A5056813121- Effects of Radiation Exposure
- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Radiation Therapy and Dosimetry
- Radiation Dose and Imaging
- Glutathione Transferases and Polymorphisms
- Genomics, phytochemicals, and oxidative stress
- Adipose Tissue and Metabolism
- Head and Neck Cancer Studies
- Radiation Effects and Dosimetry
- Polyomavirus and related diseases
- Nuclear Receptors and Signaling
- RNA Research and Splicing
- NF-κB Signaling Pathways
- Radioactive contamination and transfer
- Nuclear Physics and Applications
- Genomics and Chromatin Dynamics
- Biotin and Related Studies
- Cervical Cancer and HPV Research
- Immune Cell Function and Interaction
- Oral health in cancer treatment
- Health Systems, Economic Evaluations, Quality of Life
- Viral-associated cancers and disorders
- Advanced Radiotherapy Techniques
- Nonmelanoma Skin Cancer Studies
Federal Office for Radiation Protection
2011-2024
Klinik und Poliklinik für Psychosomatik und Psychotherapie
2008
University of Cologne
2008
Centrum für Integrierte Onkologie
2006
Ruhr University Bochum
1992-1994
Radiotherapy is a powerful cure for several types of solid tumours, but its application often limited because severe side effects in individual patients. With the aim to find biomarkers capable predicting normal tissue reactions we analysed radiation responses cells from head and neck tumour breast cancer patients different clinical radiosensitivity multicentric study. Multiple parameters cellular were coded samples peripheral blood lymphocytes (PBLs) derived lymphoblastoid cell lines (LCLs)...
Abstract Normal tissue toxicity after radiotherapy shows variability between patients, indicating inter-individual differences in radiosensitivity. Genetic variation probably contributes to these differences. The aim of the present study was determine if two cell lines, one radiosensitive (RS) and another radioresistant (RR), showed DNA repair capacity, viability, cycle progression and, turn, this response could be characterised by a differential gene expression profile at different...
Journal Article The WST survival assay: an easy and reliable method to screen radiation-sensitive individuals Get access A. Guertler, Guertler 1Department SG Radiation Protection Health, Federal Office for Protection, Oberschleissheim, Germany Search other works by this author on: Oxford Academic PubMed Google Scholar Kraemer, Kraemer 2Institute of Biology, Helmholtz Zentrum Munich—German Research Center Environmental Neuherberg, U. Roessler, Roessler S. Hornhardt, Hornhardt Kulka, Kulka...
Radiation-induced alterations in posttranslational histone modifications (PTMs) may affect the cellular response to radiation damage DNA. If not reverted appropriately, altered PTM patterns cause long-term gene expression regulation and thus lead cancer. It is therefore important characterize radiation-induced factors affecting them. A lymphoblastoid cell line established from a normal donor was used screen for methylation levels at H3K4, H3K9, H3K27, H4K20, as well acetylation H3K56, H4K5,...
Purpose: Automated detection of dicentric chromosomes from a large number cells was applied to study age-dependent radiosensitivity after in vitro CT exposure blood healthy donors.Materials and methods: Blood samples newborns, children (2–5 years) adults (20–50 were exposed 0 mGy, 41 mGy 978 using equipment. In this study, automated scoring based on 13,000–31,000 cells/dose point/age group performed. Results for control low dose points validated by manually counting about 26,000...
To establish stable in vitro growth of keratinocytes from very small biopsy specimens and successfully apply new test systems to determine their radiosensitivity.Oral mucosa biopsies (diameter: 1.7 mm) 15 subjects were immobilized with custom-made cups onto culture plates. Outgrowing cells tested for cytokeratin 5/14 Ki67, expanded, radiated at different doses, seeded circumscribed areas before being allowed spread centrifugally. In this newly developed spreading assay, cell-covered measured...
Experiments were designed and performed in order to investigate whether or not the different cellular energy deposition patterns of photon radiation with energies (29 kV, 220 kV X rays; Co-60, Cs-137-gamma-rays) alpha-radiation from an Am-241 source differ DNA damage induction capacity human cells. For this purpose, alkaline comet assay (single cell gel electrophoresis) was applied measure amount relation dose received. The data for parameters '% tail' 'tail moment' peripheral lymphocytes...
