A5003000668- Parasitic Diseases Research and Treatment
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Vector-Borne Animal Diseases
- Protist diversity and phylogeny
- Health Systems, Economic Evaluations, Quality of Life
- Insect Utilization and Effects
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Animal Disease Management and Epidemiology
- Mosquito-borne diseases and control
- Chronic Disease Management Strategies
- Genomics and Phylogenetic Studies
- Insects and Parasite Interactions
- Lymphatic System and Diseases
University of Buea
2017-2024
Conventional diagnosis of filarial infections is based on morphological identification microfilariae using light microscopy and requires considerable expertise, time-consuming, can be subjective. Loop-mediated isothermal amplification (LAMP) has advantages over or PCR because its operational simplicity, rapidity versatility readout options. LAMP assays represent a major step forward in improved diagnostic tools suitable for low resource settings field applicability. The study goal was to...
Biting midges belonging to the genus Culicoides are tiny stout-shaped hematophagous insects and thought transmit filarial nematode Mansonella perstans. Little is known about fauna in rain forest belt of Littoral Region Cameroon. This study was designed investigate diversity, abundance distribution spp. their role as purported vector(s) M. Overnight light trap collections human landing catches (HLCs) revealed eight species with C. grahamii being most abundant followed by milnei. Four...
Approximately 114 million people are infected with Mansonella perstans in large proportions of Africa. In contrast to other filariae that infect humans, M. perstans-infected individuals show no distinct pathology or specific clinical picture, indicating a well-tuned adaptation the host. addition, since adult worms reside serous cavities which difficult access, research has been hindered and there is paucity knowledge about biology perstans, especially development different life stages as...
Culicoides (Diptera; Ceratoponidae) are tiny, stout, blood-sucking flies with a near worldwide distribution. When present, they often considered biting nuisance but in addition, involved the transmission of pathogens to humans, domestic and wild animals. Data on species South-West region Cameroon dates back 1950s. Over decades, ecological transformation due agriculture deforestation may have affected population dynamics therefore our study provides an update their bio-ecology region....
Background Infections with Onchocerca volvulus nematodes remain a threat in Sub-Saharan Africa after three decades of ivermectin mass drug administration. Despite this effort, there is still an urgent need for understanding the parasite biology especially mating behaviour and nodule formation as well development more potent drugs that can clear developmental (L3, L4, L5) adult stages inhibit reproduction behaviour. Methodology/Principal findings Prior to culture, freshly harvested O . L3...
Despite long-term mass drug administration programmes, approximately 220 million people are still infected with filariae in endemic regions. Several research studies have characterized host immune responses but a major obstacle for on human has been the inability to obtain adult worms which turn hindered analysis infection kinetics and signalling. Although Litomosoides sigmodontis filarial mouse model is well-established, complex immunological mechanisms associated control disease...
Endemic communities of Mansonella perstans infections have been neglected since associated pathology remains undefined. Consequently, improvements in drug therapy also ignored despite a large number infected individuals areas Cameroon. Thus, we established an vitro system to culture M. microfilariae (Mf); the transmission stage infection. In short, compared ability two renowned media (Dulbecco's Modified Eagle's Medium (DMEM) and Roswell Park Memorial Institute (RPMI-1640)) sustain Mf...
The 13,647-bp complete mitochondrial genome of Mansonella perstans was sequenced and is syntenic to the ozzardi . Phylogenetic analysis consistent with known phylogeny ONC5 group filarial nematodes.
Abstract Background Prior to carrying out clinical trials, it is important assess the health status of study participants be able interpret subsequent changes that may related effects treatments during follow-up patients. This presents clinical, haematological and biochemical profiles podoconiosis patients prior their involvement in PodoLEDoxy trial. Methods All lower limb lymphoedema visiting centre were screened a diagnosis was based on manifestation detailed medical history. Patients who...
Introduction Mouse models of human filarial infections are not only urgently needed to investigate the biology nematodes and their modulation host’s immunity, but will also provide a platform screen test novel anti-filarial drugs. Recently, murine Loa loa infection have been stablished using immunocompromised mouse strains, whereas Mansonella perstans implemented until now. Methods Therefore, we aim establish experimental M. strains RAG2IL-2Rγ -/- (lack B, T natural killer cells),...
Mansonella perstans infections are widespread in Sub-Saharan Africa and Central South America thus can be considered as the most prevalent parasite of man tropical Africa. In contrast to high prevalence, knowledge about biology this filarial nematode is restricted no effective treatment regimens ivermectin-resistant lacking. An obstacle for research that M. resides body cavities have been only rarely recovered during surgery or autopsy. Therefore, alternative methods like vitro culture...
Background Mansonella perstans is transmitted by Culicoides species and affects hundred millions of inhabitants in about 33 countries sub-Saharan Africa. It known that Mansonellosis due to do not result a clear clinical picture, but down-regulates the immunity patients predisposing them other diseases like tuberculosis, HIV malaria or damping vaccine efficacy. However, research novel drugs against this filarial nematode missing because lack parasite material. Previous studies have developed...
Abstract Background Infections with Onchocerca volvulus nematodes remain a threat in Sub-Saharan Africa after two decades of ivermectin mass drug administration. Despite this effort, there is still an urgent need for understanding the parasite biology, especially mating behaviour and nodule formation, as well development more potent drugs that can clear developmental (L3, L4, L5) adult stages inhibit parasite’s reproductive behavioural pattern. Methodology/Principal Findings Prior to...