A5082342467- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Cancer, Stress, Anesthesia, and Immune Response
- Gut microbiota and health
- Toxin Mechanisms and Immunotoxins
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- HER2/EGFR in Cancer Research
- CAR-T cell therapy research
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
- Histone Deacetylase Inhibitors Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related cognitive impairment studies
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Fibroblast Growth Factor Research
- Galectins and Cancer Biology
- Colorectal Cancer Treatments and Studies
- IL-33, ST2, and ILC Pathways
- Microscopic Colitis
The University of Texas MD Anderson Cancer Center
2018-2022
The University of Texas Health Science Center at Houston
2020-2022
Robin Medical (United States)
2016-2017
For Robin (United States)
2016-2017
University at Buffalo, State University of New York
2017
Type 1 conventional dendritic cells (cDC1s) possess efficient antigen presentation and cross-presentation activity, as well potent T cell priming ability. Tissue-resident cDC1s (CD103+ in mice, CD141+ humans) are linked with improved tumor control, yet the efficacy of immunotherapy using this population is understudied.We generated murine CD103+ vitro examined their expression cDC1-related factors, accumulation tumor-draining lymph nodes (TdLNs). The antitumor vitro-generated was studied...
Immune checkpoint blockade (ICB) has revolutionized cancer treatment, yet quality of life and continuation therapy can be constrained by immune-related adverse events (irAEs). Limited understanding irAE mechanisms hampers development approaches to mitigate their damage. To address this, we examined whether mice gained sensitivity anti-CTLA-4 (αCTLA-4)–mediated toxicity upon disruption gut homeostatic immunity. We found αCTLA-4 drove increased inflammation colonic tissue damage in with...
Breast cancer is the most common female malignancy in both developed and developing world.Doxorubicin one of commonly used chemotherapies for breast cancer.Unfortunately, up to 60% survivors report long-term chemotherapy-induced cognitive dysfunction (CICD) characterized by deficits working memory, processing speed executive function.Currently, no therapeutic standard treating CICD exists.Here, we hypothesized that treatment with a blood-brain barrier permeable histone deacetylase 6 (HDAC6)...
Conventional dendritic cells (cDCs) are a critical immune population, composed of multiple subsets, and responsible for controlling adaptive immunity tolerance. Although migratory type 1 cDCs (CD103+ cDC1s in mice) necessary to mount CD8+ T cell-mediated anti-tumor immunity, whether how tumors modulate CD103+ cDC1 function remain understudied. Signal Transducer Activator Transcription 3 (STAT3) mediates the intracellular signaling tumor-associated immunosuppressive cytokines, such as...
Blood cell formation must be appropriately maintained throughout life to provide robust immune function, hemostasis, and oxygen delivery tissues, prevent disorders that result from over- or underproduction of critical lineages. Persistent inflammation deregulates hematopoiesis by damaging hematopoietic stem progenitor cells (HSPCs), leading elevated myeloid output eventual bone marrow failure. Nonetheless, antiinflammatory mechanisms protect the system are understudied. The transcriptional...
JAA-F11 is a highly specific mouse monoclonal to the Thomsen-Friedenreich Antigen (TF-Ag) which an alpha-O-linked disaccharide antigen on surface of ~80% human carcinomas, including breast, lung, colon, bladder, ovarian, and prostate cancers, cryptic normal cells. has potential, when humanized, for cancer immunotherapy multiple types. Humanization JAA-F11, was performed utilizing complementarity determining regions grafting homology framework. The objective herein test specificity, affinity...
Abstract Type I conventional dendritic cells (cDC1s) are an essential Ag-presenting population required for generating adaptive immunity against intracellular pathogens and tumors. While the transcriptional control of cDC1 development is well understood, mechanisms by which extracellular stimuli regulate function remain unclear. We previously demonstrated that cytokine-responsive regulator STAT3 inhibits polyinosinic:polycytidylic acid [poly(I:C)]-induced maturation cDC1-mediated antitumor...
Abstract Type 1 conventional dendritic cells (cDC1s) possess efficient antigen presentation and cross-presentation activity, yet the efficacy of immunotherapy utilizingthis population is understudied. We used in vitro-generated CD103+cDC1s vaccination strategies to test their ability control melanoma osteosarcoma tumors. In produced cDC1-associated factors such as IL-12p70 CXCL10, demonstrated activity upon stimulation with Toll-like receptor 3 (TLR3) agonist polyinosinic:polycytidylic acid...
