A5028538121- Neuroscience and Neuropharmacology Research
- Nerve injury and regeneration
- Ion channel regulation and function
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Nicotinic Acetylcholine Receptors Study
- Neurogenesis and neuroplasticity mechanisms
- Advanced biosensing and bioanalysis techniques
- Mitochondrial Function and Pathology
- Advanced Biosensing Techniques and Applications
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- Alzheimer's disease research and treatments
- Neuropeptides and Animal Physiology
- Advanced Fluorescence Microscopy Techniques
- Biochemical and Structural Characterization
- Receptor Mechanisms and Signaling
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Nanofabrication and Lithography Techniques
- Sleep and Wakefulness Research
- RNA Interference and Gene Delivery
- Microtubule and mitosis dynamics
- Retinal Development and Disorders
National Institute of Advanced Industrial Science and Technology
2002-2024
NTL Institute for Applied Behavioral Science
2020
Midorigaoka Hospital
1997-2019
Neuroscience Institute
2009
Osaka University
1993-1996
Abstract Background Proneurotrophins and mature neurotrophins elicit opposite effects via the p75 neurotrophin receptor (p75 NTR ) Trk tyrosine kinase receptors, respectively; however molecular roles of proneurotrophins in CNS are not fully understood. Results Based on two rare single nucleotide polymorphisms (SNPs) human brain-derived neurotrophic factor (BDNF) gene, we generated R125M-, R127L- R125M/R127L-BDNF, which have amino acid substitution(s) near cleavage site between pro-...
Brain-derived neurotrophic factor (BDNF) exerts multiple biological functions in the CNS. Although BDNF can control transcription and protein synthesis, it still remains open to question whether regulates lipid biosynthesis. Here we show that elicits cholesterol biosynthesis cultured cortical hippocampal neurons. Importantly, elicited synthesis neurons, but not glial cells. Quantitative reverse transcriptase-PCR revealed stimulated of enzymes biosynthetic pathway. BDNF-induced increases were...
Abstract Neuronal differentiation—the development of neurons from neural stem cells—involves neurite outgrowth and is a key process during the regeneration functions. In addition to various chemical signaling mechanisms, it has been suggested that thermal stimuli induce neuronal differentiation. However, function physiological subcellular thermogenesis differentiation remains unknown. Here we create methods manipulate observe local intracellular temperature, investigate effects noninvasive...
On the substrate carrying a sub-wavelength grating covered with thin metal layer, fluorescent dye-labeled cell was observed by fluorescence microscope. The intensity more than 20 times greater that on an optically flat glass substrate. Such great enhancement from labeled cells bound to due excitation coupled surface plasmon resonance. application of two-dimensional detection and microscopy appears offer promising method taking highly sensitive images.
Micropatterned composite membranes of polymerized and fluid lipid bilayers were constructed on solid substrates. Lithographic photopolymerization a diacetylene-containing phospholipid, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC), subsequent removal nonreacted monomers by detergent solution (0.1 M sodium dodecyl sulfate (SDS)) yielded patterned polymeric bilayer matrix the substrate. Fluid phosphatidylcholine from egg yolk (egg-PC) incorporated into lipid-free wells...
Neuronal activity regulates the synaptic strength of neuronal networks. However, it is still unclear how diminished changes connection patterns in circuits. To address this issue, we analyzed connectivity and relevant mechanisms using hippocampal cultures which developmental synaptogenesis had occurred. We show that diminution network mature circuit promotes reorganization circuits via NR2B subunit-containing NMDA-type glutamate receptors (NR2B-NMDARs), mediate silent synapse formation....
High-resolution volume imaging techniques, such as lattice light-sheet microscopy (LLSM), generate vast and complex datasets that demand advanced analytical approaches to uncover biologically meaningful insights. While LLSM's high spatial temporal resolution provides critical data for understanding cellular processes, distinguishing subtle differences between cells in distinct states remains challenging. Here, using an adaptive, human-interpretable kernel-based calculation strategy, we...
