A5028071549- Neuroinflammation and Neurodegeneration Mechanisms
- Geochemistry and Geologic Mapping
- Cell Image Analysis Techniques
- Geological and Geochemical Analysis
- 3D Printing in Biomedical Research
- earthquake and tectonic studies
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neural Engineering
- Industrial Vision Systems and Defect Detection
- Alzheimer's disease research and treatments
- Genomic variations and chromosomal abnormalities
- Chromosomal and Genetic Variations
- Cellular Mechanics and Interactions
- Hydrocarbon exploration and reservoir analysis
- Geological and Geophysical Studies Worldwide
- Image Processing Techniques and Applications
- Immune cells in cancer
- S100 Proteins and Annexins
- Immune Response and Inflammation
- Image and Object Detection Techniques
- Inflammation biomarkers and pathways
- Genome Rearrangement Algorithms
- DNA and Biological Computing
- Neurological Disease Mechanisms and Treatments
- Digital Image Processing Techniques
University of Antwerp
2023-2025
Brooklyn Hospital Center
2025
Icahn School of Medicine at Mount Sinai
2025
KU Leuven
1980-2024
Leuven Institute for Fertility and Embryology
2018-2024
VIB-KU Leuven Center for Brain & Disease Research
2018-2019
Vlaams Instituut voor Biotechnologie
2019
Abstract Microglia are central players in Alzheimer’s disease pathology but analyzing microglial states human brain samples is challenging due to genetic diversity, postmortem delay and admixture of pathologies. To circumvent these issues, here we generated 138,577 single-cell expression profiles stem cell-derived microglia xenotransplanted the App NL-G-F model amyloid wild-type controls. Xenografted adopt a disease-associated profile similar that seen mouse microglia, display more...
Abstract Introduction Murine microglia expressing the Alzheimer's disease–linked TREM2 R47H mutation display variable decrease in phagocytosis, while impaired phagocytosis is reported following loss of TREM2. However, no data exist on +/R47H human microglia. Therefore, we created pluripotent stem cell (hPSC) monocytes and transdifferentiated microglia‐like cells (tMGs) to examine effect phagocytosis. Methods We generated isogenic , +/− −/− hPSCs using CRISPR/Cas9. Following differentiation...
Long considered to fluctuate between pro- and anti-inflammatory states, it has now become evident that microglia occupy a variegated phenotypic landscape with relevance aging neurodegeneration. However, whether specific microglial subsets converge in or contribute both processes eventually affect brain function is less clear. To investigate this, we analyzed heterogeneity tauopathy mouse model (K18-seeded P301L) an accelerated (Senescence-Accelerated Mouse-Prone 8, SAMP8) using cellular...
Abstract Astrocytes, the main supportive cell type of brain, show functional impairments upon ageing and in a broad spectrum neurological disorders. Limited access to human astroglia for pre-clinical studies has been major bottleneck delaying our understanding their role brain health disease. We demonstrate here that functionally mature astrocytes can be generated by SOX9 overexpression 6 days pluripotent stem (PSC)-derived neural progenitor cells. Inducible (i)SOX9-astrocytes display...
Induced pluripotent stem cell (iPSC) technology is revolutionizing biology. However, the variability between individual iPSC lines and lack of efficient to comprehensively characterize iPSC-derived types hinder its adoption in routine preclinical screening settings. To facilitate validation culture composition, we have implemented an imaging assay based on painting convolutional neural networks recognize dense mixed cultures with high fidelity. We benchmarked our approach using pure...
Induction of meiotic competence is a major goal the controlled ovarian stimulation used in ART. Do factors intrinsic to oocyte contribute maturation? Deletions mtDNA accumulate long-lived post mitotic tissues and are found human oocytes. If oogenesis cleanses germline deleterious deletions mtDNA, meiotically competent oocytes should contain lower levels vs. arrested We tested this hypothesis using novel PCR assay for deletion ratio derived from IVF. A real-time was developed measure total...
(Abstracted from J Assist Reprod Genet 2025;42:753–762) Oxidative phosphorylation (OXPHOS) occurs within the mitochondria to produce ATP and is driven by mitochondrial DNA (mtDNA). Mutations in mtDNA have been associated with rare genetic disorders as well cellular dysfunction, these mutations often attributable limited capacity for repair proximity reactive oxygen species generated during OXPHOS.
Microglial activation and neuroinflammation are initial steps in the pathogenesis of Alzheimer’s disease (AD). However, studies mouse models human postmortem samples have yielded divergent results regarding microglia cell states relevant to AD. Here, we investigate 127,000 single expression profiles isolated freshly from a xenotransplantation model for early While adopt disease-associated (DAM) profile, they display much more pronounced HLA-cell state related antigen presentation response...
A system for machine assisted karyotyping and chromosome analysis has been developed. The uses a drum- or TV-scanner as input device, runs provisionally in 32 K memory, also allows human interaction on several stages. accuracy with which banded chromosomes are karyotyped depends strongly the type of classifier varies from 40 up to 80%. (98%) comes close that skilled technician (99.5%) using manual chromosomal analysis. Due technical memory limitations, time necessary one cell is too long...
Induced pluripotent stem cell (iPSC) technology is revolutionizing biology. However, the variability between individual iPSC lines and lack of efficient to comprehensively characterize iPSC-derived types hinder its adoption in routine preclinical screening settings. To facilitate validation culture composition, we have implemented an imaging assay based on painting convolutional neural networks recognize dense mixed cultures with high fidelity. We benchmarked our approach using pure...
Summary paragraph Genetics highlight the central role of microglia in Alzheimer’s disease but at least 36% AD-risk genes lack good mouse orthologues. Here, we show that embryonic stem cell (ESC)-derived human successfully engraft brain and recapitulate transcriptionally primary derived from surgical samples. Upon exposure to oligomeric Aβ a wide range are expressed not readily studied current models for AD. This work provides unique humanized animal model will allow elucidating genetic risk...
In many production chains visual inspection of products is an important manufacturing consideration with respect to quality control. Recent progress in image processing and pattern recognition led the way economically justified applications: modern technology enables construction such automata featuring high reliability constancy. This paper describes automatic machine performing intelligent control tasks very short time. The possible applications include dimension products, objects on...