A5016280245- Epigenetics and DNA Methylation
- Forensic and Genetic Research
- Molecular Biology Techniques and Applications
- Genetic Syndromes and Imprinting
- RNA modifications and cancer
- Cancer-related gene regulation
- Environmental DNA in Biodiversity Studies
- Diatoms and Algae Research
- Cancer-related molecular mechanisms research
- Demographic Trends and Gender Preferences
- Salivary Gland Disorders and Functions
- Genetic Associations and Epidemiology
- Gut microbiota and health
- Gene expression and cancer classification
- Diet and metabolism studies
- Oral microbiology and periodontitis research
- Tissue Engineering and Regenerative Medicine
- Genomics and Chromatin Dynamics
- Statistical Methods and Inference
- Statistical Methods and Bayesian Inference
- Race, Genetics, and Society
- Forensic Entomology and Diptera Studies
- Chemical Synthesis and Characterization
- Electrochemical Analysis and Applications
- CRISPR and Genetic Engineering
Erasmus MC
2016-2025
University Medical Center
2024-2025
Maastricht University
2024-2025
King's College London
2013-2017
Queen Mary University of London
2012
The University of Texas MD Anderson Cancer Center
2007
The ability to estimate the age of donor from recovered biological material at a crime scene can be substantial value in forensic investigations. Aging complex and is associated with various molecular modifications cells that accumulate over person's lifetime including epigenetic patterns. aim this study was use age-specific DNA methylation patterns generate an accurate model for prediction chronological using data whole blood. In total, 45 age-associated CpG sites were selected based on...
Abstract Background Human DNA methylation profiling offers great promises in various biomedical applications, including ageing, cancer and even forensics. So far, most techniques are based on a chemical process called sodium bisulfite conversion, which specifically converts non-methylated cytosines into uracils. However, despite the popularity of this approach, it is known to cause fragmentation loss affecting standardization, while incomplete conversion may result potential...
Abstract Background Bisulfite conversion (BC) has been the gold standard in DNA methylation profiling for decades. During this chemical process, non-methylated cytosines are converted into uracils, while methylated remain intact. Despite its popularity, BC major drawbacks when used sensitive applications with low-quality and -quantity samples, such as required large amount of input, caused fragmentation loss, resulting reduced sequence complexity. Lately, to account BC-related disadvantages...
Forensic DNA phenotyping is gaining interest as the number of applications increases within forensic genetics community. The possibility providing investigative leads in addition to conventional profiling for human identification provides new insights into otherwise "cold" police investigations. ability reporting on bio-geographical ancestry (BGA), appearance characteristics and age based obtained from a crime scene sample an unknown donor makes exploration such markers development methods...
The presence of specific body fluids at crime scenes could be linked with particular types crime, therefore attributing a DNA profile to tissue increase the evidential significance match suspect. Current methodologies such as tissue‐specific mRNA profiling are useful but drawbacks include low specificity and applicability degraded samples. In this study, potential 11 differentially methylated regions, initially identified following large‐scale methylation analysis whole blood, buccal cells...
Inferring a person's smoking habit and history from blood is relevant for complementing or replacing self-reports in epidemiological public health research, forensic applications. However, finite DNA methylation marker set validated statistical model based on large dataset are not yet available. Employing 14 epigenome-wide association studies discovery, using data six population-based cohorts (N = 3764) building, we identified 13 CpGs most suitable inferring versus non-smoking status with...
Over the last few years, advances in massively parallel sequencing technologies (also referred to next generation sequencing) and bioinformatics analysis tools have boosted our knowledge on human microbiome. Such insights brought new perspectives possibilities apply microbiome many areas, particularly medicine. In forensic field, use of microbial DNA obtained from materials is still its infancy but has been suggested as a potential alternative situations when other (non-microbial) approaches...
Information on the time when a stain was deposited at crime scene can be valuable in forensic investigations. It link DNA-identified donor with or provide post-mortem interval estimation cases cadavers. The available methods for estimating deposition have limitations of different types and magnitudes. In this proof-of-principle study we investigated first use microbial DNA purpose human saliva stains. First, identified most abundant frequent bacterial species using publicly 16S rRNA gene...
Abstract Background Although the genomes of monozygotic twins are practically identical, their methylomes may evolve divergently throughout lifetime as a consequence factors such environment or aging. Particularly for young and healthy twins, DNA methylation divergence, if any, be restricted to stochastic processes occurring post-twinning during embryonic development early life. However, what extent mechanisms can systematically provide stable source inter-individual epigenetic variation...
Background: Epigenetic biomarkers, particularly CpG methylation, are increasingly employed in clinical and forensic settings. However, we still lack a cost-effective, sensitive, medium-scale method for the analysis of hundreds to thousands user-defined CpGs suitable minute DNA input amounts (<10 ng). In this study, motivated by promising results genetics field, investigated single-molecule molecular inversion probes (smMIPs) simultaneous using an example set 514 age-associated (Zhang...
<title>Abstract</title> <bold>Background: </bold>The HumanMethylationEPIC v2.0 BeadChip (EPIC v2.0) microarray is a widely used tool for genome-wide DNA methylation (DNAm) analysis, designed high-quality human with recommended input of 250 ng. However, in clinical and forensic settings, samples may be low quality and/or quantity (highly fragmented available amounts). This study assessed the performance EPIC on various combinations average fragment size (350, 230, 165, 95 bp) amount (100, 50,...