A5063636724- Estrogen and related hormone effects
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Advanced Breast Cancer Therapies
- HER2/EGFR in Cancer Research
- Breast Cancer Treatment Studies
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Melanoma and MAPK Pathways
- RNA modifications and cancer
- Circular RNAs in diseases
- Molecular Biology Techniques and Applications
- Cancer Treatment and Pharmacology
- Mast cells and histamine
- Multiple Myeloma Research and Treatments
- Retinoids in leukemia and cellular processes
- Renal cell carcinoma treatment
- Protein Degradation and Inhibitors
- Immune cells in cancer
- Cancer, Lipids, and Metabolism
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2022-2025
Vita-Salute San Raffaele University
2022-2025
Fondazione IRCCS Istituto Nazionale dei Tumori
2015-2025
IRCCS Ospedale San Raffaele
2020-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2022-2025
San Raffaele University of Rome
2022
Tumori Foundation
2022
University of Milan
2015
Fudan University Shanghai Cancer Center
2013
Garvan Institute of Medical Research
2012
It is established that the interaction between microenvironment and cancer cells has a critical role in tumor development, given dependence of neoplastic on stromal support. However, how this communication promotes activation normal (NFs) into cancer-associated fibroblasts (CAFs) still not well understood. Most microRNA (miRNA) studies focused cell, but there increasing evidence their involvement reprogramming NFs CAFs. Here we show miR-9, upregulated various breast cell lines identified as...
The accrual of myeloid-derived suppressor cells (MDSCs) represents a major obstacle to effective immunotherapy in cancer patients, but the mechanisms underlying this process human setting remain elusive. Here, we describe set microRNAs (miR-146a, miR-155, miR-125b, miR-100, let-7e, miR-125a, miR-146b, miR-99b) that are associated with MDSCs and resistance treatment immune checkpoint inhibitors melanoma patients. miRs were identified by transcriptional analyses as being responsible for...
Abstract Immune checkpoint blockade (ICB) benefits some patients with triple-negative breast cancer, but what distinguishes responders from non-responders is unclear 1 . Because ICB targets cell–cell interactions 2 , we investigated the impact of multicellular spatial organization on response, and explored how remodels tumour microenvironment. We show that cell phenotype, activation state location are intimately linked, influence effect differ in sensitive versus resistant tumours early...
Kidney donation after circulatory death (DCD) is a less than ideal option to meet organ shortages. Hypothermic machine perfusion (HMP) with Belzer solution (BS) improves the viability of DCD kidneys, although graft clinical course remains critical. Mesenchymal stromal cells (MSC) promote tissue repair by releasing extracellular vesicles (EV). We evaluated whether delivering MSC-/MSC-derived EV during HMP protects rat kidneys from ischaemic injury and investigated underlying pathogenic...
// Emanuela Minna 1,* , Paola Romeo Loris De Cecco 2 Matteo Dugo Giuliana Cassinelli 3 Silvana Pilotti 4 Debora Degl'Innocenti 1 Cinzia Lanzi Patrizia Casalini 5 Marco A. Pierotti 6 Angela Greco and Maria Grazia Borrello Molecular Mechanisms Unit, Department of Experimental Oncology Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy Functional Genomics Core Facility, Pharmacology Pathology, Targeting Scientific Directorate, * These authors contributed equally to the work...
// Elisabetta Vergani 1, * , Lorenza Di Guardo 2, Matteo Dugo 3, Sara Rigoletto 1 Gabrina Tragni 4 Roberta Ruggeri 5 Federica Perrone Elena Tamborini Annunziata Gloghini Flavio Arienti 6 Barbara 7 Paola Deho Loris De Cecco 3 Viviana Vallacchi Frati Eriomina Shahaj Antonello Villa Mario Santinami Filippo Braud 2 Licia Rivoltini Monica Rodolfo Immunotherapy Unit, Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy Medical...
Abstract In multiple myeloma, novel treatments with proteasome inhibitors (PIs) and immunomodulatory agents (IMiDs) have prolonged survival but the disease remains incurable. At relapse, next-generation sequencing has shown occasional mutations of drug targets failed to identify unifying features that underlie chemotherapy resistance. We studied 42 patients refractory both PIs IMiDs. Whole-exome was performed in 40 patients, RNA (RNA-seq) 27. found more than were reported at diagnosis...
This study aimed to identify circulating miRNAs as novel non-invasive biomarkers for prognosis and vandetanib response in advanced medullary thyroid cancer (MTC) patients. We prospectively recruited two independent cohorts of locally advanced/metastatic MTC patients including a subgroup vandetanib-treated subjects: discovery cohort (n = 20), matched plasma/tissue samples 17/20), validation cohort, yielding only plasma 17). Plasma from healthy subjects 36) remission 9) were used controls. 17...
