A5041727836- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- COVID-19 epidemiological studies
- Viral Infections and Outbreaks Research
- Zoonotic diseases and public health
- Dengue and Mosquito Control Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Streptococcal Infections and Treatments
- Vector-borne infectious diseases
- Vibrio bacteria research studies
- Insect symbiosis and bacterial influences
- Hepatitis Viruses Studies and Epidemiology
- Antibiotic Resistance in Bacteria
- Data-Driven Disease Surveillance
- Long-Term Effects of COVID-19
- Child Nutrition and Water Access
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Hepatitis B Virus Studies
- Bacterial Identification and Susceptibility Testing
- Global Maternal and Child Health
- Virology and Viral Diseases
- Infective Endocarditis Diagnosis and Management
SUNY Upstate Medical University
2015-2024
Coalition for Epidemic Preparedness Innovations
2022-2024
Coalition for Epidemic Preparedness Innovations
2023
Armed Forces Research Institute of Medical Science
2002-2021
International Vaccine Institute
2021
State University of New York
2012-2020
Upstate University Hospital
2012-2020
Universidad Tecnica de Machala
2020
Inter-American Institute
2020
Center for Global Health
2017-2019
Infection with any 1 of 4 dengue viruses produces a spectrum clinical illness ranging from mild undifferentiated febrile to fever (DF) hemorrhagic (DHF), potentially life-threatening disease. The morbidity and mortality DHF can be reduced by early hospitalization careful supportive care. To determine its usefulness as predictor DHF, plasma levels the secreted virus nonstructural protein NS1 (sNS1) were measured daily in 32 children dengue-2 infections participating prospective,...
Dengue hemorrhagic fever (DHF), the most severe form of illness following infection with a dengue virus, is characterized by plasma leakage, thrombocytopenia, and hepatic inflammation. The interrelationships among virus burden, immune activation, development DHF were examined in 54 children secondary dengue-3 infections participating prospective, hospital-based study. was associated higher mean viremia early earlier peak interferon-γ levels. Maximum levels correlated degree leakage...
We investigated an outbreak of multidrug-resistant Acinetobacter baumannii-calcoaceticus complex infection among US service members injured in Iraq.The investigation was conducted Iraq and Kuwait, the 2 military hospitals where majority were initially treated. After characterizing outbreak, we evaluated 3 potential sources for period March 2003 to December 2004. The evaluation included screening samples that obtained from skin patients presence colonization assessing soil health care...
Infection with any 1 of the 4 dengue viruses (DVs) can produce several illnesses, ranging from a mild febrile illness to classic fever (DF) hemorrhagic (DHF), potentially life-threatening disease. Most DHF cases occur after sequential heterotypic DV infections. The role preexisting humoral immunity in modifying severity disease is not well understood.We conducted prospective cohort study children region where hyperendemic and examined neutralizing anti-DV antibodies (Abs) secondary dengue-3...
ABSTRACT The ability of dendritic cells (DCs) to shape the adaptive immune response viral infection is mediated largely by their maturation and activation state as determined surface expression HLA molecules, costimulatory cytokine production. Dengue an emerging arboviral disease where severity illness influenced virus. In this report, we have demonstrated that dengue virus infects replicates in immature human myeloid DCs. Exposure live led both infected surrounding, uninfected DCs...
Abstract: Little is known of the role classical HLA‐A and ‐B class I alleles in determining resistance, susceptibility, or severity acute viral infections. Appropriate paradigms for immunogenetic studies infections are dengue fever (DF) hemorrhagic (DHF). Both primary secondary with virus (DEN) serotypes 1, 2, 3 4, can result either clinically less severe DF more DHF. In exposures, a memory response induced immunologically primed individuals, which both clear infecting contribute to its...
Dengue virus (DENV) infection is a worsening global health problem. The plaque reduction neutralization test (PRNT) currently considered to be the “gold standard” characterize and quantify circulating levels of anti-DENV neutralizing antibody (NAb). Many variations PRNT are in use neither assay nor its performance conditions have been standardized or harmonized between laboratories. We used well-characterized panel acute late convalescent follow-up sera samples from children experiencing...