We examined the possibility that basic fibroblast growth factor (bFGF) is involved in synaptic transmissions. found bFGF rapidly induced release of glutamate and an increase intracellular Ca<sup>2+</sup> concentration through voltage-dependent channels cultured cerebral cortical neurons. also evoked a significant influx Na<sup>+</sup>. Tetanustoxin inhibited bFGF-induced release, revealing triggered exocytosis. The mitogen-activated protein kinase (MAPK) pathway was required for these acute...
Abstract Oligomers of intracellular amyloid β protein (Aβ) are strongly cytotoxic and play crucial roles in synaptic transmission cognitive function Alzheimer’s disease (AD). However, there is currently no AD model mouse which to specifically analyze the Aβ oligomers only. We have now developed a novel mouse, an Aβ-GFP transgenic (Aβ-GFP Tg), that expresses GFP-fused human 1-42 protein, forms only within neurons throughout their life. The fusion proteins expressed mainly hippocampal CA1-CA2...
Dysferlin is a sarcolemmal protein that defective in Miyoshi myopathy and limb-girdle muscular dystrophy type 2B, involved repair. Primary cultured myoblasts myotubes established from patient muscle biopsies have been widely utilized to explore the molecular mechanism of dysferlinopathy.The purpose this study was possible utility dermal fibroblasts dysferlin-deficient patients SJL mice as tool for studying dysferlinopathy.Dysferlin expression analyzed by immunoblotting immunocytochemistry....
Exogenous gene expression is a fundamental and indispensable technique for testing function in neurons. Several ways to express exogenous genes neurons are available, but each method has pros cons. The lentivirus vector useful high efficiency transfer stabilizes via genome integration, this integration may destroy the host genome. Epstein-Barr virus (EBV)-derived (EB vector) an accessible human cell lines because not integrated into stays nucleus as episome. However, there been no report on...
Abstract A plastic dish with a wavelength-scale periodic structure at bottom panel was integrally molded and coated thin metal films. The called plasmonic applied to bioimaging under fluorescence microscope. On the substrate, enhanced electric field based on grating-coupled surface plasmon resonance (GC-SPR) can provide an fluorescence. In this study, two kinds of cells, human embryonic kidney (HEK) cells neuronal were observed in our dish. Fluorescence images HEK above 10 times brighter...
Abstract N ‐methyl‐ d ‐aspartate receptors (NMDARs) are known to play critical roles in the development of nervous system, and their expression is regulated an activity‐dependent fashion during development. However, regulation NMDAR after circuit formation less well understood. To examine this, we performed patch‐clamp recordings from chick cerebral neurons activity‐controlled culture. Analysis channels before synapse showed that there two components channel open kinetics. The major slow...
Abstract We report a protein factor(s) contained in the conditioned medium (CM) of Mg 2+ ‐free treatment induced synaptic potentiation. This type potentiation shared different pathway from those by neurotrophins. Neurotrophins were confirmed to induce dissociated chick neurons. Furthermore, K252a, an inhibitor tyrosine kinase, abolished this Nevertheless, CM was not blocked K252a. In addition, prepared neurons similar rat and mouse These results suggest that factor is novel molecule for...
Effects of transduction activity and transmission on synaptogenesis chick cerebral neurones in dissociated cell culture were studied electrophysiologically using two blockers for these activities, tetrodotoxin (TTX) 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), respectively. CNQX inhibited the increase evoked EPSCs more effectively than TTX, whereas both similarly reduced miniature (Minis). These data indicated that not only transduction-dependent but also transduction-independent spontaneous...
Abstract Transmitter release at an excitatory synapse has two components, fast synchronous and slow asynchronous transmitter release. Using the whole cell recording technique, we investigated developmental properties of neurotransmitter release, which is composed components in intermediate medial part hyperstriatum ventral (IMHV) chicks during critical period for imprinting. Analysis paired‐pulse responses revealed that depression postsynaptic currents (EPSCs), driven mainly by was...
Incubation of a human fibrosarcoma cell line HT-1080 in increasing concentration Ca2+ was found to enhance endocytic internalization fluid phase marker, horseradish peroxidase. At 16.8 mM Ca2+, generation the effect required incubation for more than 45 min. The reversed by removal excess ion 30 Monitoring intracellular showed that induced transient large influx followed recovery 230 +/- 50 nM instead normal level 83 5 nM. activation not inhibited inhibitors protein kinases nor cAMP...