A5049719162- Cardiac electrophysiology and arrhythmias
- Cardiac Arrhythmias and Treatments
- Ion channel regulation and function
- ECG Monitoring and Analysis
- Cardiac pacing and defibrillation studies
- Cardiovascular Function and Risk Factors
- Atrial Fibrillation Management and Outcomes
- Neuroscience and Neural Engineering
- Cardiac Ischemia and Reperfusion
- Cardiovascular Effects of Exercise
- Cardiac Imaging and Diagnostics
- Cardiomyopathy and Myosin Studies
- Diabetes Treatment and Management
- Pancreatic function and diabetes
- Fatty Acid Research and Health
- Heart Rate Variability and Autonomic Control
- Electrochemical Analysis and Applications
- Cardiac Arrest and Resuscitation
- Cardiovascular Disease and Adiposity
- Acute Myocardial Infarction Research
- Tissue Engineering and Regenerative Medicine
- Metabolism, Diabetes, and Cancer
- Fuel Cells and Related Materials
- Neuroscience and Neuropharmacology Research
- Heart Failure Treatment and Management
Amsterdam University Medical Centers
2018-2025
University of Amsterdam
2016-2025
Amsterdam Neuroscience
2020-2025
Electrophysiology and Heart Modeling Institute
2015-2024
Amsterdam UMC Location University of Amsterdam
2015-2024
Université de Bordeaux
2015-2024
Inserm
2015-2023
Bordeaux Population Health
2022-2023
Centre de Recherche Cardio-Thoracique de Bordeaux
2015-2022
Military University Nueva Granada
2022
In this report we describe electrophysiologic and histologic findings in hearts endocardially resected preparations from patients with sustained ventricular tachycardias the chronic phase of myocardial infarction. We recorded simultaneously 64 endocardial sites during tachycardia 72 that were operated on for medically intractable tachycardias. Two other underwent heart transplantation, mapping was performed explanted isolated connected to a Langendorff perfusion set-up. During operation 139...
Empagliflozin (EMPA), an inhibitor of the renal sodium-glucose cotransporter (SGLT) 2, reduces risk cardiovascular death in patients with type 2 diabetes. The underlying mechanism this effect is unknown. Elevated cardiac cytoplasmic Na+ ([Na+]c) and Ca2+ ([Ca2+]c) concentrations decreased mitochondrial concentration ([Ca2+]m) are drivers heart failure death. We therefore hypothesised that EMPA would directly modify [Na+]c, [Ca2+]c [Ca2+]m cardiomyocytes.[Na+]c, [Ca2+]c, [Ca 2+]m Na+/H+...
Sodium–glucose cotransporter 2 (SGLT2) inhibitors (SGLT2i) constitute a novel class of glucose-lowering (type 2) kidney-targeted agents. We recently reported that the SGLT2i empagliflozin (EMPA) reduced cardiac cytosolic Na+ ([Na+]c) and Ca2+ ([Ca2+]c) concentrations through inhibition Na+/H+ exchanger (NHE). Here, we examine (1) whether dapagliflozin (DAPA) canagliflozin (CANA) also inhibit NHE reduce [Na+]c; (2) structural model for interaction to NHE; (3) what extent affect haemodynamic...
The mechanism of ECG changes and arrhythmogenesis in Brugada syndrome (BS) patients is unknown.A BS patient without clinically detected cardiac structural abnormalities underwent transplantation for intolerable numbers implantable cardioverter/defibrillator discharges. patient's explanted heart was studied electrophysiologically histopathologically. Whole-cell currents were measured HEK293 cells expressing wild-type or mutated sodium channels from the patient. right ventricular outflow tract...
Background — Progressive activation delay starting at long coupling intervals of premature stimuli has been shown to correlate with sudden cardiac death in patients hypertrophic cardiomyopathy. The purpose this study was elucidate the mechanism increased chronically diseased myocardium. Methods and Results High-resolution unipolar mapping (105, 208, or 247 recording sites interelectrode distances 0.8, 0.5, 0.3 mm, respectively) epicardial electrical activity carried out during stimulation 11...
Conduction velocities along longitudinal (vL) and transverse (vT) fiber axes were determined in isolated porcine hearts from subepicardial activation patterns that produced by local stimulation measured with a multiterminal electrode. In some of the experiments extracellular [K+] ([K+]o) transmembrane potentials recorded. During normal perfusion vL vT (cm/sec) 50.08 +/- 2.13, (SE) 21.08 0.97. After 3 to 5 min global ischemia, decreased approximately 30 13 cm/sec. Before occurrence total...
An experimental approach is described to quantitate inhomogeneity in extracellular K concentration ([K+]out) the presence of ischemia and relate this electrophysiologic changes. Extracellular potassium local direct-current electrograms from same sites were measured isolated perfused pig hearts with use multiple electrodes. Dispersion [K+]out under three conditions: (1) during regional "central zone" "borderzone", (2) global ischemia, (3) perfusion heart a high-K perfusate. Inhomogeneity was...
The aim of the study was to assess occurrence triggered activity and automaticity in ventricular trabeculae from failing human hearts normal rabbit during exposure a altered extracellular environment.Ventricular were harvested (from patients undergoing cardiac transplantation) (combined volume pressure overload). Trabeculae superfused with Tyrode solution followed by modified solution, which mimicked milieu severe heart failure. Modified contained low potassium (3.0 mM), magnesium (0.4...
In 18 dogs on total cardiopulmonary bypass, the average interval between local activations during artificially induced ventricular fibrillation (VF interval) was measured from extracellular electrograms, simultaneously recorded up to 32 sites. VF intervals were used as an index of refractoriness, based assumption that fibrillation, cells are reexcited soon they have recovered their excitability. support this, microelectrode recordings in two hearts did not show a diastolic successive action...
Animal studies revealed that the hyperpolarization-activated pacemaker current, If, contributes to action potential (AP) generation in sinoatrial node (SAN) and significantly determines heart rate. If is becoming a novel therapy target modulate Yet, no have demonstrated functionally present pacemaking human SAN. We aimed study properties In patient undergoing SAN excision, we identified using epicardial activation mapping. From here, isolated myocytes recorded APs patch-clamp techniques....
Background— Atrial fibrosis is an important component of the arrhythmogenic substrate in patients with atrial fibrillation (AF). We studied effect interstitial on conduction velocity (CV) left appendage AF. Methods and Results— Thirty-five appendages were obtained during AF surgery. Preparations superfused stimulated at 100 beats per minute. Activation was recorded optical mapping. Longitudinal CV (CV L ), transverse T activation times (>2 mm distance) measured. Interstitial collagen...
We conducted a multicenter study to evaluate mapping and ablation of ventricular fibrillation (VF) substrates or VF triggers in early repolarization syndromes (ERS) J-wave syndrome (JWS).We studied 52 patients with ERS (4 women; median age, 35 years) recurrent episodes. Body surface electrocardiographic imaging endocardial epicardial electroanatomical both ventricles were performed during sinus rhythm for localization triggers, substrates, drivers. Ablations on defined as areas that had late...