A5069297606- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Protein purification and stability
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Transgenic Plants and Applications
- Virology and Viral Diseases
- Insect Resistance and Genetics
- Animal Virus Infections Studies
- Viral Infections and Outbreaks Research
- Microfluidic and Bio-sensing Technologies
- Microbial Metabolic Engineering and Bioproduction
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- vaccines and immunoinformatics approaches
- Advanced Control Systems Optimization
- thermodynamics and calorimetric analyses
- Biosensors and Analytical Detection
- Extracellular vesicles in disease
McGill University
2016-2025
Siemens (United States)
2021-2023
Siemens Healthcare (United States)
2021-2023
Vrije Universiteit Amsterdam
2023
National Research Council Canada
2011-2022
Université Laval
2000-2021
University of Manitoba
2021
PROTEO
2021
Université de Montréal
2008-2021
Public Health Agency of Canada
2021
BACKGROUND-AIMThe Jaffe method tests for creatinine based on the formation of a colored complex between and alkaline picrate but interfering substances can cause errors.The enzymatic reduces interferences not all labs adopted it due to costs.This study aims create statistical model correct produce results similar those from method, make an easy way adopt daily use. METHODSThree hundred thirty-three (333) patients were enrolled March 20 29, 2019, including walk-in in-patients.Serum values...
Lentiviral vectors (LV) offer several advantages over other gene delivery vectors. Their potential for the integration and long-term expression of therapeutic genes renders them an interesting tool cell therapy interventions. However, large-scale LV production remains important challenge translation LV-based strategies to clinic. The development robust processes mass is needed.A suspension-grown HEK293 line was exploited green fluorescent protein-expressing by transient polyethylenimine...
This article describes the step-wise approach undertaken to select a serum-free medium (SFM) for efficient production of recombinant adenoviral vectors expressing beta-galactosidase (Ad5 CMV-LacZ), in complementing human embryonic kidney 293S cells. In first step, 293S-derived transfectoma, secreting soluble epidermal growth factor receptor sEGFr (D2-22), was used estimate potential selected formulations support protein as compared serum-containing medium. Assays showed that only one among...
Abstract Large‐scale transient transfection of mammalian cells is a recent and powerful technology for the fast production milligram amounts recombinant proteins (r‐proteins). As many r‐proteins used therapeutic structural studies are naturally secreted or engineered to be secreted, cost‐effective serum‐free culture medium that allows their efficient expression purification required. In an attempt design such medium, effect nine protein hydrolysates on cell proliferation, efficiency,...
Abstract The use of lentiviral vectors as gene delivery vehicles has become increasingly popular in recent years. growing interest these created a strong demand for large volumes vector stocks, which entails the need scaleable manufacturing procedures. In this work, we present simple and robust process production using purification methodologies. Lentivirus particles were produced by transient transfection serum‐free suspension‐growing 293 EBNA‐1 cells with four plasmids encoding components...
Abstract Growth, nutrient consumption, and end‐product accumulation were quantitated in shake‐flask cultures of two insect cell lines, Sf‐9 BTI‐EAA, three different serum‐supplemented media. Per consumption or production rates calculated for most medium components analyzed. Glucose was growth‐limiting TNM‐FH the important single source organic‐C cells all cultures. Cells utilized fructose maltose but not sucrose. α‐Ketoglutarate malate contributed significantly to carbon budget TNM‐FH....
Each year, influenza is responsible for hundreds of thousand cases illness and deaths worldwide. Due to the virus' fast mutation rate, World Health Organization (WHO) constantly on alert rapidly respond emerging pandemic strains. Although anti-viral therapies exist, most proficient way stop spread disease through vaccination. The majority vaccines market are produced in embryonic hen's eggs composed purified viral antigens from inactivated whole virus. This manufacturing system, however,...
Vero cells are considered as the most widely accepted continuous cell line by regulatory authorities (such WHO) for manufacture of viral vaccines human use. The growth is anchorage-dependent. Scale-up and manufacturing in adherent cultures labor intensive complicated. Adaptation to grow suspension will simplify subcultivation process scale-up significantly, therefore reduce production cost. Here we report on a successful adaptation serum-free animal component-free medium (IHM03) developed...
We report on the development of surface plasmon resonance (SPR) sensors and matching ELISAs for detection nucleocapsid spike antibodies specific to novel coronavirus 2019 (SARS-CoV-2) in human serum, plasma dried blood spots (DBS).
Utrophin is a close homolog of dystrophin, the protein whose mutations cause Duchenne muscular dystrophy (DMD). present at low levels in normal and dystrophic muscle, whereas dystrophin largely absent DMD. In such cases, replacement using utrophin gene transfer strategy could be more advantageous because would not neoantigen. To establish if adenovirus (AV)-mediated possible option for treatment DMD, an AV vector expressing shortened version (AdCMV-Utr) was constructed. The effect...
Respiration rates in Spodoptera frugiperda (Sf-9) cell bioreactor cultures were successfully measured on-line using two methods: The O2 uptake rate (OUR) was determined gas phase pO2 values imposed by a dissolved oxygen controller and the CO2 evolution (CER) an infrared detector. measurement methods accurate, reliable, relatively inexpensive. CER routinely used for production of several recombinant proteins. Simple linear relationships between viable densities both OUR exponentially growing...
Abstract Gene expression by large‐scale transfection of mammalian cells is becoming an established technology for the fast production milligram and even gram amounts recombinant proteins (r‐proteins). However, efforts are still needed to optimize parameters in order maximize volumetric productivities while maintaining product quality. In this study, efficiency productivity following transient gene HEK293 were evaluated using green fluorescent protein (GFP) human placental secreted alkaline...