A5003915697- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Food Allergy and Anaphylaxis Research
- Bacterial Infections and Vaccines
- Allergic Rhinitis and Sensitization
- Toxin Mechanisms and Immunotoxins
- COVID-19 Clinical Research Studies
- Asthma and respiratory diseases
- Long-Term Effects of COVID-19
- Nanoparticle-Based Drug Delivery
- Immunodeficiency and Autoimmune Disorders
- HIV Research and Treatment
- Hepatitis C virus research
- IL-33, ST2, and ILC Pathways
- COVID-19 and Mental Health
- Bacterial Genetics and Biotechnology
- Transgenic Plants and Applications
- Biochemical and Structural Characterization
- Drug Transport and Resistance Mechanisms
- vaccines and immunoinformatics approaches
- Ion Transport and Channel Regulation
Siriraj Hospital
2018-2024
Mahidol University
2018-2024
Health & Life (Taiwan)
2023
National Science and Technology Development Agency
2010-2022
Chiang Mai University
2010-2020
National Center for Genetic Engineering and Biotechnology
2010-2019
Allergen-specific immunotherapy (AIT) facilitates long-term resolution of allergic morbidity resulting in reduced drug use and increased refractoriness to new sensitization. AIT effectiveness has been demonstrated seasonal perennial allergies, insect stings. However, data studies relative cockroach (CR) allergy are relatively scarce. In this study, mice American CR (Periplaneta americana) were treated with a liposome (L)-entrapped vaccine made mouse Tregitope289-Per 9 the CR,...
CD99, a leukocyte surface glycoprotein, is broadly expressed in many cell types. On the surface, CD99 as two distinct isoforms, long form and short form. has been demonstrated to play key role several biological processes, including regulation of T activation. However, molecular mechanisms by which participates such processes are unclear. As contains cytoplasmic tail, it unlikely that itself takes part its multi-functions. Association with other membrane proteins suggested be necessary for...
Abstract Targeting bacterial virulence factors directly provides a new paradigm for the intervention and treatment of diseases. Pseudomonas aeruginosa produces myriad to cause fatal diseases in humans. In this study, human single-chain antibodies (HuscFvs) that bound P. exotoxin A (ETA) were generated by phage display technology using recombinant ETA, ETA-subdomains synthetic peptide ETA-catalytic site as baits selecting ETA-bound-phages from human-scFv library. ETA-bound HuscFvs derived...
Porcine epidemic diarrhea virus (PEDV) is the causative agent of a highly contagious enteric disease pigs characterized by diarrhea, vomiting, and severe dehydration. PEDV infects all ages, but neonatal during first week life are susceptible; mortality rates among newborn piglets may reach 80-100%. Thus, regarded as one most devastating pig viruses that cause huge economic damage to industries worldwide. Vaccination sows gilts at pre-fertilization or pre-farrowing stage good strategy for...
RNA-dependent RNA polymerase (RdRp) is a unique and highly conserved enzyme across all members of the virus superfamilies. Besides, humans do not have homolog this protein. Therefore, RdRp an attractive target for broadly effective therapeutic agent against viruses. In study, formerly generated cell-penetrating human single-chain antibody variable fragment (superantibody) to conformational epitope hepatitis C (HCV) RdRp, which inhibited activity leading HCV replication inhibition host innate...
Autoantibodies against interferon-gamma (IFN-γ) can cause immunodeficiency and are associated with various opportunistic infections. In the present study, we investigated other cellular immune parameters for a better understanding of condition in patients. The numbers WBC, monocytes NK cells were increased patients anti-IFN-γ autoantibodies (AAbs). Upon TCR activation, T cell proliferation IL-2 receptor remained intact. Nonetheless, Th1 cytokine (IFN-γ TNF-α) production was up-regulated. Th2...
Engineered nanobodies (VHs) to the SARS-CoV-2 receptor-binding domain (RBD) were generated using phage display technology. A recombinant Wuhan RBD served as bait in panning fish out nanobody-displaying phages from a VH/VHH library. Sixteen phage-infected E. coli clones produced with 81.79-98.96% framework similarity human antibodies; thus, they may be regarded nanobodies. Nanobodies of 114 and 278 neutralized infectivity dose-dependent manner; 103 105 enhanced virus's by increasing...
Upon generation of monoclonal antibodies to the T cell antigen receptor/CD3 (TCR/CD3) complex, we isolated mAb MT3, whose reactivity correlates inversely with production IFN-γ by human peripheral blood lymphocytes. Using eukaryotic expression cloning, identified MT3 as myelin-and-lymphocyte (MAL) protein. Flow cytometry analysis demonstrates high surface MAL on all naïve CD4+ cells whereas is diminished central memory- and almost lost effector memory cells. MAL- proliferate strongly in...
The quorum sensing (QS) signaling molecule, N-(3-oxododecanoyl)-L-homoserine lactone (3O-C12-HSL), contributes to the pathogenesis of Pseudomonas aeruginosa by regulating expression bacterial virulence factors that cause intense inflammation and toxicity in infecting host. As such, QS molecule is an attractive therapeutic target for direct-acting inhibitors. Several substances, both synthetic naturally-derived products, have shown effectiveness against detrimental 3O-C12-HSL activity....
