A5048623304- Syphilis Diagnosis and Treatment
- Reproductive tract infections research
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Leptospirosis research and findings
- HIV/AIDS oral health manifestations
- Sex work and related issues
- Virology and Viral Diseases
- Rabies epidemiology and control
- Genital Health and Disease
- HIV/AIDS Research and Interventions
- Medicine and Dermatology Studies History
- HIV, TB, and STIs Epidemiology
- Cuban History and Society
- Immunodeficiency and Autoimmune Disorders
Instituto de Medicina Tropical “Pedro Kourí”
2013-2023
Ministerio de Salud Pública
2016
University of Havana
2014
Syphilis, caused by Treponema pallidum subsp. (TPA), remains an important public health problem with increasing worldwide prevalence. Despite recent advances in vitro cultivation, genetic variability of this pathogen during infection is poorly understood. Here, we present contemporary and geographically diverse complete treponemal genome sequences isolated directly from patients using a methyl-directed enrichment prior to sequencing. This approach reveals that approximately 50% the diversity...
Four variants of the potassium acetate procedure for DNA extraction from ixodid ticks at different stage their life cycles were evaluated and compared with phenol-chloroform ammonium hydroxide methods. The most rapid efficient variant was validated in engorged collected bovine, canine as well house screening Borrelia burgdorferi sensu lato, Anaplasma spp. Babesia used non-engorged ticks. All allowed obtaining PCR-quality material according to specific amplification 16S rRNA gene fragment...
The increased prevalence of syphilis in Cuba prompted us to map the circulating Treponema pallidum subsp. allelic profiles this geographic region. Samples were collected from 2012 2017, 83 male patients with ulcers or skin lesions, and examined using multilocus sequence typing. Additionally, we analyzed 23S rDNA 16S regions for presence possible mutations leading macrolide tetracycline resistance. Among 94% fully typed strains, found 7 different profiles, which 4 had not been previously...
To describe and analyze the clinical epidemiological status in 28 confirmed cases of human leptospirosis at main public hospital Cordoba.Between 2012 2013, we conducted an active surveillance Cordoba to establish etiologic diagnosis undifferentiated tropical febrile illness (UTFI) cases. UTFI is defined as a fever without infection focus initial physical examination or basic laboratory tests. Patients acute phase were accompanied by prodromal symptoms, including myalgia, arthralgia,...
Syphilis, caused by the spirochete Treponema pallidum subspecies pallidum, is a rising global public health concern and laboratory diagnostics remain challenging. Especially during early disease, rapid accurate diagnosis crucial to ensure patients their contacts receive timely treatment eradicate infection prevent further transmission. In this prospective observational study, we evaluated performance of polymerase chain reaction (PCR) serological testing for primary syphilis evaluating...
1. Heyman P, Cochez C, Hofhuis A, van der Giessen J, Sprong H, Porter SR, Losson B, Saegerman Donoso-Mantke O, Niedrig M, Papa A. A clear and present danger: tick-borne diseases in Europe. Expert Rev Anti Infect Ther. 2010; 8(1): 33–50. CrossRef Google Scholar
This study aimed to assess the presence of macrolide-resistant Treponema pallidum subtypes in Havana, Cuba. Samples from 41 syphilis patients were tested for T. 23S rRNA gene mutations. Twenty-five (61%) harbored with A2058G mutation, which was present all 8 that identified. The A2059G mutation not detected.
This study describes the development and application of a new PCR assay for specific detection pathogenic leptospires its comparison with previously reported protocol. New primers were designed optimization evaluation in artificially-infected paraffin-embedded tissues. was then applied to post-mortem, samples, followed by amplicon sequencing. The more efficient than protocol, allowing amplification expected DNA fragment from artificially infected samples 44% post-mortem samples. sequences...
Leptospirosis is a neglected disease causing severe infections in humans and animals. Due part to misdiagnosis, this infectious results nearly 60,000 deaths per year around the globe. This study represents first effort describe diversity of pathogenic Leptospira Cuba based on whole-genome sequencing. We have collected nineteen whole-blood samples from patients that were diagnosed as having leptospirosis between 2008 2012 Cuba. In addition, we enhanced our sample set by three historical...
Sexually transmitted diseases (STDs) and in particular genital ulcer disease (GUD) have a major impact on morbidity mortality developing countries. The World Health Organization recommends the use of syndromic guidelines for treatment sexually infections (STIs) resource-constrained Surveillance autochthonous etiologies provides epidemiological information contributing to prevention STIs. We investigated etiology factors associated with GUD among male patients attending STD clinic Havana,...
Bejel (endemic syphilis) is a neglected non-venereal disease caused by Treponema pallidum subsp. endemicum (TEN). Although it mostly present in hot, dry climates, few cases have been found outside of these areas. The aim this work was the sequencing and analysis TEN isolates obtained from "syphilis patients" Cuba, which not considered an endemic area for bejel. Genomes were pool segment genome or direct methods, bioinformatics performed according to established pipeline. We four genomes with...
Congenital syphilis is a potentially devastating infection that results from mother to child transmission (MTCT) of Treponema pallidum subspecies (T. pallidum), the etiological agent syphilis, via placenta during pregnancy.1 Mother can be prevented by universal screening for maternal early in pregnancy, preferably first trimester, and treatment infected women their sexual partners with intramuscular benzathine penicillin G (BPG), recommended first-line drug syphilis.1,2 In 2007, World Health...
"The Cuban Experience in the Elimination of Mother-to-Child Transmission Congenital Syphilis." , 106(11), pp. 1975–1976
Objectives This study aimed to determine the allelic profiles of Treponema pallidum in patients confirmed with syphilis Cuba (2018–2019) and explore mutations leading macrolide tetracycline resistance. Methods Multilocus sequence typing polymerase chain reaction rrn loci (23S 16S rDNA), followed by Sanger sequencing, were used define profile TPA resistance mutations, respectively. Results Allelic 1.3.1 recombinant identified, 15.7.3 having an increased frequency. We did not detect presence...
Serosurveys for IgG antibodies to Borrelia burgdorferi sensu stricto in a population exposed tick bites (n = 247) and blood donors 114) were done assess the prevalence of tick-borne infections in. Seroprevalence antiborrelial was estimated 0.6–7.2 % 0 risk donors, respectively. While previous expositions A. phagocytophilum (7.2 %), E. chaffensis (3.6 %) B. microti (11.5 serologically detected. These reports suggest presence pathogens Cuba, nonetheless lacking further accurate information...
Four variants of the potassium acetate procedure for DNA extraction from ixodid ticks at different stage their life cycles were evaluated and compared with phenol-chloroform ammonium hydroxide methods. The most rapid efficient variant was validated in engorged collected bovine, canine as well house screening Borrelia burgdorferi sensu lato, Anaplasma spp. Babesia used non-engorged ticks. All allowed obtaining PCR-quality material according to specific amplification 16S rRNA gene fragment...
Abstract Bejel (endemic syphilis) is a neglected non-venereal disease caused by Treponema pallidum subsp. endemicum (TEN). Although it mostly present in hot, dry climates, few cases have been found outside of these areas. The aim this work was the sequencing and analysis TEN isolates obtained from “syphilis patients” Cuba, which not considered an endemic area for bejel. Genomes were pool segment genome or direct methods, bioinformatics performed according to established pipeline. We four...