A5076039227- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Microbial Metabolism and Applications
- Antimicrobial Resistance in Staphylococcus
- Microbial infections and disease research
- Biochemical and Structural Characterization
- Polar Research and Ecology
- Probiotics and Fermented Foods
- Oral microbiology and periodontitis research
- Plant-Microbe Interactions and Immunity
- Aquaculture disease management and microbiota
- Toxin Mechanisms and Immunotoxins
- Microbial Natural Products and Biosynthesis
- Plant Pathogens and Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Enzyme Production and Characterization
- Molecular Biology Techniques and Applications
- Fungal Biology and Applications
- Listeria monocytogenes in Food Safety
- Enterobacteriaceae and Cronobacter Research
- Microbial Applications in Construction Materials
- Salivary Gland Tumors Diagnosis and Treatment
- Animal Virus Infections Studies
- Escherichia coli research studies
Masaryk University
2015-2025
RECETOX
2025
Brno University of Technology
2024
Central European Institute of Technology
2024
Central European Institute of Technology – Masaryk University
2024
A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part James Ross Island and Eagle Island, Antarctica, 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, exhibited strong hydrolytic activities. To clarify the taxonomic position these isolates, polyphasic approach applied based sequencing 16S rRNA, gyrB lepA genes, whole-genome sequencing, rep-PCR,...
Given the great biological importance and high diversity of temperate Staphylococcus aureus bacteriophages, a method is needed for description their genomic structure. Here we have updated multiplex PCR strategy complex characterization S. phages family Siphoviridae. Based on comparative analysis available phage sequences, multilocus typing major modules genome was designed. The were classified basis genes integrase (10 types), anti-repressor (five replication proteins polA, dnaC dnaD (four...
Four rod-shaped and Gram-stain-negative bacterial strains, CCM 8647, 8649T, 8643T 8648T, were isolated from rock samples collected on James Ross Island, Antarctica. Extensive biotyping, fatty acid profiling, chemotaxonomy, 16S rRNA gene sequencing whole-genome was applied to isolates clarify their taxonomic position. Phylogenetic analysis based indicated that all four belonged the genus Hymenobacter. Strains 8649T 8647 most closely related Hymenobacter arizonensis OR362-8T (94.4 % sequence...
A taxonomic study performed on 17 Gram-stain-negative rod-shaped bacterial strains originating from the Antarctic environment is described. Initial phylogenetic analysis using 16S rRNA gene sequencing differentiated into four groups belonging to genus Pedobacter but they were separated all hitherto described species. Group I (n=8) was closest aquatilis (97.8 % sequence similarity). II (n=2) and group III (n=4) closely related (98.8 similarity) had jejuensis as their common nearest neighbour....
A set of 25 urease-producing, yellow-pigmented enterococci was isolated from environmental sources. Phenotypic classification divided the isolates into two phena. Both phena were characterized using 16S rRNA gene sequence analysis, DNA base composition, rep-PCR fingerprinting and automated ribotyping. The obtained data distinguished all members genus Enterococcus with validly published names placed them in faecalis species group. DNA-DNA hybridization experiments, pheS rpoA sequencing...
Eight Gram-positive, catalase-negative bacterial strains were isolated during screening of enterococcal populations on plants. rep-PCR fingerprinting using the (GTG) 5 primer showed that isolates constituted a single cluster was separate from all known species. 16S rRNA gene sequence phylogenetic analysis three representative belonged to genus Enterococcus and they clustered with faecalis species group. Sequencing genes for phenylalanyl-tRNA synthase alpha subunit ( pheS ) RNA polymerase...
Abstract Objective Chronic otitis media (COM) is a common middle ear disease in children and young adults. Dysfunction of the Eustachian tube bacterial infection are main causes. This pilot study aimed to describe compare bacteriomes adults with serious forms COM, such as cholesteatoma retraction pocket (RP) tympanic membrane, healthy ears. Study Design Observational study. Setting Clinical practice tertiary center. From January 1, 2021 August 31, 2022. Patients aged 0 20 years. Methods In...
Escherichia albertii is a recently discovered species with limited number of well characterized strains.The aim this study was to characterize four the E. strains, which were among 41 identified strains isolated from feces living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia.Sequencing 16S rDNA, automated ribotyping, rep-PCR used identify isolates.Phylogenetic analyses based multi-locus sequence typing showed these isolates be genetically most similar members...
