A5007788682- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Silk-based biomaterials and applications
- Cancer Immunotherapy and Biomarkers
- Galectins and Cancer Biology
- Blood disorders and treatments
- interferon and immune responses
- NF-κB Signaling Pathways
- Protist diversity and phylogeny
- Insect and Arachnid Ecology and Behavior
- Viral Infections and Vectors
- Cancer-related gene regulation
- Immunodeficiency and Autoimmune Disorders
- Nanoplatforms for cancer theranostics
- Cancer, Hypoxia, and Metabolism
- Photodynamic Therapy Research Studies
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Erythrocyte Function and Pathophysiology
- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Outbreaks Research
City University of Hong Kong
2018-2025
City University of Hong Kong, Shenzhen Research Institute
2023
University of Washington
2015-2018
University of California, Berkeley
2011-2013
Children's Hospital
2012
Dana-Farber Cancer Institute
2007-2012
Boston Children's Museum
2012
Harvard University
2007-2009
Pattern recognition receptors (PRRs) survey intra- and extracellular spaces for pathogen-associated molecular patterns (PAMPs) within microbial products of infection. Recognition binding to cognate PAMP ligand by specific PRRs initiates signaling cascades that culminate in a coordinated intracellular innate immune response designed control In particular, our system has evolved specialized discriminate viral nucleic acid from host. These are critical sensors RNA trigger immunity the...
Photodynamic therapy (PDT) utilizes reactive oxygen species (ROS) for eradication of cancer cells. Its effectiveness is governed by the content, which scarce in hypoxic tumor microenvironment. We report herein two zinc(II) phthalocyanines substituted with or four nitric oxide (NO)-releasing moieties, namely ZnPc-2NO and ZnPc-4NO, can suppress mitochondrial respiration, thereby sparing more intracellular PDT. Using HT29 human colorectal adenocarcinoma cells A549 lung carcinoma cells, we have...
Manipulation of cell–cell interactions via cell surface modification is crucial in tissue engineering and cell-based therapy. To be able to monitor intercellular interactions, it can also provide useful information for understanding how the cells interact communicate. We report herein a facile bioorthogonal strategy promote interactions. It involves use maleimide-appended tetrazine-caged boron dipyrromethene (BODIPY)-based fluorescent probe maleimide-substituted bicyclo[6.1.0]non-4-yne (BCN)...
Background The presence of tumor-specific mutations in the cancer genome represents a potential opportunity for pharmacologic intervention to therapeutic benefit. Unfortunately, many classes oncoproteins (e.g., transcription factors) are not amenable conventional small-molecule screening. Despite identification somatic mutations, most therapy still utilizes nonspecific, cytotoxic drugs. One illustrative example is treatment Ewing sarcoma. Although EWS/FLI oncoprotein, present vast majority...
Acute myeloid leukemia (AML) is the most common form of acute in adults. Long-term survival patients with AML has changed little over past decade, necessitating identification and validation new targets. Integration genomic approaches small-molecule genetically based high-throughput screening holds promise improved discovery candidate targets for cancer therapy. Here, we identified a role glycogen synthase kinase 3α (GSK-3α) by performing 2 independent library screens an shRNA screen...
Site-selective acetylation of a single lysine residue in protein that reaches acetyltransferase's accuracy, precision, and reliability is challenging. Here, we report peptide-guided, proximity-driven group transfer reaction acetylates residue, Lys 248, the fragment crystallizable region (Fc region) heavy chain human Immunoglobulin G (IgG). An Fc-interacting peptide bound with Fc domain positioned phenolic ester close to which induced nucleophilic resulted an acetyl 248. The proceeded decent...
ABSTRACT Induction of interferon beta (IFN-β), IFN-stimulated genes (ISGs), and inflammatory responses is critical for control viral infection. We recently identified an essential linkage stimulation the cytokine interleukin-1β (IL-1β) induction ISGs that function as host restriction pathways against emerging flavivirus West Nile virus (WNV) in vivo . Here we utilized ex global transcriptome analysis primary dendritic cells, known targets WNV replication, to define gene signatures required...
Precise regulation of Rag (recombination-activating gene) expression is crucial to prevent genomic instability caused by the generation Rag-mediated DNA breaks. Although mechanisms activation have been well characterized, mechanism which down-regulated in early B cell development has not fully elucidated. Using a complementary library screen, we identified transcriptional repressor Gfi1b as negative regulator locus. Expression causes repression Rag1 and Rag2 lines primary mouse cells....
