A5082114293- Epigenetics and DNA Methylation
- Forensic and Genetic Research
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- Environmental DNA in Biodiversity Studies
- Pharmacogenetics and Drug Metabolism
- Mitochondrial Function and Pathology
- Sex work and related issues
- Genetic diversity and population structure
- Metabolism and Genetic Disorders
- Poisoning and overdose treatments
- Identification and Quantification in Food
- Analytical Chemistry and Chromatography
- Race, Genetics, and Society
- Microfluidic and Capillary Electrophoresis Applications
- Genetic Syndromes and Imprinting
- Computational Drug Discovery Methods
- Autopsy Techniques and Outcomes
- Cancer-related molecular mechanisms research
- Cancer Treatment and Pharmacology
- Connective tissue disorders research
- Genetic Associations and Epidemiology
- Analytical Methods in Pharmaceuticals
- AI and HR Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
University Medical Center Freiburg
2012-2024
University of Freiburg
2014-2024
University of Amsterdam
2017-2018
Netherlands Institute for Neuroscience
2017-2018
The use of DNA methylation (DNAm) to obtain additional information in forensic investigations showed be a promising and increasing field interest. Prediction the chronological age based on age-dependent changes DNAm specific CpG sites within genome is one such potential application. Here we present an age-prediction tool for whole blood massive parallel sequencing (MPS) random forest machine learning algorithm. MPS allows accurate determination pre-selected markers neighboring CpG-sites...
The maternal mode of mitochondrial DNA (mtDNA) inheritance is central to human genetics. Recently, evidence for bi-parental mtDNA was claimed individuals three pedigrees that suffered disorders. We sequenced using both direct Sanger and Massively Parallel Sequencing in several tissues eleven maternally related other affiliated healthy a family pedigree observed mixed mitotypes eight individuals. Cells without nuclear DNA, i.e. thrombocytes hair shafts, only showed the mitotype haplogroup...
In this study, the authors developed a phenotyping method for CYP1A2, 2C9, 2C19, 2D6, and 3A4 using cocktail of 100 mg caffeine, 125 tolbutamide, 20 omeprazole, 30 dextromethorphan, 2 midazolam. A simple sampling scheme was established collecting 3 blood samples at 0, 4, 24 hours followed by solid-phase extraction liquid chromatography/tandem mass spectrometry analysis. After addition 8 deuterated internal standards extraction, analytes were separated gradient elution with ammonium acetate...
Nested PCR remains a labor-intensive and error-prone biomolecular analysis. Laboratory workflow automation by precise control of minute liquid volumes in centrifugal microfluidic Lab-on-a-Chip systems holds great potential for such applications. However, the majority these require costly custom-made processing devices. Our idea is to augment standard laboratory device, here real-time thermocycler, with inbuilt handling capabilities automation. We have developed disk segment enabling an...
The cheap and easy identification of species is necessary within multiple fields molecular biology. use high-resolution melting (HRM) DNA provides a fast closed-tube method for analysis the sequence composition mitochondrial genes 12S rRNA cytochrome b. We investigated potential HRM eleven different animal groups commonly found in Europe by animal-group-specific amplification followed melting. Influence factors as amount, additional single base alterations, existence mixed samples were taken...
Clozapine, a second generation antipsychotic which is relatively safe in overdose, has been used as an effective treatment alternative to traditional antipsychotics. The therapeutic use children remains controversial. However, accordance with the increasing prescription adults, accidental ingestion childhood becomes more frequent. We report youngest case of clozapine ingestion.A 13-month-old girl presented acute respiratory insufficiency and coma unknown origin. medical history, laboratory...
Throughout the last four centuries, many anatomical museums across world have collected teratological specimens that became precious objects. These can be regarded as spirits of past which captured morphology diseases through time. valuable and irreplaceable perfectly used in contemporary dysmorphological or genetic research. Unfortunately, due to historical nature these regularly aggressive preservation fluids, DNA degradation is often present. Furthermore, use material for extraction...
Abstract The analysis of age-dependent DNA methylation changes is a valuable tool in epigenetic research and forensic genetics. With some exceptions, most studies the past concentrated on blood, buccal, saliva samples. Another important sample type investigations hair, where has not been investigated so far. In this pilot study deeper look was taken at possibilities challenges hair. selected 5’-C-phosphate-G‑3’ (CpG) sites were characterized for their potential use as biomarker age...
Mitochondrial DNA is a widely tested genetic marker in various fields of research and diagnostics. Nonetheless, there still little understanding on its abundance quality within different tissues. Aiming to obtain deeper knowledge about the content mtDNA, we investigated nine tissues including blood, bone, brain, hair (root shaft), cardiac muscle, liver, lung, skeletal buccal mucosa 32 deceased individuals using two real-time quantitative PCR-based assays with differently sized mtDNA nDNA...