A5033259770- Extracellular vesicles in disease
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Neurobiology and Insect Physiology Research
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Organ Transplantation Techniques and Outcomes
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- RNA Interference and Gene Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Neurological disorders and treatments
- Tissue Engineering and Regenerative Medicine
- Heat shock proteins research
- Parkinson's Disease Mechanisms and Treatments
The University of Texas at Austin
2010-2023
Creative Commons
2023
Lone Star Paralysis Foundation
2023
Institute of Molecular and Cell Biology
2010
Exosome cargoes are highly varied and include proteins, small RNAs, genomic DNA (gDNA). The presence of gDNA suggests that different intracellular compartments contribute to exosome loading, resulting in distinct subpopulations. However, the loading other nuclear contents into exosomes (nExo) remains poorly understood. Here, we identify relationship between cancer cell micronuclei (MN), which markers instability, nExo formation. Imaging flow cytometry analyses reveal 10% derived from cells...
Parkinson's disease is associated with multiplication of the α-synuclein gene and abnormal accumulation protein. In animal models, overexpression broadly impairs synaptic vesicle trafficking. However, exact steps trafficking pathway affected by excess underlying molecular mechanisms remain unknown. Therefore we acutely increased synuclein levels at a vertebrate synapse performed detailed ultrastructural analysis effects on presynaptic membranes. At stimulated synapses (20 Hz), caused loss...
Despite the potential importance that synapse regeneration plays in restoring neuronal function after spinal cord injury (SCI), even most basic questions about morphology of regenerated synapses remain unanswered. Therefore, we set out to gain a better understanding central by examining number, distribution, molecular composition, and ultrastructure under conditions which behavioral recovery from SCI was robust. To do so, used giant reticulospinal (RS) neurons lamprey because they readily...
Neurotransmission requires a continuously available pool of synaptic vesicles (SVs) that can fuse with the plasma membrane and release their neurotransmitter contents upon stimulation. After fusion, SV membranes proteins are retrieved from presynaptic by clathrin-mediated endocytosis. internalization clathrin-coated vesicle, vesicle must uncoat to replenish SVs. Clathrin-coated uncoating ATP is mediated ubiquitous molecular chaperone Hsc70. In vitro , depolymerized clathrin forms stable...
Most organ or tissue allografts with viable cells are stored in solutions ex vivo for hours to several days. then require rapid host revascularization upon transplantation maintain donor-cell functions (e.g., cardiac muscle contractions, hepatic secretions). In contrast, peripheral nerve do not act as scaffolds guide outgrowth by axons at 1-2 mm/d, likely aided donor Schwann cells. Using current storage and protocols, all these organ/tissue/nerve transplants expected rapidly become...
Abstract Exosomes are small extracellular vesicles secreted from all living cells; its contents highly varied, including proteins, RNAs and recently discovered, genomic DNA (gDNA). The presence of nuclear content in exosomes suggests that different cell compartments can contribute to exosome cargo; however, little is known about this sub population derived (nExo). Here, we analyzed nExo isolated ovarian cancer cells characterized them explore potential clinical relevance such as detection...
Exosomes are small extracellular vesicles secreted from all living cells; its contents highly varied, including proteins, RNAs and recently discovered, genomic DNA (gDNA). The presence of nuclear content in exosomes suggests that different cell compartments can contribute to exosome cargo; however, little is known about this sub population derived (nExo). Here, we analyzed nExo isolated ovarian cancer cells characterized them explore potential clinical relevance such as detection therapeutic...