A5051942598- Syphilis Diagnosis and Treatment
- Reproductive tract infections research
- Sex work and related issues
- Leptospirosis research and findings
- Probiotics and Fermented Foods
- Virology and Viral Diseases
- HIV/AIDS oral health manifestations
- Veterinary medicine and infectious diseases
- Mycobacterium research and diagnosis
- HIV, TB, and STIs Epidemiology
- Immunodeficiency and Autoimmune Disorders
- Enterobacteriaceae and Cronobacter Research
- Bacteriophages and microbial interactions
- Gut microbiota and health
- RNA and protein synthesis mechanisms
- Botany and Plant Ecology Studies
- Microbial Community Ecology and Physiology
- Zoonotic diseases and public health
- Infectious Diseases and Mycology
- Antimicrobial Peptides and Activities
- Microbial Metabolites in Food Biotechnology
- Genetic factors in colorectal cancer
- Autoimmune Bullous Skin Diseases
- Food Industry and Aquatic Biology
- Herbal Medicine Research Studies
Masaryk University
2015-2024
Wellcome Sanger Institute
2022-2024
Institut Pasteur
2019-2023
Brno University of Technology
2023
Université Paris Cité
2022-2023
Centre National de la Recherche Scientifique
2023
Ministry of Health
2014-2019
Instituut voor Tropische Geneeskunde
2017
Syphilis is an important public health problem and increasing incidence has been noted in recent years. Characterization of strain diversity through molecular data plays a critical role the epidemiological understanding this re-emergence. We here propose new high-resolution multilocus sequence typing (MLST) scheme for Treponema pallidum subsp. (TPA). analyzed 30 complete draft TPA genomes obtained directly from clinical samples or rabbit propagated strains to identify suitable loci tested on...
ABSTRACT From January 2011 to December 2013, a total of 262 samples, from 188 patients suspected having syphilis were tested for the presence treponemal DNA by PCR amplification five chromosomal loci, including polA (TP0105), tmpC (TP0319), TP0136, TP0548, and 23S rRNA genes. Altogether, 146 samples 103 positive DNA. A set 81 62 PCR-positive typeable, among them, nine different genotypes identified. Compared previous study in Czech Republic during 2004 2010, number detected particular year...
Syphilis, caused by Treponema pallidum subsp. (TPA), remains an important public health problem with increasing worldwide prevalence. Despite recent advances in vitro cultivation, genetic variability of this pathogen during infection is poorly understood. Here, we present contemporary and geographically diverse complete treponemal genome sequences isolated directly from patients using a methyl-directed enrichment prior to sequencing. This approach reveals that approximately 50% the diversity...
Treponema pallidum ssp. (TPA) causes over 10 million new cases of syphilis worldwide whereas T. pertenue (TPE), the causative agent yaws, affects about 2.5 people. Although penicillin remains drug choice in treatment syphilis, penicillin-allergic patients, macrolides have been used this indication since 1950s. Failures well documented literature and 2000, there has a dramatic increase number clinical samples with macrolide-resistant TPA. Scarce data regarding genetics mutations TPA suggest...
A total of 54 clinical samples, including genital lesion swabs, whole blood and cerebrospinal fluid from patients diagnosed with syphilis were collected in 2006 2013 Buenos Aires, Argentina. Treponemal DNA was detected 43 the analyzed samples (79.6%) further using Sequencing-based molecular typing (SBMT) Enhanced CDC-typing (ECDCT). By SBMT, 10 different Treponema pallidum subsp. (TPA) genotypes found, which six related to TPA SS14 strain, four Nichols strain. The 23S rRNA gene amplified...
Treponema pallidum subsp. pallidum, the causative agent of sexually transmitted syphilis, detected in clinical samples from France, was subjected to molecular typing using recently developed Multilocus Sequence Typing system. The (n = 133) used this study were collected 2010-2016 patients with diagnosed primary or secondary syphilis attending outpatient centers hospitals several locations France. Altogether, 18 different allelic profiles found among fully typed 112). There five variants...
The recently discovered methodologies to cultivate and genetically manipulate Treponema pallidum subsp. (T. pallidum) have significantly helped syphilis research, allowing the in vitro evaluation of antibiotic efficacy, performance controlled studies assess differential treponemal gene expression, generation loss-of-function mutants evaluate contribution specific genetic loci T. virulence. Building on this progress, we engineered SS14 strain express a red-shifted green fluorescent protein...
Abstract The development of therapies and vaccines targeting integral membrane proteins has been complicated by their extensive hydrophobic surfaces, which can make production structural characterization difficult. Here we describe a general deep learning-based design approach for solubilizing native while preserving sequence, fold, function using genetically encoded de novo protein WRAPs ( W ater-soluble R Fdiffused A mphipathic P roteins) that surround the lipid-interacting rendering them...
