A5043853633- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- Advanced Biosensing Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Salivary Gland Disorders and Functions
- Advanced Proteomics Techniques and Applications
- Immunotherapy and Immune Responses
- Rheumatoid Arthritis Research and Therapies
- SARS-CoV-2 and COVID-19 Research
- Systemic Sclerosis and Related Diseases
- Lymphoma Diagnosis and Treatment
- Inflammatory Myopathies and Dermatomyositis
- Immunodeficiency and Autoimmune Disorders
- vaccines and immunoinformatics approaches
- Glycosylation and Glycoproteins Research
- Diabetes and associated disorders
- Cell Adhesion Molecules Research
- Long-Term Effects of COVID-19
- Peptidase Inhibition and Analysis
- Hepatitis C virus research
- Medical Imaging and Pathology Studies
- Bladder and Urothelial Cancer Treatments
- COVID-19 Clinical Research Studies
- Advanced biosensing and bioanalysis techniques
OncoImmune (United States)
2024
Protagen (Germany)
2013-2022
Proteome Sciences (United Kingdom)
2017
Photonics Electronics Technology Research Association
2015
PXBioVisioN (Germany)
2005-2008
Bielefeld University
1994-2006
Pronounced sex differences in the susceptibility and response to SARS-CoV-2 infection remain poorly understood. Emerging evidence has highlighted potential importance of autoimmune activation modulating acute recovery trajectories following exposure. Given that immune-inflammatory activity can be sex-biased setting severe COVID-19 illness, aim study was examine sex-specific reactivity absence extreme clinical disease.In this study, we assessed autoantibody (AAB) 91 autoantigens previously...
Abstract To evaluate the potential role of human placental endothelial cells in transport IgG from maternal to fetal circulation, we studied Fcγ receptor (FcγR) expression by immunohistology and immunoblotting. Several pan-FcγRII Abs that label endothelium displayed a distribution pattern correlated well with functions, being intense terminal villus nil cord. In contrast, MHC class 1-like transporter, FcRn, classical FcγRIIa were not expressed transport-related placenta. Our inference,...
Systemic lupus erythematosus (SLE) is a heterogeneous disease with respect to manifestations, progression and treatment response. Therefore, strategies identify biomarkers that help distinguishing SLE subgroups are major focus of biomarker research. We reasoned multiparametric autoantibody profiling approach combined data mining tools could be applied patient clusters. used bead-based array containing 86 antigens including diverse nuclear immune defense pathway proteins. Sixty-four...
Human B cells express four immunoglobulin G receptors, FcgammaRIIa, FcgammaRIIb1, FcgammaRIIb2, and FcgammaRIIc. Coligation of either FcgammaRII isoform with the B-cell antigen receptor (BCR) results in abrogation activation, but only FcgammaRIIa/c FcgammaIIb1 isoforms become phosphorylated. To identify FcgammaRII-phosphorylating protein tyrosine kinase (PTK), we used combination an vitro vivo approach. In assay using recombinant cytoplasmic tails different as well exchange mutants, show...
Introduction The diagnosis of Sjögren’s disease (SjD) in patients without autoantibodies against Ro/SSA is a major challenge. We aimed to identify novel SjD that may facilitate the diagnostic procedure for negative SjD. Methods IgG and IgA autoantibody reactivity 94 potential candidate autoantigens SjD, selected from discovery screen 1,629 human antigens coupled Luminex beads prior knowledge about biological relevance, were examined serum (n=347) using ELISA technology. Healthy (HC, n=118)...
Human B cells express two closely related immunoglobulin G receptors, FcRIIb1 and FcRIIb2, which differ by a 19 amino acid insertion in the cytoplasmic tail of FcRIIb1. The tails both isoforms contain conserved sequence motif (AENTITYSLL) essential for mediating endocytosis via FcRIIb2. Truncation this abolished endocytosis, while replacement tyrosine (Tyr273) FcRIIb2 phenylalanine had no effect on amount kinetics ligand uptake. Co-cross-linking or with antigen receptor led to an abortive...
Transient receptor potential proteins (TRP) are supposed to participate in the formation of store‐operated Ca 2+ influx channels by co‐assembly. However, little is known which domains facilitate interaction subunits. Contribution N‐terminal coiled‐coil domain and ankyrin‐like repeats putative pore region mouse TRP1β (mTRP1β) variant functional cation were analyzed following overexpression HEK293 (human embryonic kidney) cells. MTRP1β expressing cells exhibited enhanced whole‐cell membrane...
Objective Activation of endosomal toll‐like receptors (TLRs) is one possible driver inflammation in idiopathic inflammatory myopathies (IIM). We investigated the potential contribution TLR7 and TLR8 to IIM pathogenesis. Methods TLR7/8 healthy donor peripheral blood mononuclear cells (PBMCs) by immune complexes from patients with lupus was tested. Autoantibody profiling patient IgG samples performed using a 1581‐antigen array. and/or activation RNA molecules associated autoantibodies...
