A5035312583- Collagen: Extraction and Characterization
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Environmental Impact and Sustainability
- Enzyme Catalysis and Immobilization
- Silk-based biomaterials and applications
- Sustainable Supply Chain Management
- Fluid Dynamics and Mixing
- Innovative Microfluidic and Catalytic Techniques Innovation
- Dermatologic Treatments and Research
- Bone and Dental Protein Studies
- Energy Efficiency and Management
- Meat and Animal Product Quality
- Contact Dermatitis and Allergies
- Catalysis for Biomass Conversion
- Connective tissue disorders research
- Dental materials and restorations
- Antioxidant Activity and Oxidative Stress
- Urban and Freight Transport Logistics
- Osteoarthritis Treatment and Mechanisms
- Periodontal Regeneration and Treatments
- Environmental Sustainability in Business
- Circadian rhythm and melatonin
- Anaerobic Digestion and Biogas Production
- Polysaccharides Composition and Applications
Cranfield University
2021-2024
Institute of Animal Physiology of the Slovak Academy of Sciences
1965-1980
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria
1965-1978
University of Dundee
1976
Auckland City Hospital
1954
SYNOPSIS Hypotheses to explain the formation of native‐collagen fibrils from tropocollagen macromolecules have been considered and their inconsistencies examined. A new model for macromolecule is suggested a mechanism its assembly into offered. It postulated that divided 5 main bonding zones separated by 4 non‐bonding zones. The are assembled native allowing zone on particular an initial random choice in combining, structurally complementary manner, with another macromolecule. evidence...
Acetate is emerging as a promising feedstock for biorefineries it can serve an alternate carbon source microbial cell factories. In this study, we expressed acetyl-CoA synthase in Yarrowia lipolytica PSA02004PP, and the recombinant strain grew on acetate sole accumulated succinic acid or succinate (SA). Unlike traditional feedstocks, toxic substrate microorganisms; therefore, was further subjected to adaptive laboratory evolution alleviate toxicity improve tolerance against acetate. At high...
Lactic acid (LA) is a platform chemical with diverse industrial applications. Presently, commercial production of LA dominated by microbial fermentation using sugary or starch-based feedstocks. Research pursuits emphasizing towards sustainable non-edible and renewable feedstocks have accelerated the use lignocellulosic biomass (LCB). The present study focuses on valorisation xylose derived from sugarcane bagasse (SCB) olive pits (OP) through hydrothermal dilute pretreatment, respectively....
SYNOPSIS An electron‐microscope study has been made of collagen fibrils before and after treatment with various cross‐linking reagents. In general it was found that resulted in an increase the size light‐staining bands (as visualized by negative staining) concluded light native correspond to regions where tropocollagen macromolecules are bonded together. Treatment fibrous‐long‐spacing form long period 2300 Å being converted a native‐type 640 periodicity.
The possibility of using allograft collagen for the permanent replacement lost or damaged connective tissues has been examined in rat. cellular components skin, which are known to be major importance rejection, were removed by treating skin with a solution crystalline trypsin at 15 degrees C. Non-collagenous structures largely 7 days, but purification process continued up 28 days without damage fibrils. Dermal allografts, implanted intraperitoneally subcutaneously and biopsied 3-83 after...
ABSTRACT Native and cross-linked rat tail tendon collagen was irradiated with high energy electrons up to a maximum dose of 100 × 104 J kg − 1 (100 Mrd). Fibres dry showed greater damage when examined in the electron microscope using negative staining techniques than those wet. Cross-linking glutaraldehyde prior irradiation resulted band structure being preserved even at highest but an unequal shrinkage bands noted. Irradiation altered reactivity both native collagenase elastase. Wet-but not...
ABSTRACT Rat tail tendon collagen has been treated with the monoaldehydes, formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde and benzaldehyde, dialdehydes, glyoxal glutaraldehyde. The was then examined in electron microscope using a negative stain. Alterations to native pattern were seen A correlation noted between changes band degree of cross-linking estimated from chemical physical measurements made by other workers. results indicate that use microscopy staining may be valuable...
ABSTRACT Deamination of native rat tail collagen followed by treatment with 1,2-cyclohexanedione removes the positive charge from side chains lysine and hydroxylysine residues blocks approximately 70 % positively charged arginine residues. When such specimens are examined in electron microscope after negative staining 2 potassium phosphotungstate at pH 6 ·8 –7 ·4 no change appearance normal is detectable. These findings suggest that electrostatic attraction does not occur between stain...
SUMMARY Glutaraldehyde‐crosslinked rat‐tail collagen, compared with native fibres, showed an increase in the size of light bands when examined electron microscope using a negative‐staining technique. Collagen deaminated nitrous acid gave similar patterns to fibres and no change after treatment glutaraldehyde. It was concluded that is due formation intermolecular crosslinks rather than charge neutralization lysine amino groups.
Quantification of liquid-phase plug flow within an oscillatory baffle reactor using the tanks-in-series model.
An oscillatory baffled flow reactor (OBR) has been designed with 60 interbaffled cells. The columns of 40 mm internal diameter together result in a length 5740 mm. amplitude and frequency were the range 2-12 0.3-2 Hz, respectively. report investigates impact U-bends number sections on axial dispersion for scale-up feasibility. A prediction model using operating parameters developed to maximize plug conditions tanks-in-series (TiS) model. maximum TiS value was 13.38 single column compared...