A5085951307- Redox biology and oxidative stress
- Neonatal Health and Biochemistry
- Glutathione Transferases and Polymorphisms
- Genomics, phytochemicals, and oxidative stress
- Helicobacter pylori-related gastroenterology studies
- Heme Oxygenase-1 and Carbon Monoxide
- Renal Diseases and Glomerulopathies
- Heat shock proteins research
- Sulfur Compounds in Biology
- Endoplasmic Reticulum Stress and Disease
- Inflammatory mediators and NSAID effects
- Metabolism and Genetic Disorders
- Muscle Physiology and Disorders
- Neonatal Respiratory Health Research
- Biochemical effects in animals
- Gastric Cancer Management and Outcomes
- T-cell and Retrovirus Studies
- Methemoglobinemia and Tumor Lysis Syndrome
- Liver Disease Diagnosis and Treatment
- Selenium in Biological Systems
- Neonatal and fetal brain pathology
- Vitamin C and Antioxidants Research
- Cardiac Imaging and Diagnostics
- Retinal Development and Disorders
- Retinal Diseases and Treatments
Kobe University
2001-2025
Osaka University
1997-2025
Sapporo Medical University
2000-2024
Japan Anti Tuberculosis Association
2022-2024
Nagasaki University
2024
Otaru Municipal Hospital
2022-2023
Osaka Ekisaikai Hospital
2023
Otsu Red Cross Hospital
2006-2023
High Energy Accelerator Research Organization
2022
Shizuoka Saiseikai General Hospital
2004-2021
Recent works have shown the importance of reduction/oxidation (redox) regulation in various biological phenomena. Thioredoxin (TRX) is one major components thiol reducing system and plays multiple roles cellular processes such as proliferation, apoptosis, gene expression. To investigate molecular mechanism TRX action, we used a yeast two-hybrid to identify TRX-binding proteins. One candidates, designated thioredoxin-binding protein-2 (TBP-2), was identical vitamin D3 up-regulated protein 1...
Oxidative stresses such as UV irradiation to mammalian cells triggers a variety of oxistress responses including activation transcription factors. Recently, nuclear factor-κB (NF-κB) has been shown be under oxidoreduction (redox) regulation controlled by thioredoxin (TRX), which is one major endogenous redox-regulating molecules with thiol reducing activity. In order elucidate where in the cellular compartment TRX participates NF-κB regulation, we investigated intracellular localization TRX....
Abstract An activated AKT pathway underlies the pathogenesis of soft tissue sarcoma (STS), with over-expressed phosphorylated (p-AKT) correlating a poor prognosis in subset STS cases. Recently, eribulin, microtubule dynamics inhibitor, has demonstrated efficacy and is approved patients advanced/metastatic liposarcoma breast cancer. However, mechanisms eribulin resistance and/or insensitivity remain largely unknown. In this study, we that an increased p-AKT level was associated cells. We...
Thioredoxin (Trx) is a ubiquitous intracellular protein disulfide oxidoreductase with CXXC active site that can be released by various cell types upon activation. We show here Trx chemotactic for monocytes, polymorphonuclear leukocytes, and T lymphocytes, both in vitro the standard micro Boyden chamber migration assay vivo mouse air pouch model. The potency of action all leukocyte populations nanomolar range, comparable known chemokines. However, does not increase Ca2+ its activity inhibited...
Glutathione peroxidase catalyzes the reduction of hydrogen peroxide and organic hydroperoxide by glutathione functions in protection cells against oxidative damage. exists several forms that differ their primary structure localization. We have also shown selenoprotein P exhibits a peroxidase-like activity (Saito, Y., Hayashi, T., Tanaka, A., Watanabe, Suzuki, M., Saito, E., Takahashi, K. (1999) J. Biol. Chem. 274, 2866–2871). To understand physiological significance diversity among these...
Glutaredoxin (GRX) is a small dithiol protein involved in various cellular functions, including the redox regulation of certain enzyme activities. GRX functions via disulfide exchange reaction by utilizing active site Cys-Pro-Tyr-Cys. Here we demonstrated that overexpression protected cells from hydrogen peroxide (H2O2)-induced apoptosis regulating state Akt. Akt was transiently phosphorylated, dephosphorylated, and then degraded cardiac H9c2 undergoing H2O2-induced apoptosis. Under stress,...
