A5077048577- Cancer Genomics and Diagnostics
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- HER2/EGFR in Cancer Research
- Bioinformatics and Genomic Networks
- Lymphoma Diagnosis and Treatment
- Orthopaedic implants and arthroplasty
- Pharmacological Receptor Mechanisms and Effects
- Adenosine and Purinergic Signaling
- Chronic Lymphocytic Leukemia Research
- Neuroinflammation and Neurodegeneration Mechanisms
- BRCA gene mutations in cancer
- AI in cancer detection
- Neuroscience and Neuropharmacology Research
- Chromosomal and Genetic Variations
- Cancer-related gene regulation
- Occupational and environmental lung diseases
- Multiple Sclerosis Research Studies
- Immunodeficiency and Autoimmune Disorders
- Bone Tissue Engineering Materials
- PARP inhibition in cancer therapy
- Diverticular Disease and Complications
- Breast Lesions and Carcinomas
- Pancreatitis Pathology and Treatment
- Tryptophan and brain disorders
Ospedale Policlinico San Martino
2024
Candiolo Cancer Institute
2021-2024
University of Turin
2021-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2021-2023
Azienda Ospedaliera Citta' della Salute e della Scienza di Torino
2020
University of Genoa
2014-2019
Dimethyl fumarate (DMF), recently approved as an oral immunomodulatory treatment for relapsing-remitting multiple sclerosis (MS), metabolizes to monomethyl (MMF) which crosses the blood-brain barrier and has demonstrated neuroprotective effects in experimental studies. We postulated that MMF exerts through modulation of microglia activation, a critical component neuroinflammatory cascade occurs neurodegenerative diseases such MS. To ascertain our hypothesis define mechanistic pathways...
Abstract Background The “HER2-low” nomenclature identifies breast carcinomas (BCs) displaying a HER2 score of 1+/2+ in immunohistochemistry and lacking ERBB2 amplification. Whether HER2-low BCs (HLBCs) constitute distinct entity is debated. Methods We performed DNA RNA high-throughput analysis on 99 HLBC samples ( n = 34 cases with 1+/HLBC-1, 15 2+ not amplified/HLBC-2N, 50 copy number the equivocal range/HLBC-2E). compared mutation rates data from 1317 Memorial Sloan-Kettering Cancer Center...
Immunogenic cell death (ICD) is a form of by which cancer treatments can induce clinically relevant antitumor immune response in broad range cancers. In multiple myeloma (MM), the proteasome inhibitor bortezomib an ICD inducer and creates durable therapeutic responses patients. However, eventual relapse resistance to appear inevitable. Here, integrating patient transcriptomic data with analysis calreticulin (CRT) protein interactors, we found that GABA type A receptor-associated (GABARAP)...
A new instrumentation exploiting magneto-dynamic technology (mallet) proposed for implant site preparation was investigated.In the tibias of three minipigs, two sites were prepared by mallet and drill technique. Primary stability (ISQ) detected after positioning (T0) at 14 days (T14). X-rays computed tomography performed. At T14, bone samples utilized histological biomolecular analyses.In sites, evaluations evidenced a significant increase in newly formed bone, osteoblast number, smaller...
Colorectal cancer (CRC) is a heterogeneous disease showing significant variability in clinical aggressiveness. Primary and acquired resistance limits the efficacy of available treatments, identification effective drug combinations needed to further improve patients’ outcomes. We previously found that NEDD8-activating enzyme inhibitor pevonedistat induced tumor stabilization preclinical models poorly differentiated, clinically aggressive CRC resistant therapies. To identify drugs can be...
Introduction: Optimization of pre-analytic procedures and tissue processing is a basic requirement for reliable reproducible data to be obtained. Tissue fixation in formalin represents the extensively favored method surgical specimen diagnostic pathology; however, exerts blasting effect on DNA RNA. Methods: A formic acid-deprived formaldehyde solution was prepared by removing acids with an ion-exchange resin concentrated, (ADF) employed prepare 4% ADF 0.1 M phosphate buffer, pH 7.2–7.4....
Background: Tubular carcinoma (TC) is a low proliferative grade 1 (G1) breast cancer (BC). Despite its favorable outcome and allegedly lower aggressiveness, patients are treated like other luminal G1 BC, with radiotherapy (RT) hormonal therapy (HT). We performed: (1) retrospective study comparing TC cohort control series of BC (2) systematic review meta-analysis focused on outcome. Materials Methods: selected 572 [111 TC, 350 not otherwise specified (NOS), 111 special-type (ST) BC] follow-up...
Formalin-fixed paraffin-embedded (FFPE) samples represent the cornerstone of tissue-based analysis in precision medicine. Targeted next-generation sequencing panels are routinely used to analyze a limited number genes guide treatment decision-making for advanced-stage patients. The and complexity genetic alterations be investigated rapidly growing; several instances, comprehensive genomic profiling is needed. poor quality material extracted from FFPE may impact feasibility/reliability data....
Agnostic biomarkers such as gene fusions allow to address cancer patients targeted therapies; however, the low prevalence of these alterations across common malignancies poses challenges and needs a feasible sensitive diagnostic process. RNA-based next generation sequencing was performed on 125 samples affected either by colorectal carcinoma, melanoma, or lung adenocarcinoma lacking genetic in canonical driver genes, carcinoma with microsatellite instability. Gene fusion rates were compared...
Two main variants of Richter syndrome (RS) are recognized, namely, the diffuse large B-cell lymphoma (DLBCL) and Hodgkin’s (HL) variant. Clonal relationship, defined as an identity immunoglobulin heavy chain variable (IGHV) region sequence between chronic lymphocytic leukemia (CLL) RS clones, characterizes patients with a poor prognosis. Due to method sensitivity, this categorization is performed without considering possibility small-size ancillary sharing same phenotype preexisting...
Abstract In multiple myeloma (MM), the proteasome inhibitor bortezomib (BTZ) triggers immunogenic cell death (ICD), a critical mechanism by which drug stimulates an anti-MM immune response. During ICD, dying cancer releases danger signals like calreticulin (CRT), attract dendritic cells (DCs) to engulf and present its antigens T cells. Cancer may employ several strategies restrain outcome, including interfering with exposure of CRT on surface. Such resistance mechanisms hinder activation...
Background: Long-Interspersed Nuclear Element (L1) retrotransposons are silenced in healthy tissues but unrepressed cancer. Even if L1 reactivation has been associated with reduced overall survival breast cancer (BC) patients, a comprehensive correlation clinicopathological features is still missing. Methods: Using quantitative, reverse-transcription PCR, we assessed mRNA expression 12 BC cells, 210 patients and 47 normal mammary tissues. was then correlated molecular data. Results: We...