A5048985467- Enzyme Structure and Function
- Alzheimer's disease research and treatments
- Photosynthetic Processes and Mechanisms
- Protein Structure and Dynamics
- Proteins in Food Systems
- Protein purification and stability
- Enzyme Production and Characterization
- Photoreceptor and optogenetics research
- Mitochondrial Function and Pathology
- Supramolecular Self-Assembly in Materials
- Enzyme Catalysis and Immobilization
- Prion Diseases and Protein Misfolding
- Food composition and properties
- Shoulder Injury and Treatment
- Peptidase Inhibition and Analysis
- Orthopedic Surgery and Rehabilitation
- Neurological diseases and metabolism
- Acupuncture Treatment Research Studies
- Lipid metabolism and biosynthesis
- Heat shock proteins research
- Metal-Catalyzed Oxygenation Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Hemoglobin structure and function
- Plant Stress Responses and Tolerance
- Soft tissue tumor case studies
Jawaharlal Nehru University
1988-2024
Linköping University
2011-2019
Gandhi Medical College
2018
University of Allahabad
2011
University of Lucknow
2009-2010
TU Dortmund University
2008-2009
University of Crete
1996
Changes in activities of the enzymes involved metabolism active oxygen species were followed homogenates prepared from wheat leaves (Triticum aestivum L.) exposed to strong visible light (600 W m-2). The superoxide dismutase (SOD), ascorbate peroxidase, and monodehydroascorbate reductase increased significantly on prolonged illumination leaves, indicating an increase rate generation species. This was further exacerbated when high stress combined with low temperature (8[deg]C). Our results...
The fluorescence of Nile red (9-diethylamino-5H-benzophenoxazine-5-one) is quenched in aqueous solutions but shows augmented hydrophobic environments. was blue shifted and strongly the presence various amyloid fibrils assayed under acidic as well neutral pH conditions. Fibrils grown from lysozyme insulin (at 1.6 65 °C), transthyretin (TTR) acid unfolded monomer (pH 2.0, 21 °C) or dissociated tetramer starting native protein less conditions 4.4, 37 were detected. also successfully employed...
GRAPES FOR AMYLOIDS: The red wine compound resveratrol can effectively inhibit the formation of IAPP amyloid that is found in type II diabetes. Our vitro inhibition results do not depend on antioxidant activity resveratrol. Further, markedly enhanced cell survival presence also indicates small oligomeric structures are observed during beta-sheet toxic and could be off-pathway assembly products.
Small and effective: The pathological aggregation of amylin (IAPP), which leads to type II diabetes mellitus, is effectively inhibited by small-molecule rhodanine-based inhibitors at nanomolar concentrations. prevention treatment with the inhibitor demonstrated AFM (see image).
Efficient refolding of proteins and prevention their aggregation during folding are vital importance in recombinant protein production finding cures for several diseases. We have used citrate synthase (CS) as a model to understand the mechanism its using known structure-stabilizing cosolvent additives polyol series. Interestingly, no parallel correlation between effect general stabilizing exerted by polyols was observed. Although increasing concentrations increased stability general, yields...
Real-time, residue-specific transition monitoring: IAPP forms amyloid fibrillar deposits in type II diabetes under pathological conditions. The initial events of fibril formation and the from soluble to insoluble, fibrillated can be monitored by kinetic NMR spectroscopy. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted authors. Please note: publisher is...
ABSTRACT Fibrous dysplasia (FD) is a non-malignant fibro-osseous bony lesion in which the involved bone/bones gradually get converted into expanding cystic and fibrous tissue. The underlying defect FD post-natal mutation of GNAS1 gene, leads to proliferation activation undifferentiated mesenchymal cells arresting bone development woven phase ultimately converting them Cherubism hereditary form causative factor transmission autosomal dominant SH3BP2 gene mutation. disease may present two...
