A5036489000- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Hematopoietic Stem Cell Transplantation
- RNA Interference and Gene Delivery
- Renal and related cancers
- Acute Lymphoblastic Leukemia research
- Chronic Lymphocytic Leukemia Research
- Eosinophilic Disorders and Syndromes
- Hematological disorders and diagnostics
- interferon and immune responses
- Lymphoma Diagnosis and Treatment
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Platelet Disorders and Treatments
- Hemoglobinopathies and Related Disorders
- Neutropenia and Cancer Infections
- Mesenchymal stem cell research
Niigata University
2014-2025
RMIT University
2001-2014
Toranomon Hospital
2011
Abstract Purpose: To investigate the safety and immunologic clinical responses of dendritic cell therapy for patients with recurrent malignant glioma. Experimental Design: Twenty-four glioma (6 grade 3 18 4 patients) were evaluated in a phase I/II study therapy. All resistant to standard maximum The patient's peripheral blood cells generated granulocyte macrophage colony-stimulating factor, plus interleukin or without OK-432, pulsed an autologous tumor lysate. Dendritic injected...
In this Phase I/II trial, the patient's peripheral blood dendritic cells were pulsed with an autologous tumour lysate of glioma. Seven patients glioblastoma and three anaplastic glioma, ranging in age from 20 to 69 years, participated study. The mean numbers vaccinations lysate-pulsed 3.7 times intradermally close a cervical lymph node, 3.2 intratumorally via Ommaya reservoir. percentage CD56-positive lymphocytes increased after immunisation. There two minor responses four no-change cases...
Patients with advanced stage of squamous cell carcinoma esophagus have a poor prognosis lethal outcome. In order to explore the feasibility and effectiveness dendritic (DC)‑based immunotherapy for esophagus, we performed phase Ⅰ/Ⅱ clinical trial monocyte‑derived cells (moDCs) pulsed SART1 peptide in seven patients this disease. Although therapy was definite, not clearly confirmed esophagus. However, vitro study revealed that moDCs generated possessed potent ability inducing peptide‑specific...
Abstract Although allogeneic hematopoietic stem cell transplantation (allo‐HSCT) is a curative therapy for high‐risk acute leukemia (AL), some patients still relapse. Since simultaneously have many prognostic factors, difficulties are associated with the construction of patient‐based prediction algorithm The alternating decision tree (ADTree) successful classification method that combines trees predictive accuracy boosting. It component machine learning (ML) and has capacity to analyze...
Primary familial and congenital polycythaemia (PFCP) is a rare disease characterized by erythrocytosis inherited in an autosomal dominant fashion. Recently, mutations the erythropoietin receptor (EpoR) have been identified PFCP families. We describe Japanese family with inheritance of PFCP. An vitro colony assay demonstrated hypersensitivity erythroid progenitors to (Epo) affected members. Sequence analysis RT‐PCR products amplified from C‐terminal region EpoR transcripts members revealed...
We demonstrate that exposure to the AB5 subtilase cytotoxin (SubAB) induces unfolded protein response (UPR) in human peripheral blood mononuclear cells, concomitant with a proinflammatory across distinct cell subsets. Notably, SubAB selectively type-I interferon (IFN) expression plasmacytoid dendritic acting synergistically Toll-like receptor 7 stimulation. The induction of IFN relies on stimulator genes (STING) activation, coupled synthesis inhibition mediated by kinase R-like endoplasmic...
Although dendritic cells (DC) can be cultured from cord blood (CB) CD34+ progenitor cells, the generation of DC CB monocytes has not been reported. In this paper, we explored to establish simplest way obtain a substantial number CB. We isolated mononuclear (CB-MNC) by plastic adherence method. These adherent (monocyte-rich cells) were in RPMI 1640 medium supplemented with 10 % fetal bovine serum (FBS) or serum-free X-VIVO 15 (SFM) for 7 days, both which contained 100 ng/ml...
Abstract How to treat CML patients who are resistant inhibitors of BCR‐ABL tyrosine kinase such as Imatinib is a very important and urgent issue in clinical hematology. Here, we report case Imatinib‐treated which intradermally administered WT1 peptide vaccine elicited WT1‐specific immune responses the resultant reduction persistent residual disease co‐administration Imatinib. mRNA levels were being maintained under detection limit for 8 months since week 77 vaccination. No adverse effects...
Mesenchymal stromal cells (MSCs) are known to have immunosuppressive ability and been used in clinical treatment of acute graft- versus -host disease, one severe complications the hematopoietic stem cell transplantation. However, MSCs activated suppress immune system only after encountering an inflammatory stimulation. Thus, it will be ideal if primed ready reaction before being administered. Triptolide (TPL) is a diterpene triepoxide purified from Chinese herb-Tripterygium wilfordii Hook.f....
Although tyrosine kinase inhibitors is effective for dramatically reducing CML cells, it might be difficult to eradicate completely the stem cells.We aimed clarify safety and effects of WT1 peptide vaccination in combination with imatinib therapy a patient.A 51 year-old male CP, who showed resistance against 2.5 years, began treated 9mer modified-type peptides standard dose imatinib.Although every 2-week-administration 22 weeks did not show definite on quantification bcr-abl transcripts, by...
Although the roles of each low-frequency immunocompetent cells such as dendritic (DCs), γδT cells, and Treg in induction acute or chronic graft versus host disease (GVHD) have been discussed several reports, there are few papers dealing with an evaluation these together simultaneously patients hematopoietic stem cell transplantation (HSCT) explored kinetics association GVHD.In present study, we assessed number plasmacytoid DCs (pDCs), myeloid (mDCs), serially who received allogeneic HSCT...
Chronic myelogenous leukemia is caused by the acquisition of reciprocal (9;22)(q34;q11) chromosomal translocation in hematopoietic stem cells. The fusion protein showed higher and aberrant tyrosine kinase activity. inhibition activity represents a specific therapeutic strategy for bcr/abl-expressing leukemias. STI571 compound 2-phenylaminopyrimidine class that selectively inhibits Abl kinase. In this study, we evaluated effects on antigen presentation dendritic cells generated from patients...
Although expansions in gamma delta T cell populations are known to occur the peripheral blood of patients infected with Plasmodium falciparum, role these cells people naturally acquired immunity against P. falciparum who live malaria-endemic areas is poorly understood. We used a cross-sectional survey investigate living Lao People's Democratic Republic, area. found that proportion non-Vgamma9 was higher non-hospitalized uncomplicated malaria (UMPs) from this region. Notably, we UMPs and...
Abstract We examined the signaling pathways and cell type–specific responses of IFN regulatory factor (IRF) 5, an immune-regulatory transcription factor. show that protein kinases IKKα, IKKβ, IKKε, TANK-binding kinase 1 each confer IRF5 phosphorylation/dimerization, thus extending family activator kinases. Among primary human immune subsets, we found is most abundant in plasmacytoid dendritic cells (pDCs). Flow cytometric imaging revealed specifically activated by endosomal TLR signaling....
Lymphocytosis predominantly due to natural killer (NK) cells has been reported in nearly a half of chronic myelogenous leukemia (CML) patients who were being treated with dasatinib. Besides, dasatinib-treated lymphocytosis have better prognosis than without lymphocytosis. In order elucidate the effects dasatinib on proliferation lymphocyte subset, was added culture peripheral blood mononuclear IL-2 (lymphokine-activated culture) or low dose zoledronate (γδ T-cell culture). both conditions,...