A5028485042- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Nanoparticle-Based Drug Delivery
- Cancer-related gene regulation
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Nanoplatforms for cancer theranostics
- Immunotherapy and Immune Responses
- Spine and Intervertebral Disc Pathology
- Graphene and Nanomaterials Applications
- Electrospun Nanofibers in Biomedical Applications
- Cancer Immunotherapy and Biomarkers
- Ferroptosis and cancer prognosis
- Esophageal and GI Pathology
- Pain Mechanisms and Treatments
- Osteoarthritis Treatment and Mechanisms
- Gallbladder and Bile Duct Disorders
- Spinal Cord Injury Research
- Traditional Chinese Medicine Analysis
- Liver physiology and pathology
- Anatomy and Medical Technology
- Mesenchymal stem cell research
- Hydrogen's biological and therapeutic effects
- HVDC Systems and Fault Protection
Hebei Medical University
2024
Second Hospital of Hebei Medical University
2024
Third Xiangya Hospital
2010-2024
Central South University
2010-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Abstract N 6 -methyladenosine (m A) regulators are involved in the progression of various cancers via regulating m A modification. However, potential role and mechanism modification osteosarcoma remains obscure. In this study, WTAP was found to be highly expressed tissue it an independent prognostic factor for overall survival osteosarcoma. Functionally, WTAP, as oncogene, proliferation metastasis vitro vivo. Mechanistically, M dot blot, RNA-seq MeRIP-seq, MeRIP-qRT-PCR luciferase reporter...
Lumbar facet joint osteoarthritis (LFJ OA) is regarded as one of the common causes low back pain (LBP). The pathogenesis and underlying mechanism this disease are largely unknown, there still no effective disease-modifying therapy. This study aims to investigate efficacy exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on behavioral signs LBP in LFJ OA mouse model. pathogenetic change cartilage aberrant nerve invasion subchondral a model after treatment with BMSC-exosomes was...
This study investigated the role of miR-143 in chemoresistance osteosarcoma tumor cells and associated mechanisms. Real-time PCR was used to measure levels. Western blot detect protein expression. Cell proliferation analyzed by MTT assay Matrigel colony formation assay. Forced expression established adenoviral vector infection. death detected Hoechst33342 staining. Loss observed osteosarcomas, which correlated with shorter survival patients osteosarcomas underlying chemotherapy. In...
Abstract Tanshinone I (Tan I) is a widely used diterpene compound derived from the traditional Chinese herb Danshen. Increasing evidence suggests that it exhibits anti‐cancer activity in various human cancers. However, vitro and vivo effects of Tan on osteosarcoma (OS) remain inadequately elucidated, especially those against tumour metastasis. Our results showed significantly inhibited OS cancer cell proliferation, migration invasion induced apoptosis vitro. Moreover, treatment with 10 20...
Chemotherapy is one of the main treatment methods for osteosarcoma. However, conventional chemotherapy lacks targeting properties, and its long-term extensive use will have serious side effects on patients. For this reason, a multifunctional nanodrug system (V-RZCD) osteosarcoma was developed in study. V-RZCD consists two parts: (1) core (ZCD), wherein calcium ions (Ca2+) zoledronic acid (ZA) form metal–organic framework loading doxorubicin (DOX), (2) shell (V-R), vascular endothelial growth...
Tumor necrosis factor (TNF)-α and a disintegrin metalloproteinase with thrombospondin motifs 4 (ADAMTS-4) are important in osteoarthritis (OA) cartilage degradation. In the present study, we explored interaction between two proteins by examining effect of TNF-α on ADAMTS-4 expression activity osteoarthritic chondrocytes. Human chondrocytes were treated different concentrations (5, 15, 30, 45 60 ng/ml) for lengths time (1, 6, 12, 18 24 h) or without TNF receptor 1 (TNFR1) inhibitor SPD304...
Studies have shown that miR-194 functions as a tumour suppressor and is associated with growth metastasis. This study intends to uncover the mechanism of suppression by miR-194. The expression in osteosarcoma cell lines tissues were monitored real-time PCR.The proliferation ability was examined MTT assay. Migration apoptosis cells migration assay flow cytometry, respectively. regulation on CDH2 determined luciferase assays western blot assays.The results showed significantly reduced compared...
Recent studies have demonstrated epigenetic regulation of immune responses. Nevertheless, the underlying effect RNA N6-methyladenosine (m6A) modifications on tumor microenvironment cell infiltration remains elusive. In this study, we thoroughly assessed m6A modification patterns 255 myeloid leukemia specimens based 23 regulators. Consensus clustering regulators was performed to determine three distinct that were remarkably consistent with immunophenotypes tumors: immunorejection, activation,...