Treponema pallidum subsp. is the causative agent of syphilis, a sexually transmitted disease with worldwide prevalence. Several different molecular typing schemes are currently available for this pathogen. To enable population biology studies syphilis and epidemiological surveillance at global scale, harmonized tool needs to be introduced. Recently, we published new multi-locus sequence (MLST) potential significantly enhance data in several aspects (e.g., distinguishing genetically clades...
Leptospirosis is a widespread zoonosis with global impact, particularly among vulnerable populations in resource-poor settings tropical countries. Rodents have been considered to be the main reservoir of disease; however, wide variety mammals can act as hosts well. Here we examine genetic diversity Leptospira strains from biological samples patients and animals French Polynesia (FP) 2011 2019.From 2019, collected 444 blood diagnosed having leptospirosis. The limited volume clinical material...
Yaws is an endemic disease caused by Treponema pallidum subsp. pertenue (TPE) that primarily affects children in rural regions of the tropics. The character yaws infections and expected exclusive reservoir TPE humans opened a new opportunity to start eradication campaign. We have developed multi-locus sequence typing (MLST) scheme for isolates combining previously published (TP0548, TP0488) (TP0858) chromosomal loci, we compared this two MLST schemes. applied TPE-containing clinical obtained...
Escherichia albertii is a recently discovered species with limited number of well characterized strains.The aim this study was to characterize four the E. strains, which were among 41 identified strains isolated from feces living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia.Sequencing 16S rDNA, automated ribotyping, rep-PCR used identify isolates.Phylogenetic analyses based multi-locus sequence typing showed these isolates be genetically most similar members...
Abstract Centers for Disease Control and Prevention sequencing-based treponeme typing was used to analyze 72 blood samples, collected from human immunodeficiency virus syphilis co-infected patients during 2014 2015 in Antwerp, Belgium. Twenty-nine (40.3%) isolates were polymerase chain reaction positive Treponema pallidum, all tested macrolide-resistant. Four genotypes identified by including two new genotypes, U4NR8 SU9R8, whereas enhanced revealed 7 subtypes.
A recently introduced Multilocus Sequence Typing scheme for Treponema pallidum subsp. was applied to clinical samples collected from 2004 2017 the two largest cities (Prague and Brno) in Czech Republic. Altogether, a total of 675 were tested this study 281 them found PCR-positive treponemal DNA typeable. Most typed (n = 281) swabs primary or secondary syphilis lesions 231), only minority whole blood tissue 50). Swab patients with rapid plasma regain (RPR) values 1–1024 more frequently...
Treponema pallidum subsp. (TPA) is the infectious agent of syphilis, a disease that infects more than 5 million people annually. Since TPA an uncultivable bacterium, most information on genetics comes from genome sequencing and molecular typing studies. This study presents first complete (without gaps) clinical isolate (UZ1974), which was obtained directly material, without multiplication in rabbits. Whole performed using newly developed Anti-Treponemal Antibody Enrichment technique combined...
The increased prevalence of syphilis in Cuba prompted us to map the circulating Treponema pallidum subsp. allelic profiles this geographic region. Samples were collected from 2012 2017, 83 male patients with ulcers or skin lesions, and examined using multilocus sequence typing. Additionally, we analyzed 23S rDNA 16S regions for presence possible mutations leading macrolide tetracycline resistance. Among 94% fully typed strains, found 7 different profiles, which 4 had not been previously...
Syphilis, caused by Treponema pallidum ssp. (TPA), is a persisting global health problem. Although syphilis diagnostics relies mainly on serology, serological tests have some limitations, and it recommended that the final diagnosis be supported additional tests. The purpose of this study was to analyze relationship between serology PCR in diagnostics. From year 2004 May 2019, total 941 samples were taken from 833 patients suspected having syphilis, Czech Republic. In all these samples, both...
Syphilis, caused by the spirochete Treponema pallidum subspecies pallidum, is a rising global public health concern and laboratory diagnostics remain challenging. Especially during early disease, rapid accurate diagnosis crucial to ensure patients their contacts receive timely treatment eradicate infection prevent further transmission. In this prospective observational study, we evaluated performance of polymerase chain reaction (PCR) serological testing for primary syphilis evaluating...
Abstract In our most recent study, we found that in Tanzania infection with Treponema pallidum ( TP ) subsp. pertenue TPE is present four different monkey species. order to gain information on the diversity and epidemiological spread of Tanzanian nonhuman primates (NHP), identified two suitable candidate genes for multi-locus sequence typing (MLST). We demonstrate functionality MLST system invasively non-invasively collected samples. While were not able frequent interspecies transmission...
Abstract Background The life-threatening pathogen Leptospira interrogans is the most common agent of leptospirosis, an emerging zoonotic disease. However, little known about strains that are currently circulating worldwide due to fastidious nature bacteria and difficulty isolate cultures. In addition, paucity in blood other clinical samples has proven be a considerable challenge for directly genotyping leptospirosis from patient material. Our understanding genetic diversity during human...