Broadly effective and safe anti-coronavirus agent is existentially needed. Major protease (3CLpro) a highly conserved enzyme of betacoronaviruses. The plays pivotal role in the virus replication cycle. Thus, it good target broadly anti-Betacoronavirus agent. In this study, human single-chain antibodies (HuscFvs) SARS-CoV-2 3CLpro were generated using phage display technology. 3CLpro-bound phages used to infect Escherichia coli host for production HuscFvs. Computerized simulation was guide...
Allergen-specific-immunotherapy (ASIT) can cause long-term resolution of allergic diseases, reduces drug use and chances new allergen sensitization. Nevertheless, therapeutic vaccine data on ASIT efficacy for cockroach (CR) allergy are relatively scarce. In this study, mechanism a novel intranasal consisting liposome (L)-entrapped mixture American CR (Periplaneta americana) major (Per 9) immunosuppressive protein Brugia malayi nematode named transforming growth factor-beta homologue (TGH) in...
HIV-1 progeny are released from infected cells as immature particles that unable to infect new cells. Gag-Pol polyprotein dimerization via the reverse transcriptase connection domain (RTCDs) is pivotal for proper activation of virus protease (PR protein) in an early event maturation process. Thus, RTCD a potential therapeutic target broadly effective anti-HIV agent through impediment maturation. In this study, human single-chain antibodies (HuscFvs) bound were generated using phage display...
Proviral integration site of Moloney virus-2 (PIM2) is overexpressed in multiple human cancer cells and high level related to poor prognosis; thus, PIM2 kinase a rational target anti-cancer therapeutics. Several chemical inhibitors targeting PIMs/PIM2 or their downstream signaling molecules have been developed for treatment different cancers. However, off-target toxicity common clinical trials, so they could not be advanced official approval application. Here, we produced single-chain...
Many patients develop post-acute COVID syndrome (long (LC)). We compared the immune response of LC and individuals with post-COVID full recovery (HC) during Omicron pandemic. Two hundred ninety-two confirmed infections from January to May 2022 were enrolled. observed anti-SARS-CoV-2 receptor-binding domain immunoglobulin G, surrogate virus neutralization test, T cell subsets, neutralizing antibodies against Wuhan, BA.1, BA.5 viruses (NeuT). NeuT was markedly reduced BA.1 in HC groups, while...
Abstract Natural killer (NK) cells are innate lymphoid having potent cytolytic function that provide host defense against microbial infections and tumors. Using our generated monoclonal antibody (mAb), named FE-1H10, new NK cell sub-populations in peripheral blood were identified. The molecules recognized by mAb FE-1H10 expressed on a sub-population of CD3−CD56dim cells. epitope was demonstrated to be N-glycan proven different from CD57. Upon K562 stimulation, the CD56dimFE-1H10+ exhibited...
Several molecules are known to be involved in T-cell activation via the TCR/CD3 complex and while mechanisms of late T cell signaling have been well characterized, very early events still not fully understood.The aim this study was identify yet unknown associated with complex.To new complex, a monoclonal antibody termed MT3 produced by immunoprecipitated beads immunization. Colocalization mAb recognizing complexes verified confocal microscopic analysis. The surface antigen recognized...
RNA-dependent RNA polymerase (RdRp) is a good target of anti-RNA virus agents; not only it pivotal for the replication cycle and highly conserved among viruses across different families, but also lacks human homolog. Recently, single-chain antibody (HuscFv) that bound to thumb domain hepatitis C (HCV) (functionalized NS5B protein) was produced engineered into cell-penetrating (super antibody) in form peptide (penetratin, PEN)-linked HuscFv (PEN-HuscFv34). The super purified from inclusion...
<title>Abstract</title> Humans get SARS-CoV-2 infection through inhalation; thus, vaccine that induces protective immunity at the virus entry site is appropriate for early control of infection. In this study, two anionic liposome-adjuvanted VLPs vaccines made full-length S, M and E proteins were formulated. S1-S2 junction S protein displayed on one (L-SME-VLPs) contained furin cleavage site, while another (L-S¢ME-VLPs) did not. Both similarly/equally immunogenic in mice. Mice immunized...
Humans get SARS-CoV-2 infection mainly through inhalation; thus, vaccine that induces protective immunity at the virus entry site is important for early control of infection. In this study, two anionic liposome (L)-adjuvanted VLP vaccines against were formulated. Baculovirus-Sf21 insect cell system was used production VLPs made full-length S, M and E proteins. S protein one (L-SME-VLPs) contained furin cleavage S1/S2 junction, while another (L-S'ME-VLPs) did not. Both innocuous immunogenic...
ABSTRACT BACKGROUND In a number of patients, post-acute COVID syndrome develops after acute infection with severe respiratory coronavirus 2 (SARS-CoV-2) (Long [LC]). Here, we examined the immune responses and clinical characteristics individuals LC compared to age- gender-matched healthy recovered (HC) during Omicron pandemic. Immune following BNT162b2 (Pfizer) booster are also determined. METHODS This retrospective cohort study included 292 patients (LC, 158; HC, 134) confirmed have...
Event Abstract Back to Suppression of T Cells Proliferation by A Novel Generated Monoclonal Antibody, COS3A Siriwan Wansook1, Supansa Pata2, Kodchakorn Mahasongkram2, Watchara Kasinrerk2 and Panida Khunkaewla1* 1 Suranaree university Technology, Biochemistry-Electrochemistry Research Unit, School Biochemistry, Institute Science, Thailand 2 Chiang Mai University, Division Clinical Immunology, Department Medical Faculty Associated Sciences, Leukocytes surface molecules play crucial role in...