Abstract Amyloid fibrils are ordered aggregates that may be formed from disordered, partially unfolded, and fragments of proteins peptides. There several diseases, which due to the formation deposition insoluble β‐sheet protein in various tissue, collectively known as amyloidosis. Here, we have used bovine α‐lactalbumin a model understand mechanism amyloid fibril at pH 1.6 65°C under non‐reducing conditions. is confirmed by Thioflavin T fluorescence atomic force microscopy (AFM). Our finding...
Abstract Polyol co-solvents such as glycerol increase the thermal stability of proteins. This has been explained by preferential hydration favoring more compact native over denatured state. Although polyols are also expected to favor aggregation same mechanism, they have found folding yields some large, aggregation-prone We used homotrimeric phage P22 tailspike protein investigate origin this effect. The is temperature-sensitive and limited monomeric intermediates. At non-permissive...
Klein und zackig: Die pathologische Aggregation von Amylin (IAPP), die bei Diabetes mellitus Typ II auftritt, wird durch nanomolare Konzentrationen an niedermolekularen Inhibitoren auf Rhodaninbasis effektiv gehemmt. Diese Wirkung ließ sich rasterkraftmikroskopisch nachweisen (siehe AFM-Bild).
HdeA and HdeB are dimeric ATP-independent acid-stress chaperones, which protect the periplasmic proteins of enteric bacteria at pH 2.0 4.0, respectively, during their passage through acidic environment mammalian stomach. Despite being structurally similar, they exhibit distinct functional optima conformational prerequisite for chaperone action. undergoes a dimer-to-monomer transition 2.0, whereas remains 4.0. The monomerization exposes its hydrophobic motifs, facilitates interaction with...
α-amylases are industrially important enzymes which used in different starch-based industries. They adapted to environmental conditions like extremes of temperature, pH and salinity. Herein, from Bacillus amyloliquifaciens (BAA) licheniformis (BLA), representing mesophilic thermophilic-like proteins, respectively, have been investigate the effect naturally occurring osmolytes arginine, proline, glycine its methyl derivatives, sarcosine betaine on their refolding. In this study, we shown that...
The theory of Relativity play very-very important Role in Ayurveda, we can say that has a major place Ayurveda and is the only medical system world which all sciences are included, such as chemistry, mathematics, biology, physics etc. Present Ayurveda. science described detail our shastra treatment done on basis elements made UP OF trigunas. There an interconnected Relationship between triguna. We generate May types factors their molecular energy from panchabhutas trigunas it told vedas...
This communication presents a simple method for isolation of oxygen evolving photosystem 2 (PS 2) core complex by solubilisation PS membranes with the nonionic detergent octyl-β-D-thioglucopyranoside (OTG). was free cytochrome (Cyt) f contamination and also lacked 22 10 kDa proteins that may not be directly required primary photochemistry water oxidation. OTG could remove Cyt from 2-core isolated using octyl-β-D-glucopyranoside (OGP). The in complexes potentially interfere spectrophotometric...
Two different kinds of oxygen evolving photosystem II (PSII) core complexes were isolated in the present study by solubilization PSII enriched thylakoid membranes from spinach with non-ionic detergent 6-O-(N-heptylcarbamoyl)-methyl-α-D-glucopyranoside (Hecameg) under conditions. The complex at higher ionic strength was similar to that using octyl-β-D-glucopyranoside (OGP) and lacked 23 17 kDa extrinsic proteins but retained 22 PsbS protein. Solubilization Hecameg lower allowed isolation...
Introduction: Supracondylar fractures of the humerus are very common in pediatric age groups. If displaced, preferred treatment is close reduction with percutaneous K-wire fixation. This study compares functional outcome cross k-wires v/s lateral Gartland type III supracondylar paediatric Materials and Methods: 30 cases gartland children operated closed pinning which 15 were k wires group from January 2015 to June 2017 minimum 4 months follow-up period consideredResults: Functional patients...