A5075384535- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Autism Spectrum Disorder Research
- Neuroscience and Neuropharmacology Research
- RNA Interference and Gene Delivery
- Electromagnetic Fields and Biological Effects
- Signaling Pathways in Disease
- Lipid Membrane Structure and Behavior
- Streptococcal Infections and Treatments
- Neuropeptides and Animal Physiology
- Bacteriophages and microbial interactions
- Neonatal and Maternal Infections
- Viral Infectious Diseases and Gene Expression in Insects
- Axon Guidance and Neuronal Signaling
- Endoplasmic Reticulum Stress and Disease
- RNA regulation and disease
- Biochemical effects in animals
- Parkinson's Disease Mechanisms and Treatments
- Advanced biosensing and bioanalysis techniques
- Redox biology and oxidative stress
- Nuclear Receptors and Signaling
- Microtubule and mitosis dynamics
- Toxin Mechanisms and Immunotoxins
- Alcoholism and Thiamine Deficiency
- Regulation of Appetite and Obesity
Kanazawa Institute of Technology
2021-2024
Japan Science and Technology Agency
2012-2024
National Institute of Advanced Industrial Science and Technology
2012-2023
Osaka University
1992-2020
NTL Institute for Applied Behavioral Science
2020
Kanazawa University
2020
Biomedical Research Institute
2017
Kitasato University
2009
Midorigaoka Hospital
2002-2003
National Institutes of Health
2003
Although brain-derived neurotrophic factor (BDNF) regulates numerous and complex biological processes including memory retention, its extremely low levels in the mature central nervous system have greatly complicated attempts to reliably localize it. Using rigorous specificity controls, we found that antibodies reacting either with BDNF or pro-peptide both stained large dense core vesicles excitatory presynaptic terminals of adult mouse hippocampus. Both moieties were ∼10-fold more abundant...
Brain-derived neurotrophic factor (BDNF) plays an important role in synaptic plasticity but the underlying signaling mechanisms remain unknown. Here, we show that BDNF rapidly recruits full-length TrkB (TrkB-FL) receptor into cholesterol-rich lipid rafts from nonraft regions of neuronal plasma membranes. Translocation TrkB-FL was blocked by Trk inhibitors, suggesting a tyrosine kinase translocation. Disruption depleting cholesterol cell surface ligand-induced Moreover, disruption prevented...
Abstract Background Proneurotrophins and mature neurotrophins elicit opposite effects via the p75 neurotrophin receptor (p75 NTR ) Trk tyrosine kinase receptors, respectively; however molecular roles of proneurotrophins in CNS are not fully understood. Results Based on two rare single nucleotide polymorphisms (SNPs) human brain-derived neurotrophic factor (BDNF) gene, we generated R125M-, R127L- R125M/R127L-BDNF, which have amino acid substitution(s) near cleavage site between pro-...
Brain-derived neurotrophic factor (BDNF) exerts multiple biological functions in the CNS. Although BDNF can control transcription and protein synthesis, it still remains open to question whether regulates lipid biosynthesis. Here we show that elicits cholesterol biosynthesis cultured cortical hippocampal neurons. Importantly, elicited synthesis neurons, but not glial cells. Quantitative reverse transcriptase-PCR revealed stimulated of enzymes biosynthetic pathway. BDNF-induced increases were...
Most growth factors are initially synthesized as precursor proteins and subsequently processed into their mature form by proteolytic cleavage, resulting in simultaneous removal of a pro-peptide. However, compared with that form, the biological role pro-peptide is poorly understood. Here, we investigated brain-derived neurotrophic factor (BDNF) first showed expressed secreted hippocampal tissues cultures, respectively. Interestingly, found BDNF directly facilitates long-term depression (LTD),...
Brain-derived neurotrophic factor (BDNF) polymorphism is associated with the pathophysiology of several neurodegenerative disorders, including Huntington's disease. In view these data and involvement huntingtin in intracellular trafficking, we examined transport release Val66Val BDNF (Val-BDNF) Val66Met (Met-BDNF) transfected striatal knock-in cells expressing wild-type or mutant full-length huntingtin. Colocalization studies specific markers for endoplasmic reticulum showed no differences...
Brain-derived neurotrophic factor (BDNF) importantly regulates learning and memory supports the survival of injured neurons. Reduced BDNF levels have been detected in brains Alzheimer's disease (AD) patients but exact role pathophysiology disorder remains obscure. We recently shown that reduced signaling receptor TrkB aggravates impairment APPswe/PS1dE9 (APdE9) mice, a model AD. The present study examined influence Bdnf gene deficiency (heterozygous knockout) on spatial learning, spontaneous...
Brain-derived neurotrophic factor (BDNF) is a growth that promotes the survival and of developing neurons. It also enhances circuit formation to synaptic transmission for mature neurons in brain. However, reduced BDNF expression single nucleotide polymorphisms (SNP) are reported be associated with functional deficit disease development brain, suggesting crucial molecule brain health. Interestingly, expressed hypothalamus appetite energy metabolism. Previous reports demonstrated knockout mice...
Abstract Our open-label pilot study showed that supplementation with docosahexaenoic acid (DHA) increased serum brain-derived neurotrophic factor (BDNF) levels and there might be an association between changes in BDNF reduced psychological distress. Animal research has indicated a DHA-enriched diet increases the brain. In this randomized double-blind controlled trial of severely injured patients vulnerable to posttraumatic stress disorder (PTSD) depression, we examined whether DHA are...
Most growth factors are initially synthesized as precursors then cleaved into bioactive mature domains and pro-domains, but the biological roles of pro-domains poorly understood. In present study, we investigated pro-domain (or pro-peptide) brain-derived neurotrophic factor (BDNF), which promotes neuronal survival, differentiation synaptic plasticity. The BDNF pro-peptide is a post-processing product precursor BDNF. Using surface plasmon resonance biochemical experiments, first demonstrated...
Abstract We reported previously that BDNF induced glutamate release was dependent on intracellular Ca 2+ but not extracellular in cerebellar neurons (Numakawa et al., 1999 ). It revealed the through a non‐exocytotic pathway (Takei 1998 ; Numakawa In present study, we monitored dynamics of and Na + neurons, investigated possibility reverse transport mediated by BDNF. As reported, increased level. found increase completely blocked xestospongin C, an IP 3 receptor antagonist, U‐73122, PLC‐γ...
Thioredoxin-1 (TRX) plays important roles in cellular signaling by controlling the redox state of cysteine residues target proteins. TRX is released response to oxidative stress and shows various biologic functions from extracellular environment. However, mechanism which transduces signal into cells remains unclear. Here we report that modification at active site promotes internalization cells. TRX-C35S, residue 35 was replaced with serine, internalized more effectively than wild-type human...
Abstract Chronic stress is associated with anxiety and depressive disorders can cause weight gain. Ca 2+ -dependent activator protein for secretion 2 (CAPS2) involved in insulin release. Caps2 knockout (KO) mice exhibit decreased body weight, reduced glucose-induced release abnormal psychiatric behaviors. We chronically administered the hormone corticosterone (CORT), which induces anxiety/depressive-like behavior normally increases plasma levels, via drinking water 10 weeks we examined...