A5011459023- Liver physiology and pathology
- Angiogenesis and VEGF in Cancer
- Advanced Fluorescence Microscopy Techniques
- Peroxisome Proliferator-Activated Receptors
- Neonatal Health and Biochemistry
- Organ Transplantation Techniques and Outcomes
- Pediatric Hepatobiliary Diseases and Treatments
- Pluripotent Stem Cells Research
- Metabolism and Genetic Disorders
- Click Chemistry and Applications
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Heme Oxygenase-1 and Carbon Monoxide
- Adipose Tissue and Metabolism
- Erythrocyte Function and Pathophysiology
- Kruppel-like factors research
- Optical properties and cooling technologies in crystalline materials
- Retinal Diseases and Treatments
- Receptor Mechanisms and Signaling
- SARS-CoV-2 and COVID-19 Research
- Liver Disease Diagnosis and Treatment
- Luminescence Properties of Advanced Materials
- Extracellular vesicles in disease
- Congenital heart defects research
- Genomics and Chromatin Dynamics
Spanish National Centre for Cardiovascular Research
2005-2022
Instituto de Salud Carlos III
2005
Universidad Complutense de Madrid
2000-2001
Mitochondrial production of oxidants contributes to a variety pathological conditions including the vascular complications diabetes, neurodegenerative diseases, and cellular senescence. We postulated that transcriptional coactivator, peroxisome proliferator activated receptor-gamma coactivator 1alpha (PGC-1alpha), major regulator oxidative metabolism mitochondrial biogenesis, could be involved in regulation antioxidant defense system endothelial cells.We show PGC-1alpha is present human,...
Abstract Introduction The mammalian adult heart maintains a continuous, low cardiomyocyte turnover rate throughout life. Although many cardiac stem cell populations have been studied, the natural source for homeostatic repair has not yet defined. Polycomb protein BMI1 is most representative marker of mouse systems. We evaluated relevance and role Bmi1 + cells in physiological homeostasis. Methods CreER/+ ; Rosa26 YFP/+ ( -YFP) mice were used lineage tracing strategy. After tamoxifen (TM)...
The nucleocapsid (N) protein is the only phosphorylated structural of coronavirus Transmissible gastroenteritis virus (TGEV). phosphorylation state and intracellular distribution TGEV N in infected cells were characterized by a combination techniques including: (i) subcellular fractionation analysis tryptic peptides two-dimensional nano-liquid chromatography, coupled to ion-trap mass spectrometry; (ii) tandem mass-spectrometry resolved SDS-PAGE; (iii) Western blotting using two specific...
ABSTRACT Number of molecules and Brightness (N&B) has been proposed for measuring the molecular brightness number fluorophores in time‐sequence images, live cells. If fluorescently tagged‐proteins are mobile illumination volume, stoichiometry their oligomers can be derived from increase fluorescent dyes due to clustering. We examine aspects concerning extra‐fluctuation effects induced by cell shifts photobleaching, which yield large overestimates clusters size sub‐unit counts. develop an...
In a previous study, we demonstrated that endothelial microvesicles (eMVs) have well‐developed enzymatic team involved in reactive oxygen species detoxification. the present paper, demonstrate eMVs can synthesize reducing power (NAD(P)H) nourishes this team, especially those derived from senescent human umbilical vein cells. Moreover, molecules nourish machinery NAD(P)H synthesis are blood plasma metabolites: lactate, pyruvate, glucose, glycerol, and branched‐chain amino acids. Drastic...
The fusion of bone marrow (BM) hematopoietic cells with hepatocytes to generate BM derived (BMDH) is a natural process, which enhanced in damaged tissues. However, the reprogramming needed BMDH and identity resultant essentially unknown. In mouse model chronic liver damage, here we identify modification chromatin structure nucleus during formation, accompanied by loss key transcription factor PU.1/Sfpi1 (SFFV proviral integration 1) gain hepatic transcriptional regulator HNF-1A homeobox A...
Both fibroblast growth factor-2 (FGF2) and neural cell adhesion molecule (NCAM) trigger FGF receptor 1 (FGFR1) signaling, however they induce remarkably distinct trafficking cellular responses. The molecular basis of such a dichotomy the role types ligand-receptor interactions remain elusive. Number molecules Brightness (N&B) analysis revealed that FGF2 NCAM promote different FGFR1 assembly dynamics at plasma membrane. stimulation elicits long-lasting cycles short-lived monomers...
Yb and Er codoped NaT(XO4)2 (T = Y, La, Gd, Lu X Mo, W) disordered oxides show a green (Er3+ related) up-conversion (UC) efficiency comparable to that of Yb:Er:β-NaYF4 compound unless 3 times larger UC ratiometric thermal sensitivity. The similar Yb:Er doped β-NaYF4 compounds allowed testing equal subcutaneous depths ex-vivo chicken tissue in both cases. This extraordinary behavior for with large cutoff phonon energy (ħω≈ 920 cm-1) is ascribed 4F9/2 electron population recycling higher...
ABSTRACT Most studies addressing chromatin behaviour during preimplantation development are based on biochemical assays that lack spatial and cell-specific information, crucial early development. Here, we describe the changes in taking place at transition from totipotency to lineage specification, by using direct stochastical optical reconstruction microscopy (dSTORM) whole-mount embryos first stages of mouse Through study two post-translational modifications Histone 3 related active...
Despite the importance and ubiquity of receptor oligomerization, few methods are applicable for detecting clustering events measuring degree clustering. Here, we describe an imaging approach to determine average oligomeric state mEGFP-tagged-receptor homocomplexes in membrane living cells. The protocol is based on Total Internal Reflection Fluorescence (TIRF) microscopy combined with Number Brightness (N&B) analysis. N&B a method similar fluorescence-correlation spectroscopy (FCS) photon...
An analysis of liver cell populations from both adult and 21 day pregnancy rat fetuses (E21) was carried out. The results show that E21 hepatocytes express OX‐43, as do endothelial cells but not hepatocytes. OX‐43 could be used in future a marker for the hepatocyte maturation.
Despite the importance and ubiquity of receptor oligomerization, few methods are applicable for detecting clustering events measuring degree clustering. Here, we describe an imaging approach to determine average oligomeric state mEGFP-tagged-receptor homocomplexes in membrane living cells. The protocol is based on Total Internal Reflection Fluorescence (TIRF) microscopy combined with Number Brightness (N&B) analysis. N&B a method similar fluorescence-correlation spectroscopy (FCS) photon...