A5013510344- Advanced Radiotherapy Techniques
- Medical Imaging Techniques and Applications
- DNA Repair Mechanisms
- Effects of Radiation Exposure
- Lung Cancer Treatments and Mutations
- Lung Cancer Diagnosis and Treatment
- Sarcoma Diagnosis and Treatment
- Gastric Cancer Management and Outcomes
- Cancer Diagnosis and Treatment
- Radiation Therapy and Dosimetry
- Medical Imaging and Pathology Studies
- Cancer Treatment and Pharmacology
- Glioma Diagnosis and Treatment
- Management of metastatic bone disease
- Brain Metastases and Treatment
- Radiopharmaceutical Chemistry and Applications
- Hepatocellular Carcinoma Treatment and Prognosis
- Carcinogens and Genotoxicity Assessment
- Radiomics and Machine Learning in Medical Imaging
- Cancer and Skin Lesions
- Breast Cancer Treatment Studies
- Head and Neck Surgical Oncology
- Advances in Oncology and Radiotherapy
- Nonmelanoma Skin Cancer Studies
- Neuroendocrine Tumor Research Advances
Saarland University
2015-2024
Universitätsklinikum des Saarlandes
2005-2011
Universität Hamburg
1999
University West
1999
Ludwig-Maximilians-Universität München
1989
Microglia activated by extracellularly deposited amyloid β peptide (Aβ) act as a two-edged sword in Alzheimer's disease pathogenesis: on the one hand, they damage neurons releasing neurotoxic proinflammatory mediators (M1 activation); other protect triggering anti-inflammatory/neurotrophic M2 activation and clearing Aβ via phagocytosis. TLRs are associated with Aβ-induced microglial inflammatory internalization, but mechanisms remain unclear. In this study, we used real-time surface plasmon...
Accumulation of DNA damage leading to adult stem cell exhaustion has been proposed be a principal mechanism aging. Here we tested this hypothesis in healthy individuals different ages by examining unrepaired double-strand breaks (DSBs) hematopoietic stem/progenitor cells matured their physiological microenvironment.To asses accumulation and repair capacities, γH2AX-foci were examined before after exposure ionizing irradiation. Analyzing CD34+ CD34- observed an increase endogenous levels with...
Das Lungenkarzinom ist mit jährlich über 40 000 Sterbefällen in der Bundesrepublik Deutschland die vierthäufigste Todesursache und häufigste Krebstodesursache [1]. Trotz Fortschritte Diagnostik Therapie liegt 5-Jahres-Überlebensrate Patienten europäischen nordamerikanischen Ländern nur einem Bereich von 5,5 – 15,7 % [2] [3]. Ziel vorliegenden Leitlinie Verbesserung Prognose Lebensqualität Lungenkarzinomen durch Optimierung des Einsatzes derzeitigen diagnostischen therapeutischen...
Abstract The senescence of mammalian cells is characterized by a proliferative arrest in response to stress and the expression an inflammatory phenotype. Here we show that histone H2A.J, poorly studied H2A variant found only mammals, accumulates human fibroblasts with persistent DNA damage. H2A.J also mice aging tissue-specific manner skin. Knock-down inhibits genes contribute senescent-associated secretory phenotype (SASP), over increases some these proliferating cells. accumulation may...
Radiotherapy is an effective cancer treatment, but a few patients suffer severe radiation toxicities in neighboring normal tissues. There increasing evidence that the variable susceptibility to caused by individual genetic predisposition, subtle mutations, or polymorphisms genes involved cellular responses ionizing radiation. Double-strand breaks (DSB) are most deleterious form of radiation-induced DNA damage, and DSB repair deficiencies lead pronounced radiosensitivity. Using preclinical...
