A5041739774- Bacterial Genetics and Biotechnology
- Dermatology and Skin Diseases
- Biochemical and Structural Characterization
- Cancer Research and Treatments
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Protist diversity and phylogeny
- Monoclonal and Polyclonal Antibodies Research
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Psoriasis: Treatment and Pathogenesis
- Nail Diseases and Treatments
- Melanoma and MAPK Pathways
- Bacterial biofilms and quorum sensing
- Muscle Physiology and Disorders
- Mesenchymal stem cell research
- Antifungal resistance and susceptibility
- CAR-T cell therapy research
- IL-33, ST2, and ILC Pathways
- Gene Regulatory Network Analysis
- Transgenic Plants and Applications
- Toxin Mechanisms and Immunotoxins
- Anorectal Disease Treatments and Outcomes
- Breast Implant and Reconstruction
- Evolution and Genetic Dynamics
Southern Medical University
2025
Massachusetts Institute of Technology
2009-2025
Broad Institute
2023-2025
Massachusetts General Hospital
2013-2025
Yunnan Academy of Agricultural Sciences
2025
Stanford University
2018-2024
Bioengineering Center
2024
San Francisco VA Health Care System
2021-2023
San Francisco VA Medical Center
2019-2020
University of California, San Francisco
2018
Commensals rule the MAITrix Mucosal-associated invariant T (MAIT) cells play an important role in mucosal homeostasis. MAIT recognize microbial small molecules presented by major histocompatibility complex class Ib molecule MR1. are absent germ-free mice, and mechanisms which microbiota control cell development unknown (see Perspective Oh Unutmaz). Legoux et al. show that, of within thymus is governed bacterial product 5-(2-oxopropylideneamino)-6- d -ribitylaminouracil, rapidly traffics from...
How defined microbes influence the skin immune system remains poorly understood. Here we demonstrate that Corynebacteria, dominant members of microbiota, promote a dramatic increase in number and activation subset γδ T cells. This effect is long-lasting, occurs independently other microbes, is, part, mediated by interleukin (IL)-23. Under steady-state conditions, impact Corynebacterium discrete noninflammatory. However, when applied to host fed high-fat diet, accolens alone promotes...
Certain bacterial colonists induce a highly specific T cell response. A hallmark of this encounter is that adaptive immunity develops preemptively, in the absence an infection. However, functional properties colonist-induced cells are not well defined, limiting our ability to understand anticommensal and harness it therapeutically. We addressed both challenges by engineering skin bacterium Staphylococcus epidermidis express tumor antigens anchored secreted or cell-surface proteins. Upon...
Spatial asymmetry is crucial to development. One mechanism for generating involves the localized synthesis of a key regulatory protein that diffuses away from its source, forming spatial gradient. Although gradients are prevalent in eukaryotes, at both tissue and intracellular levels, it unclear whether freely diffusible proteins can form within bacterial cells given their small size speed diffusion. Here, we show bacterium Caulobacter crescentus generates gradient active, phosphorylated...
Pluripotent bone marrow–derived side population (BM-SP) stem cells have been shown to repopulate the hematopoietic system and contribute skeletal cardiac muscle regeneration after transplantation. We tested BM-SP for their ability regenerate heart using a model of cardiomyopathy muscular dystrophy that lacks δ-sarcoglycan. The absence δ-sarcoglycan produces microinfarcts in should recruit regenerative cells. Additionally, sarcoglycan expression transplantation mark successful cell maturation...
ABSTRACT In Caulobacter crescentus , progression through the cell cycle is governed by periodic activation and inactivation of master regulator CtrA. Two phosphorelays, each initiating with histidine kinase CckA, promote CtrA driving its phosphorylation inactivating proteolysis. Here, we examined whether CckA phosphorelays also influence downregulation We demonstrate that bifunctional, capable acting as either a or phosphatase to drive inactivation, respectively, By identifying mutations...
Pluripotent bone marrow–derived side population (BM-SP) stem cells have been shown to repopulate the hematopoietic system and contribute skeletal cardiac muscle regeneration after transplantation. We tested BM-SP for their ability regenerate heart using a model of cardiomyopathy muscular dystrophy that lacks δ-sarcoglycan. The absence δ-sarcoglycan produces microinfarcts in should recruit regenerative cells. Additionally, sarcoglycan expression transplantation mark successful cell maturation...
Background: Hidradenitis suppurativa is a chronic, debilitating disease that difficult to treat. Once medical management fails, wide local excision offers the best chance for cure. However, resultant wound often proves too large or contaminated immediate closure. Methods: The authors performed retrospective chart review of hidradenitis cases managed surgically between 2005 and 2010. Data collected included patient characteristics, method, outcomes. Approximately half patients received...
For patients with advanced melanoma, primary and secondary resistance to selective BRAF inhibition remains one of the most critically compelling challenges. One rationale argues that novel biologically informed strategies are needed maximally cripple melanoma cells up front before compensatory mechanisms emerge. As p53 is uncommonly mutated in restoration its function represents an attractive adjunct inhibition.Thirty-seven BRAF(V600E)-mutated lines were subjected synergy studies vitro using...
Yunnan is located on the southwest border of China, with a complex geographical environment and rich biodiversity, which first stop for many migratory pests to enter China. In recent years, Ceracris kiangsu has migrated into China through China-Laos line. The C. shown typical characteristics locusts, seriously jeopardized ecological security, biosecurity food security order prevent control from source as soon possible, this study used hotspot analysis trajectory clarify migration dynamics,...
The bacterial genus Staphylococcus comprises diverse species that colonize the skin as commensals but can also cause infection. Previous work identified a family of serine hydrolases termed fluorophoshonate-binding (Fphs) in pathogenic bacteria aureus, one which, FphB, functions virulence factor. Using combination bioinformatics and activity-based protein profiling (ABPP), we identify homologues these enzymes related commensal epidermidis. Two S. aureus Fph were not ABPP, several candidate...
ABSTRACT The ubiquitous skin colonist Staphylococcus epidermidis elicits a CD8 + T cell response pre-emptively, in the absence of an infection 1 . However, scope and purpose this anti-commensal immune program are not well defined, limiting our ability to harness it therapeutically. Here, we show that also induces potent, durable, specific antibody is conserved humans non-human primates. A series S. cell-wall mutants revealed surface protein Aap predominant target. By colonizing mice with...
Abstract Early-life microbial colonization has been shown to impart long-lasting effects on host fitness. Because mucosal-associated invariant T (MAIT) cells are predominantly located in tissues colonized by the microbiota and characterized their recognition of microbial-derived riboflavin intermediates, they thought be particularly dependent microbiota. However, little is known about development how contribute tissue physiology. MAIT accumulated barrier between 2 3 weeks age, suggesting...
SUMMARY Commensal skin bacteria elicit potent, antigen-specific immune responses in the without barrier breach or visible inflammation. While microbial modulation of homeostasis has profound consequences for epithelial health and inflammatory diseases, mechanisms microbe-immune crosstalk are largely unknown. A key to mechanistic work been genetic intractability one most prevalent colonists, Staphylococcus epidermidis ( S. ). Here, we develop a novel method create library mutants with defined...
Sir:FigureIn the field of breast reconstruction, many surgical methods exist to minimize complications while maximizing aesthetic result.1–3 However, all techniques still rely on subjective judgment assess symmetry, often most concerning parameter for patient. This assessment is challenging and increases operative time through patient repositioning and, often, guesswork. In this study, we demonstrate a novel laser projection grid that assists in evaluating symmetry during two cases...