Over the last decades, endophytic fungi represent a new source of pharmacologically active secondary metabolites based on underlying assumption that they live symbiotically within their plant host. In present study, fungus was isolated from Rauwolfia macrophylla, medicinal Cameroon. The showed highest homology to Curvularia sp. complete nucleotide sequence data generated internal transcribed spacer (ITS) ribosomal DNA region. Large scale fermentation, working-up and separation strain extract...

During our search for Streptomyces spp. as new producers of bioactive secondary metabolites, the ethyl acetate extract terrestrial isolate TN262 delivered eight antimicrobially active compounds. They were identified 1-acetyl-β-carboline (1), tryptophol (2), cineromycin B (3), 2,3-dihydrocineromycin (4), cyclo-(tyrosylprolyl) (5), 3-(hydroxyacetyl)-indole (6), brevianamide F (7), and cis-cyclo-(l-prolyl-l-leucyl) (8). Three further metabolites detected in unpolar fractions using GC–MS...

Chemical screening of the ethyl acetate extract from marine-derived Streptomyces sp. isolate Mei37 resulted in five isoquinolinequinones, four new derivatives, mansouramycin A−D (1, 3−5), and known 3-methyl-7-(methylamino)-5,8-isoquinolinedione (2). Their structures were elucidated by NMR MS techniques comparison with related compounds. Cytotoxicity profiling mansouramycins a panel up to 36 tumor cell lines indicated significant cytotoxicity several pronounced selectivity for non-small lung...

Abstract The chemical constituents and biological activities of the terrestrial Aspergillus flavipes MM2 isolated from Egyptian rice hulls are reported. Seven bioactive compounds were obtained, which one sterol: ergosterol ( 1 ), four butyrolactones: butyrolactone I 2 aspulvinone H 3 butyrolactone-V 6 ) 4,4'-diydroxypulvinone 7 along with 6-methylsalicylic acid 4 cyclopentenone analogue; terrien 5 ). Structures deduced by intensive studies their 1D & 2D NMR, MS data comparison related...

The chemical composition and biological activity of three parts (rind, flesh seeds) pumpkin fruits (Cucurbita pepo L.) cultivated in Egypt were studied. Chemical analysis fibre, protein, β-carotene, carbohydrates, minerals fatty acids present the rind, flesh, seeds defatted meal was conducted. Chemical, GC-MS assays organic extracts main fruit parts, rind established their unique constituents. Chromatographic purification afforded triglyceride acid mixture (1), tetrahydro-thiophene (2),...

Eight bioactive pyrone derivatives were identified from the culture of Alternaria alternata strain D2006, isolated marine soft coral Denderonephthya hemprichi, which was selected as its profound antimicrobial activities. The compounds assigned pyrophen (1), rubrofusarin B (2), fonsecin (3), and (5) beside to four dimeric naphtho-γ-pyrones; aurasperone A (6), (7), C (8), F (9). Structures on basis 1D 2D NMR spectroscopy mass (EI, ESI, HRESI) data, by comparison with literature. Configuration...

The crystal structure and unambiguous absolute configuration of meleagrin (1) isolated from fungus Emericella dentata Nq45 is reported herein to first time on the bases single X-ray diffraction. Together with 1, haenamindole (2), isorugulosuvine (3), secalonic acid D (4), ergosterol (5) cerebroside A (6) were obtained their structures determined by ESI MS NMR data analysis. Diverse biological activity was investigated. Compound 1 pronounced potent cytotoxicity against human cervix carcinoma...

From the ethyl acetate extract of strain Streptomyces sp. isolate GW23/1540, besides 16 known products, several 1H-quinazolin-4-one derivatives were isolated. (S*R*)-2-(1-Hydroxyethyl)-2-methyl-2,3-dihydro-1H-quinazolin-4-one (4) and (R*R*)-2-(1-hydroxyethyl)-2-methyl-2,3-dihydro-1H-quinazolin-4-one (5) are new natural products. 2-Methyl-3H-quinazolin-4-one (2) 1H-quinazoline-2,4-dione (3) from other bacteria plants, respectively. another sp., GW2/577, 5-methyl-1H-quinazoline-2,4-dione (6)...

The chemical constituents and biological activities of leaves roots Aloe hijazensis, collected in Saudi Arabia, are reported here for the first time. Twenty-two compounds were obtained, among them eight hydroxyquinones: aloe-emodin (1), emodin (2), chrysophanol (3), aloesaponarin II 3-methyl ether (4), ziganein (5), ziganein-5-methyl (6a), I (7) chrysophanein (8), dihydro-isocoumarin feralolide (9), 4,7-dichloro-quinoline (10), triterpene lupeol (11), anthrone aloin (12), three aloenin...

