, 12 professional jumpers took part. Body estimated with multi-frequency bioelectrical impedance analysis. Repeated ANOVA used check parameters’ variability in time. symmetry between right left side verified t-test for dependent samples. Pearson’s linear correlation coefficient calculated. (3) Results: most stable parameter weight....

To date, the massive quantity of data generated by high-throughput techniques has not yet met bioinformatics treatment required to make full use it. This is partially due a mismatch in experimental and analytical study design but primarily lack adequate approaches. When integrating multiple types e.g. transcriptomics metabolomics, multidimensional statistical methods are currently choice. Typical approaches, such as canonical correlation analysis (CCA), that applied find associations between...

AbstractThe "fuzzy oil drop" model assumes that the idealized hydrophobic core in a protein body can be described by 3D Gauss function. The structure of 1ICF (cathepsin), which participates proteolysis process and has cysteine-type peptidase activity, been analyzed on basis model. authors have determined contribution individual exon fragments to creation common assessed involvement each chain this process, depending number complexed chains. Quantitative assessment exons, chains, dimers,...