Reactive astrocytes occurring in response to neurodegeneration are thought play an important role neuronal regeneration by upregulating the expression of extracellular matrix (ECM) components as well ECM degrading metalloproteinases (MMPs). We examined mRNA levels and cellular distribution membrane type metalloproteinase 1 (MT1-MMP) tissue inhibitors 1-4 MMPs (TIMPs) brain stem spinal cord wobbler (WR) mutant mice affected progressive astrogliosis. MT1-MMP, TIMP-1 TIMP-3 were elevated,...
The aim was to identify novel diagnostic autoantibody candidates for rheumatoid arthritis (RA) by comprehensive screening autoreactivity. We incubated 5892 recombinant proteins coupled fluorescent beads, with patients' sera the detection of IgG-autoantibodies in three independent patient cohorts: A (n = 72 patients established RA); B/B- 116 early RA (B) and n 51 CCP-negative from B (B-)); C 184 seronegative RA), comparison matched healthy controls. Intersects significantly increased...
Type 2 diabetes mellitus (T2DM) is caused by the failure of pancreatic beta-cell to secrete sufficient insulin compensate a decreased response peripheral tissues action. The pathological events causing dysfunctions are only poorly understood and early markers that would predict islet function missing. In contrast immunoassays, unbiased proteomic technologies provide opportunity screen for novel marker protein peptides T2DM. An important subset proteome, peptide hormones secreted pancreas...
Diagnosis of SLE relies on the detection autoantibodies. We aimed to assess diagnostic potential histone H4 and H2A variant antibodies in SLE. IgG-autoantibodies histones (HIST1H4A), type 2-A (HIST2H2AA3) 2-C (HIST2H2AC) were measured along with a standard antibody (SA) set including SSA, SSB, Sm, U1-RNP RPLP2 multiplex magnetic microsphere-based assay 153 patients [85% female, 41 (13.5) years] 81 healthy controls [77% 43.3 (12.4) years]. Receiver operating characteristic analysis was...
Mass spectrometric plasma analysis for biomarker discovery has become an exploratory focus in proteomic research: the challenges of analyzing samples by mass spectrometry have apparent not only since human proteome organization (HUPO) put much emphasis on proteome. This work demonstrates fundamental research to reveal sensitivity and quantification capabilities our Peptidomics technologies detecting distinct changes peptide composition after challenging healthy volunteers with orally...
The 7th Ammendment to the Cosmetics Directive of European Commission (Directive 76/768/EEC2) bans marketing cosmetics containing animal-tested ingredients since March 2009. Excepted are only tests for repeated dose toxicity, which animal ban will come into effect by 2013. One major concern cosmetics, i.e. risk skin (contact) sensitizers, has in past been addressed almost exclusively testing. It is this problem attracting central interest integrated research project Sens-it-iv (Novel Testing...
Cutaneous lupus erythematosus (CLE) is an inflammatory autoimmune skin disease in which abnormal photosensitivity important pathogenetic factor but difficult to predict, creating a challenge determining treatment efficacy. Although CLE patients may change over time, photoprovocation testing with ultraviolet (UV) A and UVB irradiation can be helpful tool explore differences between responders nonresponders during photoprovocation. To identify biomarkers that could substitute for the clinical...
Abstract Seropositivity for anti-citrullinated peptide antibodies (ACPA) in patients with rheumatoid arthritis (RA), a chronic autoimmune arthritis, is associated worse long-term disease outcomes. ACPA ubiquitously tested RA patients, but other autoantibodies exist (in both citrullinated and non-citrullinated form) which may provide additional information on subtypes and/or treatment response. We used multiplex bead-based assay of 376 to test associations between these response patients....
10011 Background: Increasing evidence suggests that the B cell response in cancer patients is an important component of anti-tumour immunity. Autoantibodies targeting tumour and self-antigens may serve as biomarkers auto-immunity. As they can be measured patient' sera, have great potential clinical routine biomarkers. The objective this study was to explore if autoantibodies are associated with survival immune related adverse events (irAE) metastatic melanoma under checkpoint inhitibor (CPI)...
Comprehensive profiling of autoantibodies (AAbs) in metastatic urothelial cancer (mUC) has not been performed to date. This may aid diagnosis UC, uncover novel therapeutic targets this disease as well identify associations between AAbs and response toxicity systemic therapies. We used serum from patients with mUC collected prior after therapy (immune checkpoint inhibitor (ICI) or platinum-based chemotherapy (PBC)) at Dana-Farber Cancer Institute. 38 age-matched sex-matched healthy controls...
Objective To investigate the role of epitope spreading in established systemic lupus erythematosus ( SLE ). Methods IgG autoantibody reactivity with 398 distinct recombinant proteins was measured over a period 6 years 69 patients and compared to that 45 controls. Changes mean fluorescence intensity MFI ), number autoantibodies antigens, clones antigenic targets (e.g., U1 RNP , Sm, ribosomal P) representing fine mapping were assessed. Linear mixed modeling, adjusted Bonferroni correction for...
Detection and purification of novel bioactive peptides from biological sources is a scientific task that led to substantial number important discoveries. One major laborious approach used the repetitive stepwise separation test sample into several fractions followed by determination their bioactivity, until purity allows for sequence identification. We tested whether functional peptidomics, combination read-outs with differential peptide display (DPD) suitable strategy isolate at lower...