Au–Ag bimetallic nanoclusters with tunable NIR fluorescence have found applications in sensing toxic lead ions <italic>in vitro</italic> and inside cells.

Aggregation of Cu–Zn superoxide dismutase (SOD1) is implicated in the motor neuron disease, amyotrophic lateral sclerosis (ALS). Although more than 140 disease mutations SOD1 are available, their stability or aggregation behaviors membrane environment not correlated with pathophysiology. Here, we use multiple mutational variants to show that absence Zn, and Cu, significantly impacts attachment through two loop regions facilitating driven by lipid-induced conformational changes. These...

Accumulating evidence suggests that amyloid plaque-associated myelin lipid loss as a result of elevated burden might also contribute to Alzheimer's disease. The fibrils are closely associated with lipids under physiological conditions; however, the progression membrane remodeling events leading lipid-fibril assembly remains unknown. Here we first reconstitute interaction Beta 40 (Aβ-40) myelin-like model and show binding Aβ-40 induces extensive tubulation. To look into mechanism tubulation,...

KMP-11 is a small protein that believed to control the overall bilayer pressure of Leishmania parasite. Recent results have suggested membrane binding and presence cholesterol affect efficacy Leishmanial infection, in which plays an important role. Nevertheless, there exists no systematic study interaction with either absence or cholesterol. In this article, we investigated between phospholipid membranes using unsaturated (PC 18:1; 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)) saturated...

Virulent Mycobacterium tuberculosis (MTB) strains cause cell death of macrophages (Mϕ) inside TB granuloma using a mechanism which is not well understood. Many bacterial systems utilize toxins to induce host damage, occurs along with immune evasion. These often use chameleon sequences generate an environment-sensitive conformational switch, facilitating the process infection. The presence yet known for MTB. Here, we show that MTB-secreted immunogenic MPT63 protein undergoes switch from...

Kinetoplastid membrane protein-11 (KMP-11), expressed in all stages of leishmanial life cycle, is considered a potential candidate for leishmaniasis vaccine. KMP-11 found on the surface parasite. Although biological function unknown, we hypothesize from its sequence analysis that it may interact with macrophage and influence entry process parasite into host cell. To validate this hypothesis, have investigated interaction unilamellar anionic phospholipid vesicles explored pore-forming...

Although significant efforts have been devoted to develop nanoparticle-based biopharmaceuticals, it is not understood how protein conformation and nanoparticle surface modulate each other in optimizing the activity and/or toxicity of biological molecules. This particularly important for a protein, which can adopt different conformational states separated by relatively small energy barrier. In this paper, we studied binding-induced switch from β-sheet α-helix MPT63, major secreted...

This study provides a mechanistic description of how the membrane composition and lipid to protein ratio modulate amyloid kinetics yeast prion protein.

Abstract The first step of successful infection by any intracellular pathogen relies on its ability to invade host cell membrane. However, the detailed structural and molecular understanding underlying lipid membrane modification during pathogenic invasion remains unclear. In this study, we show that a specific Leishmania donovani (LD) protein, KMP-11, forms oligomers bridge LD macrophage (MΦ) membranes. This KMP-11 induced interaction between MΦ depends variations in cholesterol (CHOL)...

Kinetoplastid membrane protein-11 (KMP-11) is a small noble immunogenic protein present in the surfaces of all kinetoplastid protozoans. The parasite comprises ergosterol as major component. Although interaction KMP-11 with and role have been implicated process infection survival mechanism parasite, no molecular level understanding available. In this paper, we investigated binding phospholipid model composed 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) absence presence different...

We report here the preparation of an aminoxy amide-based pseudopeptide-derived building block using furanoid sugar molecules. Through cyclo-oligomerization reaction, we generate a hybrid triazole/aminoxy amide macrocycle as-prepared block. The novel conformation has been characterized NMR and molecular modeling studies, which show strong resemblance our synthesized compound to d-,l-α-aminoxy acid-based cyclic peptides that contain uniform backbone chirality. observe can efficiently...

Artificial membrane systems can serve as models to investigate molecular mechanisms of different cellular processes, including transport, pore formation, and viral fusion. However, the current simulacrums such SUVs, GUVs, supported lipid bilayers suffer from issues, namely high curvature, heterogeneity, surface artefacts, respectively. Freestanding membranes provide a facile solution these but developed by various groups use silicon or aluminium oxide wafers for fabrication that involves...

Artificial membrane systems can serve as models to investigate molecular mechanisms of different cellular processes, including transport, pore formation, and viral fusion. However, the current, such SUVs, GUVs, supported lipid bilayers suffer from issues, namely high curvature, heterogeneity, surface artefacts, respectively. Freestanding membranes provide a facile solution these but current developed by various groups use silicon or aluminum oxide wafers for fabrication that involves access...

Abstract Although host membrane is known to play critical roles in the internalization of leishmania parasites inside macrophages (Mϕ), any detailed mechanistic understanding missing. We show here that KMP-11, a small immunogenic protein Leishmania Donovani (LD) facilitates infection process by binding Mϕ through its N-terminal domain (1-19AA). This results phase transition occurs at threshold protein/lipid ratio, which linked change tension. KMP-11 induced also associated with lipid raft...

ABSTRACT Amyloid-beta (Aβ) aggregation mediated neuronal membrane deformation, although poorly understood, is implicated in Alzheimer’s Disease (AD). Particularly, whether Aβ can induce demyelination remains unknown. Here we show that Aβ-40 binds and induces extensive tubulation the myelin vitro. The binding of depends predominantly on lipid packing defect densities electrostatic interactions results rigidification early time scales. Furthermore, elongation into higher oligomeric fibrillar...