Terretonin N (1), a new highly oxygenated and unique tetracyclic 6-hydroxymeroterpenoid, was isolated together with seven known compounds from the ethyl acetate extract of solid-state fermented culture Nocardiopsis sp. Their structures were elucidated by spectroscopic analysis. The structure absolute configuration 1 unambiguously determined X-ray crystallography. isolation taxonomic characterization is reported. antimicrobial activity cytotoxicity strain compound studied using different...

The anticancer activity of terretonin N (1) and butyrolactone I (2), obtained from the thermophilic fungus Aspergillus terreus TM8, was intensively studied against prostate adenocarcinoma (PC-3) ovary (SKOV3) human cell lines. According to this study, both compounds showed potent cytotoxicity towards ovarian cells with IC50 1.2 0.6 μg/mL, respectively. With respect metastatic (PC-3), two 1 2 a significantly promising effect 7.4 4.5 tested fungal metabolites higher rates early late apoptosis...

More than 15,000 marine products have been described up to now; Sponges are champion producers, concerning the diversity of that found. Most bioactive compounds from sponges were classified into anti-inflammatory, antitumor, immuno- or neurosurpressive, antiviral, antimalarial, antibiotic, antifouling. Evaluation in vitro inhibitory effects different extracts four versus some antioxidants indices and carbohydrate hydrolyzing enzymes concerned with diabetes mellitus was studied. The chemical...

In our attempt to explore bioactive metabolites from marine fungi having the potential overcome Helicobacter pylori, five compounds of sesquiterpenoid and alkaloid nature, have been evaluated. They were structurally assigned as aspterric acid (1), aurantiomide C (2), 4-(2-hydroxyt-3-butynoxy) benzoic (3), aspermytin A (4) cyclopenol (5). The isolated Sarcophyton-derived Penicillium sp. identified recently by us. Biologically, all showed activity against H. pylori; their effectiveness ranged...

In this study, extracts of the flowers folk medicinal plant Bombax malabaricum DC were biologically and chemically screened. Chemical constituents in n-hexane fraction from B. investigated using gas-liquid chromatography (GLC) analysis, affording 14 compounds, including cholesterol, stigmasterol, campesterol α-amyrin, while residual 10 compounds are hydrocarbons. GLC analysis fatty acid (FA) esters established majority abundance saturated FA over their unsaturated analogues. The polar...

Two new diterpenoids, pachydictyol B (1a/1b) and C (2), were isolated from the dichloromethane extract of marine brown alga, Dictyota dichotoma, collected Red Sea coast Egypt, along with known metabolites, A (3a), dictyol E (4), cis-africanan-1α-ol (5a), fucosterol (6), tetrahydrothiophen-1,1-dioxide poly-β-hydroxybutyric acid. GC-MS analysis nonpolar fractions also indicated presence β-bourbonene nonanal, three hydrocarbons five fatty acids or their simple derivatives, respectively....

Terretonin M (1), a new highly oxygenated tetracyclic meroterpenoid, was isolated from the thermophilic fungus Aspergillus terreus TM8 together with 10 known metabolites: terrelumamide A, asterrelenin, 7-prenyl-indolyl-3-carbaldehyde, (3β,5α,6β)-3,5,6-trihydroxy-ergosta-7,22-diene, sitostenone, linoleic acid, ergosterol, uracil, p-hydroxy-benzoic and indole-3-carboxylic acid. The chemical structure of compound elucidated by extensive 1D, 2D NMR, ESI HR mass measurements, comparison...

Isoshamixanthone (1), a new stereoisomeric pyrano xanthone together with the previously known fungal metabolites, epiisoshamixanthone (2), sterigmatocystin (3), arugosin C (4), norlichexanthone (5), diorcinol (6), ergosterol and methyllinoleate, were obtained from endophytic strain Aspergillus sp. ASCLA isolated leaf tissues of medicinal plant Callistemon subulatus. The chemical structure (1) was elucidated by extensive 1D, 2D NMR, ESI HR mass measurements, comparison literature data....

Antibacterial and cytotoxic activities of Euphorbia balsamifera, fractions pure compounds were evaluated. The assays for HCT116, HePG2 MCF7 showed a significant IC50: 54.7 76.2 µg/mL non-polar fraction "n-hexane" against HCT116 HePG2, respectively. results revealed that plant exhibited potential the tested pathogens than total extract where n-butanol ethyl acetate antibacterial activity (P < 0.05) bacterial strains. Isolation structure determination from n-hexane performed. From fraction,...

The anticancer, antimicrobial, and insecticidal activities of sarcotrocheliol (1) cholesterol (2) obtained from the soft coral Sarcophyton trocheliophorum (S. trocheliophorum) were intensively studied. According to this study, both compounds 1 2 showed potential cytotoxicity towards human colorectal carcinomaHCT-116 (IC50 10.4, 11.8 µg/mL) liver carcinoma HepG2 cell lines 8.8, 12.0 µg/mL), respectively. Compounds evaluated as inhibitors caspase-3, a member cysteine protease family, which is...