Ataxia-telangiectasia (AT) is a rare inherited recessive disorder which caused by mutated (ATM) gene. Hallmarks include chromosomal instability, cancer predisposition and increased sensitivity to ionizing radiation. The ATM protein plays an important role in signaling of DNA double-strand breaks (DSB), thereby phosphorylating the histone H2A.X. Non-functional leads defects damage response, unresolved DSBs genomic instability. aim this study was evaluate aberrations γH2A.X foci as potential...
Plant genomes contain polymorphic repetitive sequences that can be used as DNA markers. Minisatellites (16 to 64 bp per repeat) and simple-sequence repeats (2 6 are the most markers found in plant animal genomes. In this study, hybridizations between genomic variable number of tandem repeat probes were examined Arabidopsis thaliana L. (Heynn) , onion ( Allium cepa L.), tomato Lycopersicon esculentum wheat Triticum aestivum avocado Persea americana Mill.), litchi Chinensis Sonn.), mango...
The Y-Specific Simple Sequence Length Polymorphism (SSLP) of the locus DYS19 was investigated in two Slovakian samples--Bratislava and Medzev--and German samples--Bremen Hannover. allele frequencies show a significant difference between samples.
Head and neck cancer (HNC) accounts for almost 890,000 new cases per year. Radiotherapy (RT) is used to treat the majority of these patients. A common side-effect RT onset oral mucositis, which decreases quality life represents major dose-limiting factor in RT. To understand origin biological mechanisms post-ionizing radiation (IR) need be clarified. Such knowledge valuable develop treatment targets mucositis markers early identification "at-risk" patients.Primary keratinocytes from healthy...
In case of population exposure by ionizing radiation, a fast and reliable dose assessment exposed non-exposed individuals is crucial important. initial triage, physicians have to take decisions whom treat with adequate medical care. addition, worries about significant can be taken away from hundreds thousands non- or low individuals. Studies shown that the γH2A.X radiation-induced foci assay promising test for triage decisions. However, in large-scale scenario most biodosimetry laboratories...
X-ray cabinets are replacing 137 Cs/60 Co sources in radiation biology research due to advantages size, handling, and protection. However, because of their different physical properties, more susceptible experimental influences than conventional sources. The aim this study was examine the variations related setups typically used investigate biological effects with cabinets.A combined approach dose measurements by thermoluminescence dosimetry detection quantification γH2AX 53BP1 foci analyze...
Sensitivity to ionizing radiation differs between individuals, but there is a limited understanding of the biological mechanisms that account for these variations. One example such are mutations in ATM (mutated ataxia telangiectasia) gene, cause rare recessively inherited disease Ataxia telangiectasia (AT). Hallmark features include chromosomal instability and increased sensitivity (IR).
Systematic bio- and databanks are key prerequisites for modern radiation research to investigate response mechanisms in the context of genetic, environmental lifestyle-associated factors. This report presents current status German Uranium Miners’ Biobank. In 2008, databank was established at Federal Office Radiation Protection, sampling biological materials from former uranium miners with without lung cancer initiated. For this purpose, various specimens, such as DNA RNA, were isolated blood...
Abstract The principle of a novel typing techniques is described for screening with high efficiency the “immunological genome” by using various intronic and exonic oligonucleotide hybridization probes simultaneously or subsequently.
Aims: Generation of new adipocytes plays a major role in the development obesity. We previously have shown that transcriptional repressor FBI-1 exerts dual effect process adipogenesis by inhibiting proliferation and promoting differentiation preadipocytes. The aim present study was to identify regulated molecular effectors which could account for these effects.
Fragestellung: Transkriptionsfaktoren spielen eine wichtige Rolle in der humanen und 3T3-L1-Adipogenese. Dabei wurden Vergangenheit vor allem transkriptionelle Aktivatoren (z.B. PPAR-γ) charakterisiert. Neuere Untersuchungen weisen jedoch darauf hin, dass insbesondere beim Übergang von Proliferation zu Differenzierung auch Repressoren Funktion einnehmen. Deshalb sollten im Rahmen des aktuellen Projektes Expressionsprofile für HDAC-1, SMRT, NCoR Sin3A 3T3-L1 Adipogenese erstellt werden.