Background Dengue viruses are a major cause of morbidity in tropical and subtropical regions the world. Inapparent dengue is an important component overall burden infection. It provides source infection for mosquito transmission during course epidemic, yet by definition undetected health care providers. Previous studies inapparent or subclinical have reported varying ratios symptomatic to Methodology/Principal Findings In prospective study school children Northern Thailand, we describe...
Background. Despite the strong association between secondary dengue virus (DENV) infections and hemorrhagic fever (DHF), majority of are subclinical or mild. The determinants clinical severity remain unclear, though studies indicate a titer-dependent time-dependent role cross-protective anti-DENV antibodies.
Dengue fever and dengue hemorrhagic constitute a substantial health burden on the population in Thailand. In this study, impact of symptomatic virus infection families patients hospitalized at Kamphaeng Phet Provincial Hospital with laboratory-confirmed 2001 was assessed, disability-adjusted life years (DALYs) lost for fatal non-fatal cases were calculated using level data When we accounted direct cost hospitalization, indirect costs due to loss productivity, average number persons infected...
ABSTRACT Members of the genus Acinetobacter are ubiquitous in soil and water an important cause nosocomial infections. A rapid method is needed to genotype isolates determine epidemiology clonality during infectious outbreaks. Multilocus PCR followed by electrospray ionization mass spectrometry (PCR/ESI-MS) a that uses amplicon base compositions bacterial species. In order identify regions genome useful for this method, we sequenced six housekeeping genes ( trpE , adk efp mutY fumC ppa )...
A fundamental mystery for dengue and other infectious pathogens is how observed patterns of cases relate to actual chains individual transmission events. These pathways are intimately tied the mechanisms by which strains interact compete across spatial scales. Phylogeographic methods have been used characterize pathogen dispersal at global regional scales but yielded few insights into local spatiotemporal structure endemic transmission. Using geolocated genotype (800 cases) serotype (17,291...
It is well-known that the distribution of immunity in a population dictates future incidence infectious disease, but this process generally understood at individual or macroscales. For example, herd to multiple pathogens has been observed national and city levels. However, effects have not previously shown scales smaller than (e.g., neighborhoods). In particular, no study long-term consistent with spatial scale person-to-person transmission. Here, we use location dengue patients' homes...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of pandemic human illness COVID-19, is a global health emergency. While severe disease has been linked to an expansion antibody-secreting plasmablasts, we sought identify B cell responses that correlated with positive clinical outcomes in convalescent patients. We characterized peripheral blood immunophenotype and plasma antibody 40 recovered non-hospitalized COVID-19 subjects were enrolled as donors treatment...
ABSTRACT Some individuals infected with dengue virus develop hemorrhagic fever (DHF), a viral disease characterized by transient period of localized plasma leakage. To determine the importance vascular endothelial growth factor A (VEGF-A) in this syndrome, we compared levels VEGF-A and soluble forms its receptors patients DHF to (DF), milder form infection without We observed rise free, but not total at time This was associated decline VEGF receptor 2 (VEGFR2) VEGF-soluble VEGFR2 complexes,...
Children who experience secondary dengue virus (DV) infections are at increased risk for hemorrhagic fever. To study the effect of preexisting T cell responses to DV on severity infection, peripheral blood mononuclear cells (PBMC) from 10 subsequently hospitalized and 12 nonhospitalized Thai schoolchildren were stimulated with inactivated antigens, proliferation interferon (IFN)-γ or tumor necrosis factor (TNF)-α preinfection PBMC measured. Proliferation observed in 11 subjects, IFN-γ seen 6...
Background. The understanding of dengue virus (DENV) transmission dynamics and the clinical spectrum infection are critical to informing surveillance control measures. Geographic cluster studies can elucidate these features in greater detail than cohort alone. Methods. A 4-year longitudinal geographic study was undertaken rural Thailand. Cohort children underwent pre-/postseason serology active school absence–based detect inapparent symptomatic dengue. Cluster investigations were triggered...
Dengue viruses (DENVs) and Japanese encephalitis virus (JEV) have significant cross-reactivity in serological assays; the clinical implications of this remain undefined. An improved understanding whether how JEV immunity modulates outcome DENV infection is important as large-scale vaccine trials will commence areas where co-endemic and/or immunization routine.The association between preexisting neutralizing antibodies (NAbs) severity was evaluated a prospective school-based cohort Thailand...