Progression to stage IV disease remains the main cause of breast cancer-related deaths. Increasing knowledge on hematogenous phase metastasis is key for exploiting entire window opportunity interfere with early dissemination and achieve a more effective control. Recent evidence suggests that circulating tumor cells (CTCs) possess diverse adaptive mechanisms survive in blood eventually metastasize, encouraging research into CTC-directed therapies.On hypothesis distinguishing molecular...
We assessed the 27-gene RT-qPCR-based DetermaIO assay and same score calculated from RNA sequencing (RNA-seq) data as predictors of sensitivity to immune checkpoint therapy in neoTRIPaPDL1 randomized trial that compared neoadjuvant carboplatin/nab-paclitaxel chemotherapy (CT) plus atezolizumab with CT alone stage II/III triple-negative breast cancer. also predictive function immuno-oncology (IO) expression patients treated pembrolizumab paclitaxel (N = 29) or 56) I-SPY2 trial.
Though miR-205 function has been largely characterized, the nature of its host gene, MIR205HG, is still completely unknown. Here, we show that only lowly expressed alternatively spliced MIR205HG transcripts act as de facto pri-miRNAs, through a process involves Drosha to prevent unfavorable splicing and directly mediate excision. Notably, MIR205HG-specific processed revealed be functional per se nuclear long noncoding RNA capable regulating differentiation human prostate basal cells control...
Abstract Tumor growth and development is determined by both cancer cell–autonomous microenvironmental mechanisms, including the contribution of infiltrating immune cells. Because role mast cells (MC) in this process poorly characterized even controversial, we investigated their part breast cancer. Crossing C57BL/6 MMTV-PyMT mice, which spontaneously develop mammary carcinomas, with MC-deficient C57BL/6-KitW-sh/W-sh (Wsh) showed that MCs promote tumor prevent basal CK5-positive areas favor a...
Thyroid carcinoma (TC) comprises several histotypes with different aggressiveness, from well (papillary carcinoma, PTC) to less differentiated forms (poorly and anaplastic thyroid PDTC ATC, respectively). Previous reports have suggested a functional role for cancer-associated fibroblasts (CAFs) or senescent TC cells in the progression of PTC. In this study, we investigated presence CAFs proprietary human TCs including PTC, PDTC, ATC. Screening driving lesions BRAFV600E N/H/KRAS mutations,...
Antibody-drug conjugates (ADCs) are a rapidly expanding class of compounds in oncology. Our goal was to assess the expression ADC targets and potential downstream determining factors activity across pan-cancer normal tissues.
Background Microarray technology applied to microRNA (miRNA) profiling is a promising tool in many research fields; nevertheless, independent studies characterizing the same pathology have often reported poorly overlapping results. miRNA analysis methods only recently been systematically compared but few cases using clinical samples. Methodology/Principal Findings We investigated inter-platform reproducibility of four microarray platforms (Agilent, Exiqon, Illumina, and Miltenyi), comparing...
We have previously shown that the d16HER2 splice variant is linked to HER2-positive breast cancer (BC) tumorigenesis, progression and response Trastuzumab. However, mechanisms by which contributes HER2-driven aggressiveness targeted therapy susceptibility remain uncertain. Here, we report d16HER2-positive mammary tumor cell lines MI6 MI7, derived from spontaneous lesions of transgenic (tg) mice resembling aggressive features primary lesions, are enriched in expression Wnt, Notch...
// Matteo Dugo 1 , Gabriella Nicolini 2 Gabrina Tragni 3 Ilaria Bersani Antonella Tomassetti 4 Valentina Colonna 5 Michele Del Vecchio Filippo De Braud Silvana Canevari Andrea Anichini 2, * Marialuisa Sensi 1, Functional Genomics and Bioinformatics, Department of Experimental Oncology Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy Unit Immunobiology Human Tumors, Pathology, Therapies, Clinical Oncology, These authors have contributed equally to this work...
Breast cancer clinical outcome is affected by tumor molecular features, and the identification of subtype-specific prognostic biomarkers relevant for breast translational research. Gene expression signatures proved to be able complement information provided classical clinico-pathological features. Recently, microRNAs (miRNAs) have been causally linked tumorigenesis progression associated with patient outcome, also in cancer. MicroRNAs development distant metastasis were identified a cohort...
Abstract Background Papillary thyroid cancer (PTC) is the most frequent endocrine tumor. Radioiodine (RAI) treatment highly effective in these tumors, but up to 60% of metastatic cases become RAI-refractory. Scanty data are available on either molecular pattern radioiodine refractory papillary cancers or mechanisms responsible for RAI resistance. Methods We analyzed profile and gene/miRNA expression primary PTCs, synchronous RAI-refractory lymph node metastases (LNMs) correlation avidity...