Foxo1 is a critical, direct regulator of Rag (recombination activating gene) transcription during B cell development and thus essential for the generation diverse repertoire antigen receptors. Although regulation has been widely studied in many types, pathways regulating cells have not fully elucidated. By screening panel mutants, we identified serine 215 on as novel phosphorylation site that activation transcription. Mutation S215 strongly attenuated transactivation but did affect most...
Given the extremely limited regeneration potential of heart, one most effective strategies to reduce prevalence and mortality coronary artery disease is prevention. Short-chain fatty acids (SCFAs), which are by-products beneficial probiotics, have been reported possess cardioprotective effects. Despite their roles, delivering SCFAs maintaining concentration in plasma present major challenges. Therefore, study, we aimed devise a strategy prevent heart effectively by using engineered...
Abstract We examined the signaling pathways and cell type–specific responses of IFN regulatory factor (IRF) 5, an immune-regulatory transcription factor. show that protein kinases IKKα, IKKβ, IKKε, TANK-binding kinase 1 each confer IRF5 phosphorylation/dimerization, thus extending family activator kinases. Among primary human immune subsets, we found is most abundant in plasmacytoid dendritic cells (pDCs). Flow cytometric imaging revealed specifically activated by endosomal TLR signaling....
Abstract Plasmacytoid dendritic cells are a rare subset of that exhibit antiviral functions in response to toll-like receptor 7/8 stimulations. Alternative receptors such as TLR4 have been known be active plasmacytoid for immune regulatory functions. However, it is unclear whether these differentially activate compared with canonical stimulation. Here, we assessed alternative cell activation states mediated by other than endosomal via the RNA sequencing approach. We found 4 stimulation...
Abstract Pathogen recognition receptor (PRR) signaling is critical for triggering innate immune activation and the expression of response genes, including genes that impart restriction against virus replication. RIG-I-like receptors TLRs are PRRs signal drive antiviral production type I IFN leading to induction IFN-stimulated in part through interferon regulatory factor (IRF) family transcription factors. Previous studies with West Nile (WNV) showed IRF3 IRF7 regulate fibroblasts neurons,...
Growth factor independence genes (Gfi1 and Gfi1b) repress recombination activating (Rag) transcription in developing B lymphocytes. Because all blood lineages originate from hematopoietic stem cells (HSCs) different lineage progenitors have been shown to share networks prior cell fate commitment, we hypothesized that GFI family proteins may also play a role repressing Rag or global lymphoid transcriptional program other lineages. We tested the level of various when Gfi1 Gfi1b were deleted,...
Abstract Photodynamic therapy (PDT) utilizes reactive oxygen species (ROS) for eradication of cancer cells. Its effectiveness is governed by the content, which scarce in hypoxic tumor microenvironment. We report herein two zinc(II) phthalocyanines substituted with or four nitric oxide (NO)‐releasing moieties, namely ZnPc‐2NO and ZnPc‐4NO , can suppress mitochondrial respiration, thereby sparing more intracellular PDT. Using HT29 human colorectal adenocarcinoma cells A549 lung carcinoma...
<title>Abstract</title> Cell chirality is an intrinsic property shown as biased cell rotation or orientation. While the righthanded helix of actin known essential, it intriguing how single form molecular handedness can be manifested into diverse and even mirrored forms chirality. Here, we found that nucleus rotates with clockwise (CW) bias smaller projected area but reverses to anticlockwise (ACW) when spreading increases. Actin analysis suggests polymerization radial fibers accounts for ACW...
Cell chirality is an intrinsic property shown as biased cell rotation or orientation. Although the right-handed double helix of actin known important, how a single form molecular handedness manifests diverse forms remains unclear. Here, we found that nucleus rotated with clockwise (CW) bias in small projected area, but this reversed to anticlockwise (ACW) spreading increased. Actin analysis suggested radial fiber polymerization accounts for ACW bias. Alterations transverse arc components...
Interferon regulatory factor 5 (IRF5) is a key transcription for regulating the immune response. It activated downstream of Toll-like receptor myeloid differentiation primary response gene 88 (TLR-MyD88) signaling pathway. IRF5 activation involves phosphorylation, dimerization, and subsequent translocation from cytoplasm into nucleus, which in turn induces expression various pro-inflammatory cytokines. A detection assay essential to studying functions its relevant pathways. This article...