A5035207920- Berberine and alkaloids research
- Chemical synthesis and alkaloids
- Plant-derived Lignans Synthesis and Bioactivity
- Fungal and yeast genetics research
- Phytochemistry and biological activities of Ficus species
- Alkaloids: synthesis and pharmacology
- Autophagy in Disease and Therapy
- Drug Transport and Resistance Mechanisms
- Microtubule and mitosis dynamics
- Plant tissue culture and regeneration
- Education, Psychology, and Social Research
- Synthesis and bioactivity of alkaloids
- Erythrocyte Function and Pathophysiology
- Fungal Biology and Applications
- Phytochemistry and Biological Activities
- Fermentation and Sensory Analysis
- Plant-based Medicinal Research
- Cell death mechanisms and regulation
- Chemotherapy-induced cardiotoxicity and mitigation
- Botanical Research and Chemistry
- Natural product bioactivities and synthesis
- Pluripotent Stem Cells Research
- Plant Reproductive Biology
- Blood properties and coagulation
- DNA and Nucleic Acid Chemistry
Masaryk University
2014-2024
Various types of feeder cells have been adopted for the culture human embryonic stem (hESCs) to improve their attachment and provide them with stemness-supporting factors. However, differ in capacity support growth undifferentiated hESCs. Here, we compared expression secretion four well-established regulators hESC pluripotency and/or differentiation among five lines foreskin fibroblasts primary mouse throughout a standard procedure. We found that secreted comparable levels TGFbeta1. larger...
Although colonies from Saccharomyces cerevisiae laboratory strains are smooth, those isolated nature exhibit a structured fluffy pattern. Environmental scanning electron microscopy shows that the cells within wild connected by extracellular matrix (ECM) material. This material contains protein of about 200 kDa unrelated to flocculins, proteins involved in cell-cell adhesion liquid media. The binds concanavalin A. Within few passages on rich agar medium, switch smooth colony morphology....
ABSTRACT Neural rosette formation is a critical morphogenetic process during neural development, whereby stem cells are enclosed in niches to equipoise proliferation and differentiation. How rosettes form provide regulatory micro-environment remains be elucidated. We employed the human embryonic cell-based system investigate structural development function of rosettes. Our study shows that consists five types morphological change: intercalation, constriction, polarization, elongation lumen...
Multidrug resistance (MDR) is a common problem when fighting cancer with chemotherapy. P-glycoprotein (P-gp, or MDR1) an active pump responsible for the efflux of xenobiotics out cell, including anti-cancer drugs. It validated target against MDR. No crystal structure human P-gp available to date, and only recently several cryo-EM structures have been solved. In this paper, we present comprehensive computational approach that includes constructing full-length three-dimensional its refinement...
The phenolic compounds of methanolic extracts Salvia pomifera and fruticosa were identified by liquid chromatography tandem mass spectrometry. Carnosic acid its metabolite carnosol the most abundant terpene S. fruticosa, while they completely absent in pomifera. main constituent was 12-O-methylcarnosic mass/mass fragmentation pathway explained. detailed mechanism carnosic oxidation to suggested. effects and/or acid, diterpene component on proliferation cell cycle two melanoma lines (A375,...
Abstract We show that the plant quaternary benzo[ c ]phenanthridine alkaloid sanguilutine (SL) is a strong inducer of caspase-independent non-apoptotic death in human melanoma cells. Necrostatin-1, specific inhibitor necroptosis, completely reversed cytotoxic effect SL, suggesting necroptosis was predominant type cell induced by SL these In addition, we showed can trigger an autophagic response, as confirmed GFP-LC3 puncta formation and LC3-II accumulation. Interestingly, observed...
Tumor suppressor p53 is mutated in about 50% of cancers. Most malignant melanomas carry wild-type p53, but activity often inhibited due to overexpression its negative regulators Mdm2 or MdmX. We performed high throughput screening 2448 compounds on A375 cells carrying luciferase reporter construct reveal that promote melanoma. Albendazole and fenbendazole, two approved commonly used benzimidazole anthelmintics, stimulated were selected for further studies. The protein levels p21 increased...
Abstract Background: Quaternary benzo[c]phenanthridine alkaloids (QBAs) are naturally occurring compounds isolated from plants in the Fumariaceae, Papaveraceae, Ranunculaceae, and Rutaceae families. In addition to having a wide range of biological activities, they also attractive for their fluorescent properties. We observed interesting characteristics QBAs–macarpine (MA), sanguirubine (SR), chelirubine (CHR), sanguilutine (SL), chelilutine (CHL), sanguinarine (SA) chelerythrine (CHE) after...
Escherichia albertii is a recently discovered species with limited number of well characterized strains.The aim this study was to characterize four the E. strains, which were among 41 identified strains isolated from feces living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia.Sequencing 16S rDNA, automated ribotyping, rep-PCR used identify isolates.Phylogenetic analyses based multi-locus sequence typing showed these isolates be genetically most similar members...
Haloperidol is a neuroleptic drug used for medication of various psychoses and deliria. Its administration frequently accompanied by cardiovascular side effects, expressed as QT interval prolongation occurrence even lethal arrhythmias. Despite these haloperidol still prescribed in Europe clinical practice. binds to sigma receptors that are coupled with inositol 1,4,5-trisphosphate (IP3) receptors. Sigma tissues, including heart muscle, they modulate potassium channels. Together IP3...
Quaternary benzo(c.)phenanthridine alkaloids are a relatively small group of isoquinoline with attractive biological activities. They produced by number plant species the Papaveraceae, Fumariaceae, and Rutaceae families. Differential cytotoxicity minor, naturally occurring derivatives sanguirubine, chelirubine, macarpine better known benzophenanthridines sanguinarine chelerythrine in cancer normal cells was assessed vitro. MTT assay using panel consisting either transformed cell lines (HeLa;...
The aim of the present study was to determine structural requirements for dibenzocyclooctadiene lignans essential P-glycoprotein inhibition. Altogether 15 structurally related isolated from Schisandra chinensis or prepared by modification their backbone were investigated, including three pairs enantiomers. P-Glycoprotein inhibition quantified using a doxorubicin accumulation assay in human promyelotic leukemia HL60/MDR cells overexpressing P-glycoprotein. A preliminary quantitative...
Lichens are resilient organisms, known for their unique profiles of secondary metabolites and exhibiting antioxidative, antibacterial, cytotoxic effects. Analyzing the potential Lobaria scrobiculata, a bioassay-guided fractionation strategy yielded seven metabolites, with two these compounds, 2 3, cytotoxicity against HL-60 cells. In order to verify impact degradation on observed bioactivity, purity stability evaluation was conducted. The consistency results obtained by water-soluble...
BRAF inhibitors can delay the progression of metastatic melanoma, but resistance usually emerges, leading to relapse. Drugs simultaneously targeting two or more pathways essential for cancer growth could slow prevent development resistant clones. Here, we identified pyridinyl imidazole compounds SB202190, SB203580, and SB590885 as dual critical proliferative in human melanoma cells bearing V600E activating mutation kinase. We found that drugs disrupt V600E-driven extracellular...