A5087717884- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Ovarian function and disorders
- Birth, Development, and Health
- Neuroendocrine regulation and behavior
- Reproductive Health and Technologies
- Stress Responses and Cortisol
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- Seed Germination and Physiology
- RNA Interference and Gene Delivery
- Assisted Reproductive Technology and Twin Pregnancy
- Enzyme function and inhibition
- Reproductive biology and impacts on aquatic species
- Male Reproductive Health Studies
- Advanced Fluorescence Microscopy Techniques
- Reproductive System and Pregnancy
- Chemical Reactions and Mechanisms
- Cholinesterase and Neurodegenerative Diseases
- Plant Reproductive Biology
Universidad Nacional Autónoma de México
2013-2024
Sperm capacitation is a complex and indispensable physiological process that spermatozoa must undergo in order to acquire fertilization capability. Spermatozoa from several mammalian species, including mice, exhibit capacitation-associated plasma membrane hyperpolarization, which necessary for the acrosome reaction occur. Despite its importance, this hyperpolarization event has not been adequately examined human sperm. In report we used flow cytometry show subpopulation of sperm indeed upon...
Mammalian sperm require to spend a limited period of time in the female reproductive tract become competent fertilize process called capacitation. It is well established that HCO3- essential for capacitation because it activates atypical soluble adenylate cyclase ADCY10 leading cAMP production, and promotes alkalinization cytoplasm, membrane hyperpolarization. However, how transported into not understood. There evidence CFTR activity involved human but this channel integrated complex...
The sperm acrosome reaction (AR), an essential event for mammalian fertilization, involves Ca 2+ permeability changes leading to exocytosis of the acrosomal vesicle. acrosome, intracellular store whose luminal pH is acidic, contains hydrolytic enzymes. It known that (pH acr ) increases during capacitation and this correlates with spontaneous AR. Some AR inducers increase concentration ([Ca ] i through release from internal stores, mainly acrosome. Catsper, a specific channel, has been...
Is image-based flow cytometry a useful tool to study intracellular events in human sperm such as protein tyrosine phosphorylation or signaling processes?Image-based is powerful relevant number of cells, which enables robust statistical analysis providing spatial resolution terms the specific subcellular localization labeling.Sperm capacitation required for fertilization. During this process, spermatozoa undergo numerous physiological changes, via activation different pathways, are not...
Spermatozoa are male reproductive cells especially designed to reach, recognize and fuse with the egg. To perform these tasks, sperm must be prepared face a constantly changing environment overcome several physical barriers. Being in essence transcriptionally translationally silent, motile rely profoundly on diverse signaling mechanisms orient themselves swim directed fashion, contend challenging environmental conditions during their journey find In particular, Ca2+-mediated is pivotal for...
In brief Hyperpolarization of the membrane potential is a crucial step for mammalian sperm maturation. This work demonstrates that this change likely activates sperm-specific sodium/proton exchanger to induce alkalization in mouse flagellum. Abstract The (sNHE) an indispensable protein male fertility mammals. Nevertheless, it still unknown how sNHE regulated. Evidence obtained from sea urchin indicates hyperpolarization plasma (Vm), which hallmark capacitation, positively regulates sNHE....
Filamentous actin (F-actin) is a key factor in exocytosis many cell types. In mammalian sperm, acrosomal (denoted the acrosome reaction or AR), special type of controlled secretion, regulated by multiple signaling pathways and cytoskeleton. However, dynamic changes cytoskeleton live sperm are largely not understood. Here, we used powerful properties SiR-actin to examine dynamics mouse at onset AR. By using combination super-resolution microscopy techniques image loaded with from transgenic...
Sperm capacitation, crucial for fertilization, occurs in the female reproductive tract and can be replicated
Plasma membrane (PM) hyperpolarization, increased intracellular pH (pHi), and changes in calcium concentration ([Ca2+]i) are physiological events that occur during human sperm capacitation. These parameters potential predictors of successful outcomes for men undergoing artificial reproduction techniques (ARTs), but methods currently available their determination pose various technical challenges limitations. Here, we developed a novel strategy employing time-lapse flow cytometry (TLFC) to...
The objective of this study was to evaluate the effects caffeine and taurine on motility viability chilled equine semen.A total 12 ejaculates were collected from three mature stallions with proven fertility during breeding season. gel-free spermatic fraction each ejaculate divided into two aliquots diluted a semen extender (either INRA 96® or BotuSemen Gold®). then split assigned one six treatment groups: control (no supplement), (2 4 mM), (25 50 combination mM) plus mM). Samples stored at...
Abstract Study question Does the change in intracellular calcium levels evoked by artificial membrane depolarizations predict fertilization success a non-normozoospermic semen sample undergoing assisted reproductive techniques? Summary answer The increase after plasma depolarization correlates negatively with rates conventional IVF but not ICSI What is known already Sperm capacitation-related features, like regulation of concentration ([Ca2+]i) and hyperpolarization potential (Vm), have been...
Abstract Study question Does measuring qualitative changes in the sperm pHi could predict fertilizing potential of a non-normozoospermic patients under fertility treatment? Summary answer The increase correlates with fertilization rates. What is known already intracellular (pHi) great importance for wide variety physiological processes, including its motility. regulates several key proteins, such as Ca2+ channel, CatSper (sperm cation channel) which involved hyperactivation and K+ channel...
The cryopreservation of spermatozoa has the main purpose preserving male fertility. However, current preservation techniques have shown to produce lesions in structure and alter sperm functions, probably due production reactive oxygen species (ROS) during cryopreservation. To overcome damage provoked by ROS, we introduced a novel antioxidant called EmbryORP® vitrification protocol compared eight fertility parameters: motility, viability, morphology, concentration, semen pH,...
Spermatozoa are male reproductive cells especially designed to reach, recognize and fuse with the egg. To perform these tasks, sperm must be prepared face a constantly changing environment overcome several physical barriers. Being in essence transcriptionally translationally silent, motile rely profoundly on diverse signaling mechanisms orient themselves swim directed fashion, contend challenging environmental conditions during their journey find In particular, Ca2+-mediated is pivotal for...
Abstract Study question Can the overall intracellular Ca2+ dynamics evoked by progesterone stimulation predict fertilizing potential of a non-normozoospermic semen sample under fertility treatment? Summary answer Some kinetic parameters transient increase correlate with fertilization rates in conventional IVF but not ICSI. What is known already Stimulation female hormone induces activation sperm-specific ion channel CatSper which turn produces fast concentration ([Ca2+]i). After reaching...