A5028886987- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- COVID-19 and Mental Health
- Disaster Response and Management
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Hepatitis Viruses Studies and Epidemiology
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Invertebrate Immune Response Mechanisms
- Viral gastroenteritis research and epidemiology
- Cancer Cells and Metastasis
- Sepsis Diagnosis and Treatment
- Travel-related health issues
- Click Chemistry and Applications
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- Biotin and Related Studies
- School Health and Nursing Education
Hong Kong Polytechnic University
2024
Chinese University of Hong Kong
1999-2024
Prince of Wales Hospital
2003
Manipulation of cell–cell interactions via cell surface modification is crucial in tissue engineering and cell-based therapy. To be able to monitor intercellular interactions, it can also provide useful information for understanding how the cells interact communicate. We report herein a facile bioorthogonal strategy promote interactions. It involves use maleimide-appended tetrazine-caged boron dipyrromethene (BODIPY)-based fluorescent probe maleimide-substituted bicyclo[6.1.0]non-4-yne (BCN)...
Chemo-resistance hinders treatment of patients with hepatocellular carcinoma. Although there are many models that can be found in the literature, root mechanism to explain chemo-resistance is still not fully understood. To gain a better understanding this phenomenon, chemo-resistant line, R-HepG2, was developed from chemo-sensitive HepG2 line through an exposure doxorubicin (DOX). The R-HepG2 exhibited cancer stem cell (CSC) phenotype over-expression P-glycoprotein (P-gp), conferring it...
The Drosophila imaginal disc has been an excellent model for the study of developmental gene regulation. In particular, long non-coding RNAs (lncRNAs) have gained widespread attention in recent years due to their important role Their specific spatiotemporal expressions further support processes and diseases. this study, we explored a novel lncRNA leg development by dissecting dissociating w1118 third-instar larval third (L3) discs into single cells nuclei, performing single-cell...
Characterization of the subcellular distribution RNA is essential for understanding molecular basis biological processes. Here, nanopore direct RNA-sequencing (DRS) four lung cancer cell lines (A549, H1975, H358, and HCC4006) performed, coupled with a computational pipeline, Low-abundance Aware Full-length Isoform clusTEr (LAFITE), to comprehensively analyze full-length cytoplasmic nuclear transcriptome. Using additional DRS orthogonal data sets, it shown that LAFITE outperforms current...
Three-dimensional (3D) spheroid models are crucial for cancer research, offering more accurate insights into tumour biology and drug responses than traditional 2D cell cultures. However, inconsistent low-throughput production has hindered their application in screening. Here, we present an automated high-throughput platform a selection, fabrication, sorting system (SFSS) to produce uniform gelatine-encapsulated spheroids (GESs) with high efficiency. SFSS integrates advanced imaging,...