A5082623300- Advanced biosensing and bioanalysis techniques
- Nanowire Synthesis and Applications
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- RNA Interference and Gene Delivery
- Muscle metabolism and nutrition
- Mass Spectrometry Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Chemical Reactions and Isotopes
- Exercise and Physiological Responses
- MicroRNA in disease regulation
- Biosimilars and Bioanalytical Methods
- Glycosylation and Glycoproteins Research
- Force Microscopy Techniques and Applications
- Tryptophan and brain disorders
- Monoclonal and Polyclonal Antibodies Research
- S100 Proteins and Annexins
- Microbial Inactivation Methods
- Microfluidic and Bio-sensing Technologies
- RNA and protein synthesis mechanisms
- Ion-surface interactions and analysis
- Genetics, Bioinformatics, and Biomedical Research
- Nanopore and Nanochannel Transport Studies
Institute of Biomedical Chemistry
2016-2025
Center for Genomic Science
2021
Institute of Biomedical Science
2021
The primary objective of analyzing the data obtained in a mass spectrometry-based proteomic experiment is peptide and protein identification, or correct assignment tandem spectrum to one amino acid sequence. Comparison empirical fragment spectra with theoretical predicted matching collected library are commonly accepted strategies proteins identification defining their sequences. Although these approaches widely used appreciably efficient for well-characterized model organisms measured...
Background: Colorectal cancer (CRC) at a current clinical level is still hardly diagnosed, especially with regard to nascent tumors, which are typically asymptotic. Searching for reliable biomarkers of early diagnosis an extremely essential task. Identification specific post-translational modifications (PTM) may also significantly improve net benefits and tailor the process CRC recognition. We examined depleted plasma samples obtained from 41 healthy volunteers 28 patients different stages...
Abstract Despite of multiple systematic studies schizophrenia based on proteomics, metabolomics, and genome-wide significant loci, reconstruction underlying mechanism is still a challenging task. Combination the advanced data for quantitative association study (GWAS) can enhance current fundamental knowledge about molecular pathogenesis schizophrenia. In this study, we utilized proteomic metabolomic assay, high throughput genotyping GWAS study. We identified 20 differently expressed proteins...
Silicon-on-isolator-nanowires (SOI-NWs) were used for the label-free, real-time biospecific detection of hepatitis B marker HBsAg and cancer α-fetoprotein (AFP). Specific protein–protein recognition was carried out using individual NWs that functionalized with antibodies. To solve problem non-specific binding target protein molecules to sensor element use a reference NW immobilized antibodies against non-target proteins proposed. Using SOI-NW surface functionalization allowed fabrication...
Advances in the global personalized medicine market are directly related to innovations and developments molecular genetic technologies. This review focuses on key trends development of these technologies healthcare sector. The existing having an impact evolution reviewed. Efficient measures support proposed.
Herein, we have demonstrated highly sensitive real-time biospecific detection of a protein marker hepatitis C—the core antigen C virus (HCVcoreAg)—using nanowire (NW) biosensor. The primary element the NW-biosensor is chip with p-type conductance, bearing silicon-on-insulator (SOI) structures on its surface. are fabricated by gas-plasma treatment and electron beam lithography. specificity was provided sensitization sensor surface aptamers against HCVcoreAg. influence buffer pH response...
Proteomic and metabolomic research enables quantitation of the molecular profile athletes. Multiomic profiling was conducted using plasma samples collected from 18 male athletes performing aerobic activity (running) at high altitude. Metabolomic detected changes in levels 4-hydroxyproline, methionine, oxaloacetate, tyrosine during recovery period. Furthermore, proteomic revealed expression proteins contributing to function immune system, muscle damage, metabolic fitness performance, as well...
Aptamer-functionalized silicon-on-insulator nanowires were used for the label-free, real-time biospecific detection of cancer marker D-NFATc1 protein in serum.
Kidney diseases pose a serious healthcare problem because of their high prevalence, worsening patients' quality life, and mortality. Patients with kidney are often asymptomatic until disease progression starts. Expensive renal replacement therapy options, such as dialysis or transplant, required for end-stage disease. Early diagnosis pathology is crucial slowing down curbing further damage. This study aimed to analyze the features protein composition blood plasma in patients most common...
ABSTRACT The proteins expressed during the cell cycle determine function and ensure signaling pathway activation in response to environmental influences. Developments structural biology, biophysics, bioinformatics provide information on structure of particular including that changes due post‐translational modification (PTM) amino acid substitutions (AAS), which is essential for understanding protein life cycle. These are PTMs AASs often modulate alter stability localization a cell. PSSKB...
Information about the characteristics of measuring chips according to their storage conditions is great importance for clinical diagnosis. In our present work, we have studied capability detect nanowire biosensors when they are either freshly prepared or been stored one two years in a clean room. Potential DNA oligonucleotides (oDNAs)—synthetic analogues microRNAs (miRNAs) 198 and 429 that associated with development prostate cancer (PCa)—in buffer solution was demonstrated using biosensor...
An approach combining atomic force microscopy ( AFM ) fishing and mass spectrometry MS analysis to detect proteins at ultra‐low concentrations is proposed. Fishing out protein molecules onto a highly oriented pyrolytic graphite surface coated with polytetrafluoroethylene film was carried without application of an external electric field. After that they were visualized by identified . It found injection solution leads charge generation in the solution, potential within measuring cell...
Nanoribbon chips, based on "silicon-on-insulator" structures (SOI-NR chips), have been fabricated. These SOI-NR whose surface was sensitized with covalently immobilized oligonucleotide molecular probes (oDNA probes), employed for the nanoribbon biosensor-based detection of a circular ribonucleic acid (circRNA) marker glioma in humans. The nucleotide sequence oDNA complimentary to target oDNA. latter represents synthetic analogue marker-NFIX RNA. In this way, molecules pure buffer has...
The association between cancer risk and schizophrenia is widely debated. Despite many epidemiological studies, there still no strong evidence regarding the molecular basis for comorbidity these two pathological conditions. vast majority of assays have been performed using clinical records schizophrenic patients or those undergoing treatment monitored sufficient time to find shared features considered We mass spectrometry-based proteomic metabolomic investigations with different phenotypes...
The aim of this study was to determine the influence high-intensity training under extreme conditions (T = 40 °C) on metabolism and immunological reactions athletes. Male triathletes (n 11) with a high level sports performed load testing failure (17 ± 2.7 min) maximum oxygen consumption (64.1 6.4 mL/min/kg). Blood plasma samples were collected before immediately after exercise. Mass spectrometric metabolomic analysis identified 30 metabolites 6 hormones in plasma, which 21 4 changed...
The detection of CA 125 protein in a solution using silicon-on-insulator (SOI)-nanowire biosensor with n-type chip has been experimentally demonstrated. surface nanowires was modified by covalent immobilization antibodies against order to provide the biospecificity target detection. We have demonstrated that signal, which results from biospecific interaction between and covalently immobilized antibodies, increases increase concentration. At that, minimum concentration, at detectable...