Abstract Amphiphilic polymers (HA-ANI) were prepared by grafting hyaluronic acid (HA) and 6-(2-nitroimidazole)hexylamine (ANI) then self-assemble in water to form nanoparticles (NPs) that could be loaded with paclitaxel (PTX) gemcitabine (GEM) dialysis. Infrared spectroscopy 1 H-NMR indicated the successful synthesis of HA-ANI. Three different ratios NPs adjusting hydrophilic hydrophobic materials, particle size decreased as ratio materials increased. When HA:ANI = 2.0:1, had smallest...
Background: Osteosarcoma (OS) is the most common primary tumor of bone in adolescents, and its survival rate generally less than 20% when metastases occur. Necroptosis, a novel form programmed necrotic cell death distinct from apoptosis, has been increasingly recognized as promising therapeutic strategy. This study sought to identify long non-coding RNAs (lncRNAs) associated with necrotizing apoptosis predict prognosis target drug use improve patient survival. Methods: Transcriptomic data...
There are more than 170 subtypes of sarcomas (SARC), which pose a challenge for diagnosis and patient management. Relatively simple or complex karyotypes play an indispensable role in the early effective treatment SARC. The genes related to absorption, distribution, metabolism, excretion (ADME) drug can serve as prognostic biomarkers cancer potential targets. In this study, risk score signature was created. SARC cohort downloaded from Cancer Genome Atlas (TCGA) database, divided into...
Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that would promote the repair spinal cord injury induced by Allen's in rats. At 0.5, 1, 2, 4, 8, 12 24 hours after injury, then once daily 2 weeks, 0.25 mL/kg was infused into subarachnoid space through a catheter. Results at hours, 48 1 week weeks showed markedly reduced cell death, inflammatory...
Implantable electrical nerve stimulation (ENS) can be used to treat neuropathic pain caused by herpes zoster. However, little is known about the cortical mechanism underlying neuromodulation therapy. Here, we recorded a 16-channel resting-state electroencephalogram after application of spinal cord (n = 5) or peripheral 3). The neuromodulatory effect was compared between specific conditions (active ENS versus rest). To capture responses ENS, spectral power and coherence analysis were...
The distal end of the spinal cord and neuromuscular junction may develop secondary degeneration damage following injury because loss neural connections. In this study, a rat model injury, established using modified Allen's method, was injected with basic fibroblast growth factor solution via subarachnoid catheter. After injection, rats displayed higher scores on Basso, Beattie Bresnahan locomotor scale. Motor function also well recovered hematoxylin-eosin staining showed that glial scar...
There is a critical need to developin vitroculture systems appropriate for the expansion of adipose tissue, in order gain new insights into metabolic diseases and assist restoration tissue defects. Conventional two- or three-dimensional (2D 3D)in vitromodels adipocytes require combination supplements induce adipocyte maturation that greatly increases cost large-scale industrial production. In present study, microporous, perforated bacterial cellulose (BC)-assisted culture system was...
N1-methyladenosine methylation (m 1 A), as an important RNA modification, regulates the development of many tumours. Metabolic reprogramming is one features tumour cells, and it plays a crucial role in metastasis. The metabolic osteosarcoma has been widely reported. However, potential roles mechanisms m A-related genes (MRmetabolism) have not currently described. All MRmetabolism were screened, then selected two by least absolute shrinkage selection operator multifactorial regression...
BackgroundRecently, a newly programmed cell death has been discovered, namely cuproptosis. It is considered novel copper-dependent model. Long non-coding RNA (lncRNA) influence the prognosis of bladder cancer. In this study, we established scoring system based on 7 cuproptosis-related lncRNA to predict and immune landscape cancer (BCa).MethodGene expression clinical data 431 tissues were downloaded from The Cancer Genome Atlas (TCGA), including 19 normal samples 419 samples. All randomly...
<title>Abstract</title> <bold>Purpose</bold> This retrospective analysis aimed to assess the feasibility and safety of endoscopic retrograde cholangiopancreatography (ERCP) in pediatric patients by examining ERCP-related adverse events (AEs) occurring over a decade at single center. <bold>Methods</bold> Pediatric under 18 years old who underwent ERCP Second Hospital Hebei Medical University from 1/2013 11/2023 were included. AEs defined according <italic>ERCP-related events: European Society...
We have explored a better method to preserve and store human medically amputated large size samples. The approach involved developing special embalming solution procedures for biopreservation biostorage of large-sized sample as whole specimen rather than dissected small parts. Evaluation the effect our on extremities compared with excised tissues was conducted. Histological morphological techniques elemental analyses were utilized assess effects new using solution.Whole remains (skin,...