A5100724543- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Sensor Technologies
- Birth, Development, and Health
- Liver Disease Diagnosis and Treatment
- Fermentation and Sensory Analysis
- Mass Spectrometry Techniques and Applications
- Horticultural and Viticultural Research
- Nutritional Studies and Diet
- Diet and metabolism studies
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Renal cell carcinoma treatment
- Metabolism and Genetic Disorders
- Peripheral Neuropathies and Disorders
- Gestational Diabetes Research and Management
- Bladder and Urothelial Cancer Treatments
- Analytical Chemistry and Chromatography
- Computational Drug Discovery Methods
- Pregnancy and preeclampsia studies
- Neonatal and fetal brain pathology
- Infectious Encephalopathies and Encephalitis
- Environmental DNA in Biodiversity Studies
- Nutrition and Health in Aging
- Cytomegalovirus and herpesvirus research
- Pancreatitis Pathology and Treatment
Universidade do Porto
2017-2025
University of Aveiro
2010-2025
Research Center Pharmaceutical Engineering (Austria)
2025
Centro de Estudos Ambientais e Marinhos
2025
Rede de Química e Tecnologia
2017-2024
Hospital Braga
2021-2024
Unidade em Ciências Biomoleculares Aplicadas
2024
Universidade Nova de Lisboa
2024
Campo Arqueologico de Mertola
2024
European Bioinformatics Institute
2024
Aging promotes oxidative stress in vascular endothelial and smooth muscle cells, which contribute to the development of cardiovascular diseases. NF-E2–related factor 2 (Nrf2) is a transcription factor, activated by reactive oxygen species vasculature young animals, leading adaptive upregulation numerous detoxifying antioxidant genes. The present study was designed elucidate age-associated changes homeostatic role Nrf2-driven free radical detoxification mechanisms nonhuman primates. We found...
The stability of human plasma composition was investigated by NMR, considering different collection tubes, time at room temperature (RT), short- and long-term storage conditions up to 5 consecutive freeze–thaw cycles.
This work describes an exploratory NMR metabonomic study of second trimester maternal urine and plasma, in attempt to characterize the metabolic changes underlying prenatal disorders identify possible early biomarkers. Fetal malformations have strongest impact both biofluids, suggesting effects due hypoxia (leading hypoxanthine increased excretion) a need for enhanced gluconeogenesis, with higher ketone bodies (acetone 3-hydroxybutyric acid) production TCA cycle demand (suggested by...
The lack of sensitive and specific biomarkers for the early detection prostate cancer (PCa) is a major hurdle to improve patient management.A metabolomics approach based on GC-MS was used investigate performance volatile organic compounds (VOCs) in general and, more specifically, carbonyl (VCCs) present urine as potential markers PCa detection.Results showed that patients (n = 40) can be differentiated from cancer-free subjects 42) their urinary profile both VOCs VCCs models, unveiling...
This paper describes a metabonomic study of prenatal disorders using nuclear magnetic resonance (NMR) spectroscopy amniotic fluid (AF) collected in the second trimester pregnancy, to search for metabolite markers fetal malformations, prediagnostic gestational diabetes (GD), preterm delivery (PTD), early rupture membranes (PROM), and chromossomopathies. Fetal malformations were found have highest impact on AF composition, enabling statistical validation be achieved by several multivariate...
This work presents the first NMR metabolomics study of maternal plasma during pregnancy, including correlation between and urine metabolites. The expected decrease in circulating amino acids early pregnancy was confirmed with six being identified as required by fetus larger extents. Newly observed changes citrate, lactate, dimethyl sulfone suggested adjustments energy gut microflora metabolisms. Alterations creatine levels were also noted, addition to creatinine variations reflecting...
Metabolic biomarkers of pre- and postdiagnosis gestational diabetes mellitus (GDM) were sought, using nuclear magnetic resonance (NMR) metabolomics maternal plasma corresponding lipid extracts. Metabolite differences between controls disease identified through multivariate analysis variable selected (1)H NMR spectra. For GDM, partial least squares regression metabolites with higher dependence on normal age evolution. Variable selection spectra produced good classification models for both...
The analysis of volatile organic compounds (VOCs) emanating from biological samples appears as one the most promising approaches in metabolomics for study diseases, namely cancer. In fact, it offers advantages, such non-invasiveness and robustness high-throughput applications. purpose this work was to urinary metabolic profile patients with renal cell carcinoma (RCC) (n = 30) controls 37) aim identifying a potential specific pattern non-invasive strategy detect RCC. Moreover, effect some...
Serum prostate-specific antigen (PSA) is currently the most used biomarker in clinical practice for prostate cancer (PCa) detection. However, this has several drawbacks. In work, an untargeted gas chromatography-mass spectrometry (GC-MS)-based metabolomic profiling of PCa cells was performed to prove concept that metabolic alterations might differentiate cell lines from normal line. For that, we assessed differences volatile organic compounds (VOCs) profile extracellular medium...
Timely diagnosis is crucial to improve the long-term survival of bladder cancer (BC) patients. The discovery new BC biomarkers based in urine analysis very attractive because this biofluid direct contact with inner layer, which most neoplasms develop, and non-invasively collected. Hence, work aimed unveil alterations urinary volatile profile patients diagnosed compared cancer-free individuals, as well differences among at different tumor stages, identify candidate for non-invasive staging....
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease in which the death of motor neurons leads to loss muscle function. Additionally, cognitive and circadian disruptions are common ALS patients, contributing progression burden. Most cases sporadic, environmental exposures contribute their aetiology. However, animal models these sporadic scarce. The small vertebrate zebrafish leading organism model diseases; previous studies have proposed bisphenol A (BPA) or...
Agriculture is vital for supporting human populations, but its intensification often leads to landscape homogenization and a decline in non-provisioning ecosystem services. Ecological multifunctional landscapes are suggested as nature-based alternatives intensive agriculture, using ecological processes like natural pest regulation maximize food production. Birds recognized their role increasing crop yields by consuming invertebrate pests several agroecosystems. However, the understanding of...
Abstract RCC usually develops and progresses asymptomatically and, when detected, it is frequently at advanced stages metastatic, entailing a dismal prognosis. Therefore, there an obvious demand for new strategies enabling earlier diagnosis. The importance of metabolic rearrangements carcinogenesis unlocked approach cancer research, catalyzing the increased use metabolomics. present study aimed NMR profiling in urine samples from cohort patients ( n = 42) controls 49). methodology entailed...
Clear cell renal carcinoma (ccRCC) is the most common type of kidney cancer usually associated with asymptomatic development and risk systemic progression. Hence, reliable molecular biomarkers ccRCC are needed to provide early minimally invasive detection. In this study, urinary volatilome profiling patients diagnosed (n = 75), cancer-free controls was performed investigate presence a volatile signature characteristic ccRCC. Volatile organic compounds (VOCs) in general, more specifically...
In humans, growth hormone deficiency (GHD) and low circulating levels of insulin-like factor 1 (IGF-1) significantly increase the risk for cerebrovascular disease. Genetic (GH)/IGF-1 in Lewis dwarf rats increases incidence late-life strokes, similar to effects GHD elderly humans. Peripubertal treatment with GH delays occurrence stroke, which results a significant extension life span. The present study was designed characterize vascular span-extending peripubertal replacement rats. Here, we...