A5086740780- Mosquito-borne diseases and control
- Viral Infections and Vectors
- CAR-T cell therapy research
- Virus-based gene therapy research
- Virology and Viral Diseases
- Hemophilia Treatment and Research
- HIV Research and Treatment
- Diabetes and associated disorders
- Chronic Myeloid Leukemia Treatments
- Immune Cell Function and Interaction
- Diet, Metabolism, and Disease
- Antibiotic Use and Resistance
- Antibiotic Resistance in Bacteria
- T-cell and B-cell Immunology
- Viral Infectious Diseases and Gene Expression in Insects
- Pancreatic function and diabetes
- Insect symbiosis and bacterial influences
- Genetic and phenotypic traits in livestock
- CRISPR and Genetic Engineering
- Immunodeficiency and Autoimmune Disorders
- Soybean genetics and cultivation
- Transgenic Plants and Applications
- Viral Infections and Outbreaks Research
- Plant Pathogens and Fungal Diseases
- Vector-borne infectious diseases
Indiana University School of Medicine
2019-2025
Indiana University – Purdue University Indianapolis
2019-2025
North Carolina Agricultural and Technical State University
2025
The Charutar Vidya Mandal (CVM) University
2024
Shree Guru Gobind Singh Tricentenary University
2021-2023
Indianapolis Zoo
2023
International Centre for Genetic Engineering and Biotechnology
2017-2019
Jaypee Institute of Information Technology
2011-2018
National Bureau of Plant Genetic Resources
2018
Gandhi Medical College & Hospital
2016
Regulatory T cells (Tregs) control immune responses in autoimmune disease, transplantation, and enable antigen-specific tolerance induction protein-replacement therapies. Tregs can exert a broad array of suppressive functions through their cell receptor (TCR) tissue-directed manner. This capacity now be harnessed for by "redirecting" polyclonal to overcome low inherent precursor frequencies simultaneously augment functions. With the use hemophilia A as model, we sought engineer suppress...
Hepatic adeno-associated viral (AAV) gene transfer has the potential to cure X-linked bleeding disorder hemophilia A. However, declining therapeutic coagulation factor VIII (FVIII) expression plagued clinical trials. To assess mechanistic underpinnings of this loss FVIII expression, we developed a A mouse model that shares key features observed in Following liver-directed AAV8 presence rapamycin, initial protein declines over time absence antibody formation. Surprisingly, production occurs...
Dengue virus (DENV), the causative agent of dengue disease, is among most important mosquito-borne pathogens worldwide. DENV composed four closely related serotypes and belongs to Flaviviridae family alongside other arthropod-borne viral such as Zika (ZIKV), West Nile (WNV) Yellow Fever (YFV). After infection, antibody response mostly directed E glycoprotein which three structural domains named DI, DII DIII that share variable degrees homology different viruses. Recent evidence supports a...
Abstract Dengue and Zika are two of the most important human viral pathogens worldwide. In both cases, envelope glycoprotein E is main target antibody response. Recently, new complex quaternary epitopes were identified which consequence arrangement antiparallel dimers on surface. Such can be exploited to develop more efficient cross-neutralizing vaccines. Here we describe a successful covalent stabilization from viruses in mammalian cells. Folding dimerization secretory was found strongly...
The flavivirus capsid protein (C) is separated from the downstream premembrane (PrM) by a hydrophobic sequence named anchor (Ca). During polyprotein processing, Ca sequentially cleaved viral NS2B/NS3 protease on cytosolic side and signal peptidase luminal of endoplasmic reticulum (ER). To date, considered important mostly for directing translocation PrM into ER lumen. In this study, role in assembly secretion Zika virus was investigated using pseudovirus-based approach. Our results show...
Adeno-associated virus (AAV) vectors are used for correcting multiple genetic disorders. Although the goal is to achieve lifelong correction with a single vector administration, ability redose would enable extension of therapy in cases which initial gene transfer insufficient lasting cure, episomal forms lost growing organs pediatric patients, or transgene expression diminished over time. However, AAV typically induces potent and long-lasting neutralizing antibodies (NAbs) against capsid...
Interpretation :The inter-relationships between the traits suggested that accessions with short flowering and maturity duration, low plant height, large bolls bold seeds should be given priority in breeding for enhanced yield.Donors various were identified which may used future linseed to target yield enhancement diverse geographical adaptation.
Limitations to successful gene therapy with adeno-associated virus (AAV) can comprise pre-existing neutralizing antibodies the vector capsid that block cellular entry, or inefficient transduction of target cells lead sub-optimal expression therapeutic transgene. Recombinant serotype 3 AAV (AAV3) is an emerging candidate for liver-directed therapy. In this study, we integrated rational design by using a combinatorial library derived from AAV3B capsids directed evolution in vitro selection...
The development of inhibitory antibodies (inhibitors) is a serious complication in the treatment hemophilia A with clotting factor VIII (FVIII) replacement therapy. Inhibitor formation critically depends on T cell help and modulation by regulatory cells (Tregs). In this study, we evaluated F5111 immunocytokine (IC), single chain fusion between human interleukin-2 (IL-2) cytokine an IL-2 antibody that biases activity towards high receptor alpha (IL-2Rα) expression, leading to extended...
Tyrosine protein-kinase 2 (TYK2) mediates inflammatory signalling through multiple cytokines, including interferon-α (IFNα), interleukin (IL)-12, and IL-23. TYK2 missense mutations protect against type 1 diabetes (T1D), inhibition of shows promise in other autoimmune conditions. We evaluated the effects specific inhibitors (TYK2is) pre-clinical models T1D, human β cells, cadaveric islets, iPSC-derived mouse models. In vitro studies showed that TYK2is prevented IFNα-induced cell HLA class I...
Formation of virus specific replicase complex is among the most important steps that determines fate viral transcription and replication during Chikungunya (CHIKV) infection. In present study, authors have computationally generated a 3D structure CHIKV late on basis interactions identified domains nonstructural proteins (nsPs) which make up complex. The nsPs were using systems such as pull down, protein interaction ELISA, yeast two-hybrid. structures I-TASSER biological assembly was...
Hemophilia A is an inherited coagulation disorder resulting in the loss of functional clotting factor VIII (FVIII). Presently, most effective treatment prophylactic protein replacement therapy. However, this requires frequent life-long intravenous infusions plasma derived or recombinant factors and not a cure. major complication development inhibitory antibodies that nullify factor. Immune tolerance induction (ITI) therapy to reverse inhibitors can last from months years, daily every other...
Tyrosine protein-kinase 2 (TYK2), a member of the Janus kinase family, mediates inflammatory signaling through multiple cytokines, including interferon-α (IFNα), interleukin (IL)-12, and IL-23. Missense mutations in TYK2 are associated with protection against type 1 diabetes (T1D), inhibition shows promise management other autoimmune conditions. Here, we evaluated effects specific inhibitors (TYK2is) pre-clinical models T1D. First, human β cells, cadaveric donor islets, iPSC-derived islets...
Chandipura virus (CHPV), alike other pathogens, exploits the cellular infrastructure of their hosts through complex network interactions for successful infection. CHPV being a recently emerged pediatric encephalitic virus, mechanisms involved in establishment viral persistence are still ill defined. Because protein interface between and its host provides one means by which invades seize control human machinery, authors this study have employed computational methods to create putative...