A5059513645- Forensic and Genetic Research
- Molecular Biology Techniques and Applications
- Genetic diversity and population structure
- Race, Genetics, and Society
- Genetic Associations and Epidemiology
- Environmental DNA in Biodiversity Studies
- Forensic Anthropology and Bioarchaeology Studies
- Demographic Trends and Gender Preferences
- melanin and skin pigmentation
- Epigenetics and DNA Methylation
- Identification and Quantification in Food
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Phylogenetic Studies
- Homicide, Infanticide, and Child Abuse
- Milk Quality and Mastitis in Dairy Cows
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Health, Environment, Cognitive Aging
- Genetic Mapping and Diversity in Plants and Animals
- Genetic Syndromes and Imprinting
- Herpesvirus Infections and Treatments
- Animal testing and alternatives
- Cancer-related molecular mechanisms research
- Intergenerational Family Dynamics and Caregiving
- RNA regulation and disease
Erasmus MC
2016-2025
Erasmus University Rotterdam
2013-2018
DDL Diagnostic Laboratory
2015
Recently, the field of predicting phenotypes externally visible characteristics (EVCs) from DNA genotypes with final aim concentrating police investigations to find persons completely unknown investigating authorities, also referred as Forensic Phenotyping (FDP), has started become established in forensic biology. We previously developed and forensically validated IrisPlex system for accurate prediction blue brown eye colour DNA, recently showed that all major hair categories are predictable...
Relevant for various areas of human genetics, Y-chromosomal short tandem repeats (Y-STRs) are commonly used testing close paternal relationships among individuals and populations, male lineage identification. However, even the widely 17-loci Yfiler set cannot resolve populations completely. Here, 52 centers generated quality-controlled data 13 rapidly mutating (RM) Y-STRs in 14,644 related unrelated males from 111 worldwide populations. Strikingly, >99% 12,272 were completely individualized....
Next-generation sequencing (NGS) technologies offer immense possibilities given the large genomic data they simultaneously deliver. The human Y-chromosome serves as good example how NGS benefits various applications in evolution, anthropology, genealogy, and forensics. Prior to NGS, phylogenetic tree consisted of a few hundred branches, based on data, it now contains many thousands. complexity both, Y provide challenges for haplogroup assignment. For effective analysis interpretation we...
Whole mitochondrial (mt) genome analysis enables a considerable increase in throughput, and improves the discriminatory power to maximum possible phylogenetic resolution. Most established protocols on different massively parallel sequencing (MPS) platforms, however, invariably involve PCR amplification of large fragments, typically several kilobases size, which may fail due mtDNA fragmentation available degraded materials. We introduce MPS tiling approach for simultaneous whole human mt...
Short tandem repeat polymorphisms on the male-specific part of human Y-chromosome (Y-STRs) are valuable tools in many areas genetics. Although their paternal inheritance and moderate mutation rate (~10−3 mutations per marker meiosis) allow detecting relationships, they typically fail to separate male relatives. Previously, we identified 13 Y-STR markers with untypically high rates (>10−2), termed rapidly mutating (RM) Y-STRs, showed that improved relative differentiation over standard...
Responding to the growing scientific and practical interest in forensic DNA phenotyping, VISible Attributes through GEnomics (VISAGE) Consortium was founded 2017 with main goal of developing validating new reliable molecular statistical tools predict appearance, ancestry age from DNA. Here, we describe development inter-laboratory evaluation validation VISAGE Enhanced Tool for Appearance Ancestry inference The is first forensic-driven genetic laboratory tool that comprises well-established...
The VISAGE Enhanced Tool for Appearance and Ancestry (ET) has been designed to combine markers the prediction of bio-geographical ancestry plus a range externally visible characteristics into single massively parallel sequencing (MPS) assay. We describe development panel used in ET, enhanced analyses they provide compared previous MPS-based forensic assays. As well as established autosomal nucleotide polymorphisms (SNPs) that differentiate sub-Saharan African, European, East Asian, South...
SNPs from the non-recombining part of human Y chromosome (Y-SNPs) are informative to classify paternal lineages in forensic, genealogical, anthropological, and evolutionary studies. Although thousands Y-SNPs were identified thus far, previous Y-SNP multiplex tools target only dozens markers simultaneously, thereby restricting provided Y-haplogroup resolution limiting their applications. Here, we overcome this shortcoming by introducing a high-resolution tool for parallel...
The Y chromosome is paternally inherited and therefore serves as an evolutionary marker of patrilineal descent. Worldwide DNA variation within the non-recombining portion can be represented a monophyletic phylogenetic tree in which branches (haplogroups) are defined by at least one SNP. Previous human population genetics research has produced wealth knowledge about worldwide distribution Y-SNP haplogroups. Here, we apply previous very recent on phylogeny Y-haplogroup introducing two...
The discovery of rapidly mutating (RM) Y-STRs started to move the field forensic Y-STR analysis from male lineage identification towards individual identification. Previously, value RM for differentiating relatives was limited due modest number 13 identified Y-STRs. Recently, new were discovered, with strong expectations significantly improving relative differentiation; however, empirical evidence is missing yet. More recently, genotyping method RMplex efficiently analyzing 30 high mutation...
The principal limitation of forensic Y-STR analysis, which identifies a male lineage rather than an individual man, is being addressed by the discovery and application rapidly mutating Y-STRs (RM Y-STRs). Due to their higher mutation rates compared standard used in forensics, RM significantly enhance ability differentiate between relatives. However, some relatives - particularly closely related ones remain indistinguishable. Given design execution two previous searches that discovered 26...
Abstract Y-chromosomal short tandem repeat (Y-STR) markers are routinely used in forensic casework to identify male donors of biological traces left at crime scenes, particularly sexual assault cases. However, the evidential value a match between Y-STR profile trace and potential donor, usually suspect, is difficult quantify, common albeit inappropriate practise equate probabilities with frequencies estimated from population databases has been subject scientific debate for decades. As...
Abstract For decades, there has been scientific interest in the variation and geographic distribution of paternal lineages associated with human Y chromosome. However, relevant data have dispersed across numerous publications, making it difficult to consolidate. Additionally, understanding relationships between different variants, tools used analyze them, evolved over time, further complicating efforts harmonize this information. The Universal Y-SNP Database (UYSD) marks a substantial...