A5007056143- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Analytical Chemistry and Chromatography
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Birth, Development, and Health
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Pregnancy and preeclampsia studies
- Enzyme Structure and Function
- Metabolomics and Mass Spectrometry Studies
- vaccines and immunoinformatics approaches
- Ubiquitin and proteasome pathways
- Bacterial Genetics and Biotechnology
- Genetics, Aging, and Longevity in Model Organisms
- Gestational Diabetes Research and Management
- Synthesis and Reactions of Organic Compounds
- Ion-surface interactions and analysis
- Molecular Biology Techniques and Applications
- Synthesis and Catalytic Reactions
- Immunotherapy and Immune Responses
- Rheumatoid Arthritis Research and Therapies
- Hemoglobin structure and function
- Bacteriophages and microbial interactions
University of Rostock
2016-2025
Huazhong University of Science and Technology
2014
Wuhan National Laboratory for Optoelectronics
2014
Shanghai Academy of Science & Technology
2014
Proteome Sciences (United Kingdom)
2006
University of Konstanz
1991-2003
Institute of Immunology
2003
Oregon State University
1993-1994
Abstract The development of “soft” ionization methods in recent years has enabled substantial progress the mass spectrometric characterization macromolecules, particular important biopolymers such as proteins and nucleic acids. In contrast to still existing limitations for determination molecular weights by other fast atom bombardment plasma desorption, electrospray (ESI) matrix‐assisted laser desorption have provided a breakthrough macromolecules larger than 100 kDa. Whereas these been...
Differential proteome analysis is used to study body fluids from patients suffering rheumatoid arthritis (RA), reactive (reaA) or osteoarthritis (OA). Mass spectrometric structure characterization of gel-separated proteins provided a detailed view the protein-processing events that lead distinct protein species present in respective fluids. (i) Fibrin(ogen) β-chain degradation products, presumably plasmin-derived, appeared solely synovial (SF) both patient collectives, (ii) calgranulin B...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular Characterization of Surface Topology in Protein Tertiary Structures by Amino-Acylation and Mass Spectrometric Peptide MappingMichael O. Glocker, Christoph Borchers, Winfried Fiedler, Detlev Suckau, Michael PrzybylskiCite this: Bioconjugate Chem. 1994, 5, 6, 583–590Publication Date (Print):November 1, 1994Publication History Published online1 May 2002Published inissue 1 November...
Post-translational modifications (PTMs) of proteins play essential roles in almost all cellular processes, and are closely related to physiological activity disease development living organisms. The tandem mass spectrometry (MS/MS) has resulted a rapid increase PTMs identified on from different species. collection systematic ordering PTM data should provide invaluable information for understanding processes signaling pathways regulated by PTMs. For this original purpose we developed SysPTM,...
The heat shock protein Hsp33 is a very potent molecular chaperone with distinctive mode of functional regulation; its activity redox-regulated. In reduced form all six cysteinyl residues are present as thiols, and displays no folding helper activity. Exposure to oxidizing conditions like H<sub>2</sub>O<sub>2</sub>, however, rapidly converts into an efficient chaperone. Activated binds tightly refolding intermediates chemically denatured luciferase suppresses efficiently their aggregation...
While electrospray (ESI) mass spectrometry has already established its potential for the characterization of non-covalent protein complexes, matrix-assisted laser desorption/ionization (MALDI/MS) seemed not to be applicable hitherto because limitations in matrix chemistry and sample preparation. In this work, a preparation method been developed which 6-aza-2-thiothymine (ATT) was used as without any addition organic cosolvents, proteins were dissolved aqueous buffers such ammonium...
Many Streptococcus pyogenes (group A streptococcus [GAS]) virulence factor- and transcriptional regulator-encoding genes cluster together in discrete genomic regions. Nra is a central regulator of the FCT region. Previous studies exclusively described as repressor adhesin toxin genes. Here transcriptome proteome analysis serotype M49 GAS strain an isogenic mutant this revealed complete regulon profile. active all growth phases tested, with largest transition phase. Almost exclusively,...
Parkinson's disease is a multifactorial, neurodegenerative where etiopathogenetic mechanisms are not fully understood. Animal models like the neurotoxic 6-OHDA-hemiparkinsonian rat model used for standardized experiments. Here, we analyzed proteome changes of striatum three months after 6-OHDA lesions nigral dopaminergic cell population. Striata were removed and proteins separated by 2DE followed differential spot analysis. Proteins in spots identified MALDI-TOF-MS. Most up-regulations...