Ionizing radiation (IR) induces a variety of DNA lesions among which double-strand breaks (DSBs) are the biologically most significant. It is currently unclear if DSB repair equally efficient after low and high doses. Here, we use γ-H2AX, phospho-ATM (pATM), 53BP1 foci analysis to monitor repair. We show, consistent with previous study, that kinetics γ-H2AX pATM loss in confluent primary human fibroblasts substantially compromised doses 10 mGy lower. Following 2.5 mGy, cells fail show any...
This abridged version is based on an interdisciplinary guideline which corresponds to development stage 3 (S3) of guidelines according the classification Association Scientific Medical Societies in Germany (AWMF). The process characterized by combination formal evidence-search, consensus, logic (algorithms), decision and outcome analysis, with cooperation 15 German Austrian medical societies. [Table 1] shows relationship between levels evidence, resulting recommendation grades...
An important hallmark of radiation dermatitis is the impairment mitotic ability stem/progenitor cells in basal cell layers due to radiation-induced DNA damage, leading suppressed renewal epidermis. However, this mechanism alone does not adequately explain complex pathogenesis skin injury. In review, we summarize latest findings on and correlate these with clinical features reactions. The current studies show that exposure ionizing induces cellular senescence epidermal keratinocytes. As part...
Abstract Cellular senescence is an irreversible growth arrest that occurs as a result of damaging stimuli, including DNA damage and/or telomere shortening. Here, we investigate histone variant H2A.J new biomarker to detect senescent cells during human skin aging. Skin biopsies from healthy volunteers different ages (18–90 years) were analyzed for expression and other parameters involved in triggering maintaining cellular senescence. In the epidermis, proportions H2A.J-expressing...
Radiotherapy (RT) is the primary treatment modality for inoperable, locally advanced non-small-cell lung cancer (NSCLC), but even with highly conformal planning, radiation pneumonitis (RP) remains most serious, dose-limiting complication. Previous clinical reports proposed that cytokine plasma levels measured during RT allow to estimate individual risk of patients develop RP. The identification such profiles would facilitate tailoring radiotherapy maximize efficacy and minimize toxicity....
Die vorliegende Kurzfassung basiert auf einer interdisziplinären Leitlinie, die der Entwicklungsstufe 3 (S3) Leitlinien nach Klassifikation Arbeitsgemeinschaft Wissenschaftlichen Medizinischen Fachgesellschaften (AWMF) entspricht und durch Kombination von formaler Evidenz-Recherche, Konsensfindung, Logik (Algorithmen), Entscheidungs- Outcome-Analyse sowie interdisziplinärem Abgleich unter Beteiligung 15 deutschen österreichischen medizinischen gekennzeichnet ist. [Tab. 1] zeigt Beziehung...
Purpose DNA double-strand breaks (DSBs) generated by ionizing radiation pose a serious threat to the preservation of genetic and epigenetic information. The known importance local chromatin configuration in DSB repair raises question whether different environments are recognized repaired same machinery with similar efficiency. An essential step processing non-homologous end joining is high-affinity binding Ku70-Ku80 DNA-PKcs double-stranded ends that holds physical proximity for subsequent...
Remodeling of extracellular matrix (ECM) after lung damage depends on collagen degrading Matrix-Metallo-Proteinases (MMP) and their endogenous inhibitors (Tissue-Inhibitors Metallo-Proteinases, TIMP). Transforming growth factor (TGF)-beta1 has been implicated in the pathogenesis radiation-induced fibrosis upon its effects fibroblast proliferation synthesis. Lung cancer patients have often elevated TGF-beta1 plasma levels as a result increased expression tumours. On this background, we...
Irreparable double-strand breaks (DSBs) in response to ionizing radiation (IR) trigger prolonged DNA damage (DDR) and induce premature senescence. Profound chromatin reorganization with formation of senescence-associated heterochromatin foci (SAHF) is an essential epigenetic mechanism for controlling the secretory phenotype (SASP). To decipher molecular mechanisms provoking continuous DDR leading senescence, radiation-induced DSBs (53BP1-foci) dynamics histone variant H2A.J incorporation...