A5100403208- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Adipose Tissue and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Analytical chemistry methods development
- Signaling Pathways in Disease
- Diet and metabolism studies
- Crystal structures of chemical compounds
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Metal complexes synthesis and properties
- Microfluidic and Capillary Electrophoresis Applications
- RNA modifications and cancer
- Pesticide Residue Analysis and Safety
- Membrane Separation and Gas Transport
- Folate and B Vitamins Research
- Synthesis of Organic Compounds
- Graphene research and applications
- Metal-Organic Frameworks: Synthesis and Applications
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Metabolism and Genetic Disorders
- Natural product bioactivities and synthesis
- Advanced Proteomics Techniques and Applications
Institute of Physics
2025
Chinese Academy of Sciences
2016-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2008-2025
Harbin Medical University
2018-2025
First Affiliated Hospital of Harbin Medical University
2018-2025
The Synergetic Innovation Center for Advanced Materials
2025
Nanjing Tech University
2025
Ludwig Cancer Research
2024
Princeton University
2017-2024
Xi'an University of Technology
2022-2024
Accurate analysis of dinucleotide redox cofactors nicotinamide adenine phosphate reduced (NADPH), (NADP+), (NADH), and (NAD+) from biological samples is important to understanding cellular homeostasis. In this study, we aimed develop a simple protocol for quenching metabolism extracting NADPH that avoids interconversion among the forms oxidized forms.We compared seven different solvents extraction cultured mammalian cells mouse tissues: cold aqueous buffer commonly used in enzyme assays with...
One of the most intriguing questions in modern enzymology is whether enzyme dynamics evolved to enhance catalyzed chemical transformation. In this study, dihydrofolate reductase, a small monomeric protein that catalyzes single C-H-C transfer, used as model system address question. Experimental and computational studies have proposed dynamic network includes two residues remote from active site (G121 M42). The current study compares nature H-transfer step WT enzyme, mutants, their double...
Untargeted metabolomics can detect more than 10 000 peaks in a single LC–MS run. The correspondence between these and metabolites, however, remains unclear. Here, we introduce Peak Annotation Verification Engine (PAVE) for annotating untargeted microbial data. workflow involves growing cells 13C 15N isotope-labeled media to identify from biological compounds their carbon nitrogen atom counts. Improved deisotoping deadducting are enabled by algorithms that integrate positive mode, negative...
Fireworks displays are a traditional form of celebration during the Chinese Spring Festival (Festival). In response to heavy air pollution caused by fireworks, Shanghai, megacity in China, has imposed regulatory measures on use fireworks recent years. To investigate quality trends before and after firework regulation was established quantify its efficiency, gaseous pollutants, PM2.5 levels, chemical composition were synchronously measured at 1 h time intervals an urban site suburban Shanghai...
Relapsed/refractory acute myeloid leukemia (rrAML) is a malignant blood cancer with an extremely poor prognosis, largely ascribed to the drug-resistant stem cells (LSCs). Most patients suffer from risk of difficult-to-cure as well severe systemic toxicity when receiving standard chemotherapies. As hyaluronic acid (HA) specific ligand CD44 highly expressed by LSCs, we had HA self-assembled cisplatin and daunorubicin form dual chemodrug nanogel (HA/Cis/Dau) afford targeted therapeutic...
Subcellular localization directed by specific targeting motifs is an emerging theme for regulating signal transduction pathways. For cAMP-dependent protein kinase (PKA), this achieved primarily its association with A-kinase–anchoring proteins (AKAPs). Dual specificity AKAP1, (D-AKAP1) binds to both type I and II regulatory subunits has two NH2-terminal (N0 N1) COOH-terminal (C1 C2) splice variants (Huang et al., 1997. J. Biol. Chem. 272:8057). Here we report that the of D-AKAP1 are expressed...
Non-contact, continuous enrichment of low-abundance cell samples in microfluidics is demonstrated<italic>via</italic>the standing surface acoustic waves (SSAW).
China has high stroke rates despite the population being relatively lean. Uncertainty persists about relevance of adiposity to risk types. We aimed assess associations with incidence types and effect mediation by blood pressure in Chinese men women.The Kadoorie Biobank enrolled 512 891 adults aged 30-79 years from ten areas (five urban five rural) during 2004-08. During a median 9 (IQR 8-10) follow-up, 32 448 strokes (about 90% confirmed neuroimaging) were recorded among 489 301 participants...
To evaluate the effects of chelated Zn/Cu/Mn on redox status, immune responses and hoof health in lactating Holstein cows, 48 head early lactation were divided into healthy or lame groups according to their gait score.Cows fed same amount as sulfate salts forms for 180 days, foot-and-mouth disease (FMD) vaccine was injected at day 90.The results showed that cows had lower antioxidant function, serum Zn/Mn levels, hair Cu hardness.Moreover, increased FMD antibody titers, levels Zn/Cu/Mn,...
Dried blood spots (DBS) have proven to be a powerful sampling and storage method for newborn screening many other applications. However, DBS methods not yet been optimized broad-spectrum targeted metabolomic analysis. In this study, we developed robust, DBS-based, broad-spectrum, that was able measure over 400 metabolites from 6.3 mm punch standard Whatman 903TM filter paper cards. The effects of spot volumes, hematocrit, vacutainer chemistry, extraction methods, carryover, comparability...
Hybrid membranes of metal–organic molecule nanocages were fabricated on a ceramic hollow tube by simple immerse-coating method, which exhibit excellent pervaporation separation performances towards the aromatic–aliphatic hydrocarbon mixture.