Abstract Two‐dimensional gel electrophoresis‐separated and excised haptoglobin α2‐chain protein spots were subjected to in‐gel digestion with trypsin. Previously unassigned peptide ion signals observed in mass spectrometric fingerprinting experiments sequenced using the matrix‐assisted laser desorption/ionization‐quadrupole trap‐time of flight (MALDI‐QIT‐TOF) spectrometer showed that α‐chain derivative under study was cleaved by trypsin unspecifically. Abundant cleavages occurred C ‐terminal...
Abstract Complement receptor type II (CR2/CD21) is the major for C3d fragments on immune complexes. CD21 also serves as Epstein–Barr virus in humans. On mature B cells, reduces threshold of BCR signaling together with CD81, Leu13 and CD19, but it occurs other cells system where performs unknown functions. A soluble form (sCD21) shed from cell surface found human blood plasma. An as‐yet‐unknown protease thought to be responsible this shedding. Altered levels sCD21 occur plasma certain...
To investigate the serum level distribution of angiogenic markers (PlGF, endoglin, sFlt-1) and acute-phase proteins (SAA, CRP) in patients with HELLP syndrome or preeclampsia (PE) including matched controls.The matching procedure revealed 46 controls for 23 cases, 81 42 preeclamptic patients. Maternal concentrations were determined by immunoassays.SAA CRP levels significantly higher compared controls. This finding was not observed subgroup. Pro-angiogenic PlGF is lower PE syndrome....
Intact transition epitope mapping (ITEM) enables rapid and accurate determination of protein antigen-derived epitopes by either extraction or excision. Upon formation the antigen peptide-containing immune complex in solution, entire mixture is electrosprayed to translate all constituents as protonated ions into gas phase. There, from antibody–peptide complexes are separated unbound peptide according their masses, charges, shapes ion mobility drift quadrupole filtering. Subsequently,...
Origanum L. (Lamiaceae) is an important genus of medicinal and aromatic plants used in traditional medicine since ancient times as culinary herbs remedies. The aim the present study was to evaluate chemical composition, well biochemical cellular activities freshly prepared majorana essential oil (OmEO) Alzheimer's disease (AD) amyloid beta1-42 (Aβ1-42) rat model. OmEO (1% 3%) inhaled for 21 consecutive days, while Aβ1-42 administered intracerebroventricularly induce AD-like symptoms. Our...
Immunoassays for cardiac troponin, such as the Elecsys&reg; hs-TnT, have become gold standard myocardial infarction diagnostics. While various protein/chemical factors affecting troponin complex and, thus, its diagnostic accuracy been investigated, role of coding single nucleotide polymorphisms remains underexplored. To evaluate potential cSNP-induced interference with antibody binding in hs-TnT immunoassay, we applied ITEM-FOUR, a mass spectrometry-based method that quantifies changes...
Immunoassays for cardiac troponin, such as the Elecsys® hs-TnT, have become gold standard myocardial infarction diagnostics. While various protein/chemical factors affecting troponin complex and thus its diagnostic accuracy been investigated, role of coding single-nucleotide polymorphisms remains underexplored. To evaluate potential cSNP-induced interference with antibody binding in hs-TnT immunoassay, we applied ITEM-FOUR, a mass spectrometry-based method that quantifies changes upon amino...
Abstract Die Entwicklung von schonenden Ionisierungsmethoden hat in den letzten Jahren zu bedeutenden Fortschritten der massenspektrometrischen Charakterisierung Makromolekülen, insbesondere Biopolymeren wie Proteinen und Nucleinsäuren, geführt. Während bei anderen Methoden dem Beschuß mit schnellen Atomen (Fast Atom Bombardment, FAB) Plasmadesorption noch Molekulargewichtsbegrenzungen bestehen, gelang Hilfe Elektrospray‐ (ESI‐MS) matrixunterstützten...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAssignment of the inter- and intramolecular disulfide linkages in recombinant human macrophage colony stimulating factor using fast atom bombardment mass spectrometryMichael O. Glocker, Brian Arbogast, Jolanda Schreurs, Max L. DeinzerCite this: Biochemistry 1993, 32, 2, 482–488Publication Date (Print):January 19, 1993Publication History Published online1 May 2002Published inissue 19 January...
Haptoglobin belongs to the major constituents of plasma and acts as hemoglobin-binding acute-phase protein. Due occurrence three allelic variants further structural modifications, alpha chains haptoglobin form varying spot patterns in two-dimensional gel electrophoresis (2-DE) gels, which is generally observed differential proteome analyses using or related body fluids humans. In present study samples from 10 donors initially unknown phenotype were separated by 2-DE tryptic digests excised...