Transgenic (Tg) mice overexpressing human thioredoxin (TRX), a small redox-active protein, were produced to investigate the role of protein in variety stresses. Bone marrow cells from TRX-Tg more resistant ultraviolet C-induced cytocide compared with those wild type (WT) C57BL/6 mice. exhibited extended median and maximum life spans WT Telomerase activity spleen tissues was higher than that These results suggest overexpression TRX resistance against oxidative stress possible extension span...
Background Glutathione (GSH) deficiency is common in HIV‐infected individuals and associated with impaired T cell function survival. N‐acetylcysteine (NAC) used to replenish GSH that has been depleted by acetaminophen overdose. Studies here test oral administration of NAC for safe effective replenishment HIV infection. Design Oral a randomized, 8‐week double‐blind, placebo‐controlled trial followed optional open‐label drug up 24 weeks. Subjects HIV‐infected, low GSH, CD4 cells < 500 μL −1...
Thioredoxin (Trx), a redox enzyme with conserved active site (Cys-32–Gly–Pro–Cys-35), is induced and secreted into circulation in response to inflammation. Studies here demonstrate that elevating Trx levels either by i.v. injection of recombinant or stimulating release Trx-transgenic mice dramatically blocks lipopolysaccharide (LPS)-stimulated neutrophil migration the murine air pouch chemotaxis model. Furthermore, we show leukocyte recruitment chemokines KC/GROα, RANTES (regulated upon...
Objective. The purpose of this study was to determine whether end-tidal carbon monoxide (CO) corrected for ambient CO (ETCOc), as a single measurement or in combination with serum total bilirubin (STB) measurements, can predict the development hyperbilirubinemia during first 7 days life. Methods. From 9 multinational clinical sites, 1370 neonates completed cohort from February 20, 1998, through 22, 1999. Measurements both ETCOc and STB were performed at 30 ± 6 hours life; also measured 96 12...
In the light of accumulating evidence for occurrence spontaneous cortical spreading depression and peri-infarct depolarizations in human brain injured by trauma or aneurysmal subarachnoid haemorrhage, we used DC electrode recording laser speckle imaging to study relationship between depolarization events perfusion ischaemic, gyrencephalic brain. 14 adult male cats anaesthetized with chloralose, one cerebral hemisphere was exposed middle artery occluded. Surface core penumbral territories...
We report here the second evidence of retrotransposition L1, which was found inserted into dystrophin gene a patient, causing Duchenne muscular dystrophy (DMD).When PCR used to amplify region en- compassing exon 44 from genomic DNA two Japanese brothers with DMD, it be -600 bp larger than expected.Both normal and abnormally large products were amplified their mother.However, maternal grandparents did not have abnormal allele, mutation must therefore occurred in mother.Anal- ysis nucleotide...
Background— Adriamycin (ADR) is an anticancer drug known to cause severe cardiac toxicity by generating free radicals. We investigated the role of a redox-regulating molecule, thioredoxin-1 (TRX1), in ADR-induced cardiotoxicity. Methods and Results— The vitro study showed that TRX1 was dose-dependently increased concomitant with formation hydroxyl radicals ADR-treated neonatal rat cardiomyocytes. Lactate dehydrogenase–releasing assay treatment recombinant human suppressed cardiomyocyte...
Abstract Purpose: Thioredoxin overexpression is suggested to be associated with resistance several chemotherapeutic agents in vitro. In the present study, it has been studied whether or not high thioredoxin expression docetaxel therapy breast cancer patients. Patients and Methods: Sixty-three primary patients were treated (60 mg/m2, q3w) for four cycles neoadjuvant setting. Expression of thioredoxin, estrogen receptor (ER), p53, BRCA-1, Bcl-2 tumor tissues obtained before was by...
To assess early bilirubin toxicity, a study was made of auditory brainstem responses in relation to total levels as well unbound 56 hyperbilirubinemic infants (total greater than or equal 15.0 mg/dL) and 24 who did not have jaundice. The latencies wave I at 85 dB HL (hearing level) were significantly those the control group. V with 1.0 microgram/dL (group C) group less 0.5 A) B). There no significant differences I-V interpeak latency between infants. Thirty 80 showed prolonged peak (greater...