Strain P1297T was isolated in the frame of a project aimed on psychrotolerant microbiota occurring water sources. The strain initially identified as tentative species genus Aeromonas rod-shaped, Gram-stain-negative, facultatively anaerobic and oxidase-positive. Subsequently, 16S rRNA gene sequence analysis placed within class Betaproteobacteria showed Aquitalea magnusonii TRO-001DR8T closest phylogenetic relative with 99.28 % similarity. Digital DDH average nucleotide identity (ANI) were...
A taxonomic study of two fluorescent Pseudomonas strains (HJ/4 T and SJ/9/1 ) isolated from calcite moonmilk samples obtained caves in the Moravian Karst Czech Republic was carried out. Results initial 16S rRNA gene sequence analysis assigned both into genus showed yamanorum 8H1 as their closest neighbour with 99.8 99.7 % similarities to HJ/4 , respectively. Subsequent rpoD rpoB gyrB housekeeping genes confirmed highest similarity isolates P. but phylogeny sequences implied that they are...
A group of four psychrotrophic bacterial strains was isolated on James Ross Island (Antarctica) in 2013. All isolates, originating from different soil samples, were collected the ice-free northern part island. They rod-shaped, Gram-stain-negative, and produced moderately slimy red-pink pigmented colonies R2A agar. polyphasic taxonomic approach based 16S rRNA gene sequencing, whole-genome MALDI-TOF MS, rep-PCR analyses, chemotaxonomic methods extensive biotyping used to clarify position these...
Strain P4487AT was isolated during investigation of cultivable bacterial populations environmental materials sampled at James Ross Island, Antarctica. It revealed Gram-stain-negative short rod-shaped cells producing a pink pigment. Phylogenetic analysis based on 16S rRNA gene sequences allocated strain to the genus Pedobacter but showed that represents distinct intrageneric phylogenetic lineage clearly separated from remaining species. Phylogenetically, formed common branch with arcticus and...
A red-pigmented, Gram-stain-negative, rod-shaped, aerobic bacterium, designated strain CCM 8646T, was isolated from stone fragments in James Ross Island, Antarctica. Strain 8646T able to grow 10 40 °C, the presence of up 1 % (w/v) NaCl and at pH 7.0–11.0. Analysis 16S rRNA gene sequence placed genus Rufibacter with closest relative being roseus H359T (97.07 similarity). The digital DNA–DNA hybridization values between R. were low (21.30±2.34 %). major quinone menaquinone MK-7. polar lipids...
A set of three psychrotrophic bacterial strains was isolated from different soil samples collected at the deglaciated northern part James Ross Island (Antarctica) in 2014. All isolates were rod-shaped, Gram-stain-negative, non-motile, catalase-positive and oxidase-negative, produced moderately slimy red-pink pigmented colonies on Reasoner's 2A (R2A) agar. polyphasic taxonomic approach based 16S rRNA gene sequencing, whole-genome automated ribotyping, MALDI-TOF MS, chemotaxonomy methods...
A bacterial strain designated CCM 8645T was isolated from a soil sample collected nearby mummified seal carcass in the northern part of James Ross Island, Antarctica. The cells were short rods, Gram-stain-negative, non-motile, catalase and oxidase positive, produced red-pink pigment on R2A agar. polyphasic taxonomic approach based 16S rRNA gene sequencing, extensive biotyping using conventional tests commercial identification kits chemotaxonomic analyses applied to clarify its position....
Strains P8930T and 478 were isolated from Antarctic glaciers located on James Ross Island King George Island, respectively. They comprised Gram-stain-negative short rod-shaped cells forming pink pigmented colonies exhibited identical 16S rRNA gene sequences highly similar MALDI TOF mass spectra, hence assigned as representatives of the same species. Phylogenetic analysis based both isolates to genus Pedobacter showed frigidisoli terrae be their closest phylogenetic neighbours, with 97.4 97.2...
Our polyphasic approach provides a new understanding of the taxonomy novel pigmented Rugamonas species isolated from freshwater samples in Antarctica. The isolates showed considerable extracellular bactericidal secretions.
<title>Abstract</title> Three bacterial strains producing blue-violet pigmented colonies on R2A agar were isolated from a wet rock wall and lakes in deglaciated northern part of James Ross Island, Antarctica. The inhibited phytopathogenic Gram-positive bacteria, e.g. Clavibacter spp., Curtobacterium flacumfaciens, Paenarthrobacter ilicis. Phylogenetic analysis based the 16S rRNA gene indicated that isolates belonged to genus Massilia closest relatives violaceinigra B2T, rubra CCM 8692T,...