Immunotherapies such as anti-CTLA-4 (aCTLA-4) immune checkpoint blockade (ICB) have revolutionized cancer treatment, yet quality of life and continuation therapy can be constrained by off-target tissue damage or immune-related adverse events (irAEs).At present, there is limited understanding irAE mechanisms, hampering development approaches to mitigate their damage.We addressed this problem generating animal models intestinal irAE.Our results show that disruption homeostatic immunity genetic...
e14566 Background: Immune checkpoint blockade (ICB) therapy transformed clinical oncology by inducing durable responses and increasing survival rates in many types of cancers. However, ICB is effective only a subset patients. Recent studies delineated the role gut microbiome as both biomarker therapeutic responsiveness. We aimed to increase understanding microbiome-immune system axis using antibiotics knock out certain components microbiome. Methods: treated MC38 colon adenocarcinoma-bearing...
Abstract Conventional dendritic cells (cDCs) are a crucial immune population, which includes multiple subtypes that coordinate adaptive immunity and tolerance. Migratory type 1 cDC1s (CD103+ in mice) required to induce CD8+ T cell-mediated anti-tumor immunity, yet whether how tumors regulate CD103+ cDC1 function is largely unknown. Many tumor-associated immunosuppressive cytokines, such as interleukin (IL)-10, require Signal Transducer Activator of Transcription 3 (STAT3) for intracellular...
Abstract Theranostics through the utilization of immunohistochemistry followed by radioimaging to determine if metastatic foci will react with a therapeutic antibody allow for selection patient population that most benefit from this immunotherapy. The Thomsen-Friedenreich antigen (TF-Ag) has been shown be involved in ∼90% carcinomas, specifically breast making it suitable target and therapy. anti-TF-Ag antibody, JAA-F11, mouse monoclonal (mAb), had success localization, blocking metastasis,...
Abstract Background: Chronic inflammation, a hallmark of cancer, is associated with poor prognosis in human various malignancies. Predominantly, tumor neutrophils (TANs) and myeloid-derived suppressor cells (MDSCs) are immune well characterized to promote support growth metastasis, by secreting chemokines, serine proteases reactive oxygen species. Increased TANs MDSCs predictive markers both progression suggesting that their inhibition may provide viable anti-tumor strategies. Neutrophil...
Abstract Intestinal infections trigger immune responses that in some cases become dysregulated and lead to acute or chronic disease; understanding regulatory mechanisms infection will help advance new therapies for intestinal disorders. Citrobacter rodentium mice mimics enteropathogenic Escherichia coli humans. We found Leukemia inhibitory factor (LIF), a member of the interleukin-6 (IL-6) cytokine family, is upregulated colon serum following C. infection; however, source, targets effects...
Abstract Plasmacytoid dendritic cells (pDCs) are specialized type I interferon (IFN-I) producing that mediate anti-viral responses, anti-tumor immunity, and autoimmunity. When exposed to Toll-like receptor 7 (TLR7)- TLR9-ligands, pDCs mature produce IFN-Is; also acquire conventional DC (cDC)-like morphology features, including the cell surface expression of antigen presentation co-stimulatory molecules, secretion additional cytokines, ability activate adaptive T responses. Yet,...
Abstract Immunotherapies such as anti-CTLA-4 immune checkpoint blockade (ICB) have revolutionized cancer treatment, yet quality of life and continuation therapy can be constrained by off-target tissue damage or immune-related adverse events (irAEs). At present, there is limited understanding irAE mechanisms, hampering development approaches to mitigate their damage. We addressed this problem generating animal models intestinal irAE. Our results show that disruption homeostatic immunity...
Abstract Conventional dendritic cells (cDCs) are the primary antigen presenting of immune system, and thus control adaptive immunity. Type 1 cDCs (cDC1s) crucial for mounting protective T cell responses against tumors viruses. Recently, we reported that interleukin (IL)-10 inhibits polyinosinic-polycytidylic acid (poly I:C; a toll-like receptor 3 agonist) induced maturation cDC1s in signal transducer activator transcription (STAT) 3-dependent manner. In addition, using tumor vaccine...
Abstract The inhibitor of DNA binding (Id) proteins and E are basic helix-loop-helix that act as mutual antagonists to direct immune cell development function. In developing dendritic cells (DCs), Id2 specifies type 1 conventional DCs (cDC1s) represses E2-2, an protein master regulator plasmacytoid (pDCs). detect viruses, bacteria, tumor via pattern recognition receptors, such Toll-like receptors (TLRs), elicit responses. Previously, we found mRNA was induced in TLR agonist-stimulated murine...
Abstract Type 1 conventional dendritic cells (cDC1s) have critical roles in inducing adaptive immune responses and mediating tolerance. Signal Transducer Activator of Transcription 5 (STAT5) was found to be crucial for monocyte-derived DC development; however, its role non-lymphoid tissue CD103+cDC1s remained largely unknown. We evaluated the STAT5 by employing myeloid-specific Stat5-deficient mice,LysM-Cre +Stat5f/f CD11c-Cre+Stat5f/f mice. Both strains show deficiencies numbers alveolar...