A5065696488- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Trace Elements in Health
- Iron Metabolism and Disorders
- Biotin and Related Studies
- Catalytic C–H Functionalization Methods
- Photosynthetic Processes and Mechanisms
- Genetic and phenotypic traits in livestock
- Vitamin D Research Studies
- Hemoglobinopathies and Related Disorders
- Antibiotics Pharmacokinetics and Efficacy
- Advanced Photocatalysis Techniques
- Advanced Nanomaterials in Catalysis
- Nanocluster Synthesis and Applications
- Plant Micronutrient Interactions and Effects
- Genetic Associations and Epidemiology
- Zebrafish Biomedical Research Applications
- Redox biology and oxidative stress
- Advanced biosensing and bioanalysis techniques
- Hematopoietic Stem Cell Transplantation
- Enzyme Catalysis and Immobilization
- Carbohydrate Chemistry and Synthesis
- Vitamin C and Antioxidants Research
- Asymmetric Hydrogenation and Catalysis
- Pluripotent Stem Cells Research
Anhui University
2024
University of Florida
2017-2023
Shanghai Jiao Tong University
2021
University of Nebraska–Lincoln
2003-2014
Durham University
2003
The molecular basis by which organisms detect and respond to fluctuations in inorganic carbon is not known. cyaB1 gene of the cyanobacterium Anabaena sp. PCC7120 codes for a multidomain protein with C-terminal class III adenylyl cyclase catalyst that was specifically stimulated bicarbonate ion (EC50 9.6 mm). Bicarbonate lowered substrate affinity but increased reaction velocity. A point mutation active site (Lys-646) reduced activity 95% refractory activation. We propose Lys-646 coordinates...
Iron homeostasis is tightly regulated, and the peptide hormone hepcidin considered to be a principal regulator of iron metabolism. Previous studies in limited number mouse strains found equivocal sex- strain-dependent differences mRNA serum levels reported conflicting data on relationship between (Hamp1) status. Our aim was clarify relationships strain, sex, expression by examining multiple tissues effects different dietary conditions inbred strains. Two were done: first, Hamp1 mRNA, liver...
In a recent mass spectrometry screen, we identified 108 new proteins that were modified endogenously by covalent binding of biotin; members the heat shock superfamily proteins, including protein 72 (HSP72), overrepresented among biotinylated proteins. Mammals respond to infections secreting extracellular HSP72 (eHSP72), which elicits an immune response. Here, using and site-directed mutagenesis, five biotinylation sites in HSP72. We used coimmunoprecipitation, spectrometry, limited...
Abstract Transferrin‐bound iron (TBI), the physiological circulating form, is acquired by cells through transferrin receptor (TfR1) endocytosis. In erythroid cells, most of incorporated into heme in mitochondria. Cellular trafficking indispensable for erythropoiesis and many other essential biological processes. Comprehensive elucidation molecular pathways governing regulating cellular acquisition required to better understand pathological processes affecting erythropoiesis. Here, we report...
Biotin feeding studies in mice predisposed to developing breast cancer suggest that the tumor load increases by ~100% biotin‐deficient compared with controls, and increase is associated metastasis rather than new tumors. The oncogene c‐myc implicated metastasis, its expression repressed binding of MBP‐1 promoter. Discovery‐based for identifying novel biotin‐containing proteins contains covalently bound biotin, judged mass spectrometry. We hypothesized biotinylation promoter, thereby...
Abstract Tissue iron overload is a frequent pathologic finding in multiple disease states including non-alcoholic fatty liver (NAFLD), neurodegenerative disorders, cardiomyopathy, diabetes, and some forms of cancer. The role iron, as cause or consequence progression observed phenotypic manifestations, remains controversial. In addition, the impact genetic variation on related phenotypes unclear, identification modifiers incomplete. Here, we used Hybrid Mouse Diversity Panel (HMDP),...
Atomically precise nanoclusters (NCs) can serve as an excellent platform for a comprehensive understanding of structure-property relationships. Herein, three structurally similar Cu8 NCs (Cu8-1, Cu8-2 and Cu8-3) have been prepared the photocatalytic phenylacetylene self-coupling reaction. It was found that Cu8-1 NC achieved highest turnover number (TON) 524.4, significantly higher than those most reported catalysts. we propose reasonable two-channel ROS participation mechanism by free...
Abstract Candidalysin is a cytolytic peptide secreted by Candida albicans , an opportunistic human fungal pathogen that was widely spread in clinic and the environment. However, its impacts on development of aquatic organisms remain limited. Herein, we explored developmental effects candidalysin embryonic zebrafish at concentrations from 0.008 to 160 mg/L. In acute exposure, decreased survival rate with EC 50 49.5 mg/L were observed. Meanwhile, high-dose exposure groups (5-160 mg/L),...