Abstract Investigating the interaction patterns at nano-bio interface is a key challenge for safe use of nanoparticles (NPs) to any biological system. The study intends explore role pattern iron oxide nanoparticle (IONP)-bacteria affecting antimicrobial propensity IONP. To this end, IONP with magnetite like atomic arrangement and negative surface potential (n-IONP) was synthesized by co-precipitation method. Positively charged chitosan molecule coating used reverse n-IONP, i.e. positive...

The work investigates the role of interfacial potential in defining antimicrobial propensity ZnO nanoparticle (ZnONP) against different Gram positive and negative bacteria. ZnONPs with surface are tested bacteria varying potentials, ranging -14.7 to -23.6 mV. Chemically synthesized show very high minimum inhibitory concentration 50 100 μg/mL for bacterium, respectively. On other hand, same size but insignificant studied Unlike positively charged nanoparticles, neither Zn(2+) ion nor...

Abstract Nisin inhibits bacterial growth by generating pores in cell membrane and interrupting cell-wall biosynthesis through specific lipid II interaction. However, the role of hinge region C-terminus residues peptide antibacterial action nisin is largely unknown. Here, using molecular dynamics simulations experimental approach, we report that at high concentration regimes nisin, interaction with phospholipids may equally deform membranes even under significantly varying amounts lipid-II....

The present work investigates the role of oxidative stress generated at biosynthesized selenium nanoparticles (SeNPs) interface in defining antimicrobial and anti-biofilm activity. To this end, SeNPs with average size 119 nm were synthesized rapidly during growth

Small and effective: The pathological aggregation of amylin (IAPP), which leads to type II diabetes mellitus, is effectively inhibited by small-molecule rhodanine-based inhibitors at nanomolar concentrations. prevention treatment with the inhibitor demonstrated AFM (see image).

In type 2 diabetics, the hormone amylin misfolds into amyloid plaques implicated in destruction of pancreatic β-cells that make insulin and amylin. The aggregative misfolding is pH-dependent, exposure to acidic basic environments could be physiologically important. Amylin has two ionizable residues between pH 3 9: α-amino group His18. Our approach measuring pKa values for these sites been look at dependence fibrillization variants have only one groups. unstructured N-terminus a near 8.0,...

Due to rapid construction, necessity for raw materials of concrete, especially coarse aggregate, tends increase the danger early exhaustion natural resources. An alternative source would perhaps delay advent this exhaustion. Recycled aggregate (RCA) plays a great role as an material that can replace (NCA) concrete. Previous studies show properties RCA concrete are inferior in quality compared NCA This article attempts study improvement with addition bacteria named Bacillus subtilis. The...

Metal oxide nanoparticles (NPs) have shown enhanced antibacterial effects against many bacteria. Thus, understanding the potential of nickel (NiO NPs) Gram-positive and Gram-negative pathogenic bacteria is an urgent need to enable exploration NiO NP use in biomedical sciences. To this end, NPs were synthesized by microwave assisted hydrothermal synthesis method. The characterized X-ray diffraction (XRD) Fourier Transfer Infrared (FT-IR) UV-visible spectroscopy. morphological features...

We characterized the interaction of amylin with heparin fragments defined length, which model glycosaminoglycan chains associated amyloid deposits found in type 2 diabetes. Binding to positively charged N-terminal half monomeric depends on concentration negatively saccharides but is independent oligosaccharide length. By contrast, fibrillogenesis has a sigmoidal dependence fragment an enhancement observed for oligosaccharides longer than four monomers and leveling off effects beyond 12...

Various types of water-miscible aprotic ionic liquids (ILs) with different cations (1-ethyl-3-methylimidazolium, 1-butyl-3-methylimidazolium, 1-octyl-3-methylimidazolium) and anions (ethylsulfate chloride) were used as co-solvents to investigate the stability lysozyme. Different techniques such fluorescence, thermal absorption, circular dichroism (CD) spectroscopy have been for study. Fluorescence results reveal that addition ILs (1-ethyl-3-methylimidazolium ethyl sulfate...

Total internal reflection fluorescence microscopy has been used to visualize the fibrillization of amylin, a hormone which in aggregated forms plays role type 2 diabetes pathology. Data were obtained at acidic pH where is hindered by charging histidine 18 and slightly basic loss charge on promotes aggregation. The experiments show three types aggregate growth processes. In earliest steps globular seeds are formed with some expanding radially during course reaction. dimensions as well their...

Inside the biological milieu, nanoparticles with photocatalytic activity have potential to trigger cell death non-specifically due production of reactive oxygen species (ROS) upon reacting entities. Silver nanoparticle (AgNP) possessing narrow band gap energy can exhibit high light absorption property and significant activity. This study intends explore effects ROS generated AgNP on antimicrobial cytotoxic propensities. To this end, was synthesized using principle green chemistry from peel...

Parkinson's disease (PD) is characterized by the loss of dopaminergic neurons along with accumulation amyloid plaques alpha-synuclein (αS) as major constituent. αS an intrinsically disordered protein potential to undergo a cascade structural transitions from soluble conformation ordered cross-β-sheet-rich insoluble fibrils. Small molecules like polyphenols and peptides anti-amyloidogenic can mitigate fibrillation in vitro but fail vivo owing poor bioavailability. To overcome this problem,...

Herein the principles of green chemistry were applied to optimize method produce and extract silver nanoparticles during growth bacteria.

Accumulating evidence suggests that amyloid plaque-associated myelin lipid loss as a result of elevated burden might also contribute to Alzheimer's disease. The fibrils are closely associated with lipids under physiological conditions; however, the progression membrane remodeling events leading lipid-fibril assembly remains unknown. Here we first reconstitute interaction Beta 40 (Aβ-40) myelin-like model and show binding Aβ-40 induces extensive tubulation. To look into mechanism tubulation,...