A5007599325- Venomous Animal Envenomation and Studies
- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Biochemical and Structural Characterization
- Blood Coagulation and Thrombosis Mechanisms
- Blood properties and coagulation
- Protease and Inhibitor Mechanisms
- Antiplatelet Therapy and Cardiovascular Diseases
- Angiogenesis and VEGF in Cancer
- Healthcare and Venom Research
- Blood disorders and treatments
- Cardiac electrophysiology and arrhythmias
- Erythrocyte Function and Pathophysiology
- Neuroscience of respiration and sleep
- Traditional and Medicinal Uses of Annonaceae
- Inflammatory mediators and NSAID effects
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Cancer, Hypoxia, and Metabolism
- Cardiac Ischemia and Reperfusion
- Heparin-Induced Thrombocytopenia and Thrombosis
- Antimicrobial Peptides and Activities
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Nitric Oxide and Endothelin Effects
- Phytochemistry and Biological Activities
National Taiwan University
2014-2025
Mackay Medical College
2017-2025
Zhejiang Normal University
2025
Affiliated Hospital of Nantong University
2024
Nantong University
2024
National Defense Medical Center
1994-2015
Tri-Service General Hospital
2015
Abraxis (United States)
2009
National Yang Ming Chiao Tung University
1999
Chang Gung University
1998
Platelet-leukocyte interactions amplify inflammatory reactions, but the underlying mechanism is still unclear. CLEC5A and CLEC2 are spleen tyrosine kinase (Syk)-coupled C-type lectin receptors, abundantly expressed by leukocytes platelets, respectively. Whereas a pattern recognition receptor (PRR) to flaviviruses bacteria, for platelet-activating snake venom aggretin. Here we show that dengue virus (DV) activates platelets via release extracellular vesicles (EVs), including exosomes (EXOs)...
Abstract Trigramin, a highly specific inhibitor of fibrinogen binding to platelet receptors, was purified homogeneity from Trimeresurus gramineus snake venom. Trigramin is single chain (approximately 9 kDa) cysteine-rich peptide with the Glu-Ala-Gly-Glu-Asp-Cys-Asp-Cys-Gly-Ser-Pro-Ala NH2-terminal sequence. Chymotryptic fragmentation showed Arg-Gly-Asp sequence in trigramin. inhibited fibrinogen-induced aggregation platelets stimulated by ADP (IC50 = 1.3 X 10(-7)M) and chymotrypsin-treated...
It has been shown that ultrasound (US) stimulation accelerates fracture healing in animal models and clinical studies. Here we found US transiently increased the surface expression of α2, α5, β1, β3 integrins cultured osteoblasts, as by flow cytometric analysis immunofluorescence staining. prostaglandin E<sub>2</sub> formation protein mRNA levels cyclooxygenase-2 (COX-2). At mechanistic level, anti-integrin α5β1 αvβ3 antibodies or rhodostomin, a snake venom disintegrin, attenuated US-induced...
Trigramin, a naturally occurring peptide purified from Trimeresurus gramineus (T. stejnegeri formosensis) snake venom, inhibits platelet aggregation and the binding of 125I-fibrinogen to ADP-stimulated platelets (Ki = 2 X 10(-8) M) without affecting platelet-release reaction. 125I-trigramin binds chymotrypsin-treated normal but not thrombasthenic platelets. is blocked by monoclonal antibodies directed against glycoprotein IIb/IIIa complex Arg-Gly-Asp-Ser (RGDS) [Huang et al. (1987) J. Biol....
Abstract Cardiovascular disease (CVD) is strongly associated with the gut microbiota and its metabolites, including trimethylamine- N -oxide (TMAO), formed from metaorganismal metabolism of ʟ-carnitine. Raw garlic juice, allicin as primary compound, exhibits considerable effects on microbiota. This study validated benefits raw juice against CVD risk via modulation metabolites. Allicin supplementation significantly decreased serum TMAO in ʟ-carnitine-fed C57BL/6 J mice, reduced aortic...
Podoplanin (PDPN) enhances tumor metastases by eliciting cell-induced platelet aggregation (TCIPA) through activation of C-type lectin-like receptor 2 (CLEC-2). A novel and non-cytotoxic 5-nitrobenzoate compound 2CP was synthesized that specifically inhibited the PDPN/CLEC-2 interaction TCIPA with no effect on stimulated other agonists. possessed anti-cancer metastatic activity in vivo augmented therapeutic efficacy cisplatin experimental animal model without causing a bleeding risk....
Angiogenesis consists of the proliferation, migration, and differentiation endothelial cells, although angiogenic factor integrin-extracellular matrix interaction modulate this process. We report here that a snake venom-derived disintegrin, rhodostomin, inhibited distinct steps in angiogenesis elicited by basic fibroblast growth (bFGF), also suppressed vivo murine melanoma tumor growth. Rhodostomin dose-dependently bFGF-induced human umbilical vein cell (HUVEC) proliferation as examined...
A potent platelet aggregation inducer, aggretin, was purified from Malayan-pit-viper (Calloselasma rhodostoma) venom by ionic-exchange chromatography, gel-filtration chromatography and HPLC. It is a heterodimeric protein (29 kDa) devoid of esterase, phospholipase thrombin-like activity. Aggretin (> 5 nM) elicited with lag period in both human platelet-rich plasma washed suspension. EDTA (5 mM), prostaglandin E1 (1 microM) 3,4,5-trimethoxybenzoic acid 8-(diethylamino)octyl ester ('TMB-8'; 100...