Our understanding of human gut microbiota in health and disease depends on accurate reproducible microbial data acquisition. The critical step this process is to apply an appropriate methodology extract DNA, since biases introduced during the DNA extraction may result inaccurate representation. In study, we attempted find a protocol which could be effectively used analyze both bacterial fungal community. We evaluated effect five methods (QIAamp Stool Mini Kit, PureLinkTM Microbiome...

Relationships between conformation traits and functional longevity in Holstein cows were evaluated using survival analysis. Functional was defined as the number of days first calving culling; that is, length productive life. The data set consisted 116,369 calved from 2003 to 2008. All used analysis scored for d 30 210 their lactation. included 48% censored records. Analyses done separately 20 linear descriptive type traits, 6 composite height at sacrum measured centimeters. Cox proportional...

Common Variable Immunodeficiency (CVID) is the most frequent symptomatic immune disorder characterized by reduced serum immunoglobulins. Patients often suffer from infectious and serious non-infectious complications which impact their life tremendously. The monogenic cause has been revealed in a minority of patients so far, indicating role multiple genes environmental factors CVID etiology. Using 16S ITS rRNA amplicon sequencing, we analyzed bacterial fungal gut microbiota, respectively,...

The objectives of this study were to estimate genetic parameters for 18 currently recorded linear conformation traits in Czech Holstein cattle. data set consisted 78 886 first-lactating cows. Traits, all scored on a 9-point scale, fore udder attachment, rear height, depth, width, central ligament, teat length, front placement, position, stature, angularity, chest body rump angle, legs view, leg (side view), foot and bone quality. Uni- bivariate animal models used. included fixed effects...

To determine whether contrast-enhanced ultrasonography (CEUS) as the first-line method is more cost-effective in evaluating incidentally discovered focal liver lesions (FLLs) than computed tomography (CT) and magnetic resonance imaging (MRI).

The relationships between conformation and longevity traits were analysed in 58 493 Czech Fleckvieh cows first calved from 1994 to 2003. All scored for during the lactation. Genetic correlations estimated by bivariate runs using VCE 4.0 program variance component estimation. values of heritability range 0.06 0.63 0.04 0.05. Low or intermediate genetic recorded linear trait found. lower functional longevity. Body measurements showed negative with real as well (-0.12 -0.29). dairy character...

An increasing trend in non albicans infections and various susceptibility patterns to antifungal agents implies a requirement for the quick reliable identification of number medically important Candida species. Real-time PCR followed by high resolution melting analysis (HRMA) was developed, tested on 25 reference collection strains validated an additional 143 clinical isolates this study. All inconclusive when using phenotypic methods and/or HRMA were analysed ITS2 sequencing. Considering...

The relationships between conformation traits and longevity were analyzed in 41 489 Holstein cows born the years 1994-1999 which culled by 30th June 2005. Pearson correlation coefficients type herd life or productive of calculated. effects trait scoring level on length described means analyses variance. observed correlations are range -0.061 to 0.160. Negative found for rump angle, rear leg set, udder depth, teat length. Most body had slightly positive life, indicating that larger live...

The phenotypic relationships between type traits and functional were analyzed in 47 786 Czech Fleckvieh cows first calved from 1994 to 2003. Functional survival was defined as the number of days calving culling. All scored for conformation during lactation. Type information consisted scores 17 objectively linear (with 9 classes each) measurements 6 body (measured cm). impact chosen on longevity estimated using Cox proportional hazards model. statistical model included combined time-dependent...

A taxonomic study of two fluorescent Pseudomonas strains (HJ/4 T and SJ/9/1 ) isolated from calcite moonmilk samples obtained caves in the Moravian Karst Czech Republic was carried out. Results initial 16S rRNA gene sequence analysis assigned both into genus showed yamanorum 8H1 as their closest neighbour with 99.8 99.7 % similarities to HJ/4 , respectively. Subsequent rpoD rpoB gyrB housekeeping genes confirmed highest similarity isolates P. but phylogeny sequences implied that they are...

High-resolution melting analysis (HRMA) is a fast (post-PCR) high-throughput method to scan for sequence variations in target gene. The aim of this study was test the potential HRMA distinguish particular bacterial species Staphylococcus genus even when using broad-range PCR within 16S rRNA gene where differences are minimal. Genomic DNA samples isolated from 12 reference staphylococcal strains ( aureus , capitis caprae epidermidis haemolyticus hominis intermedius saprophyticus sciuri...

ABSTRACT Early diagnosis of fungal infection is critical for initiating antifungal therapy and reducing the high mortality rate in immunocompromised patients. In this study, we focused on rapid sensitive identification clinically important Candida species, utilizing variability length ITS2 rRNA gene fluorescent capillary electrophoresis (f- -PCR-CE). The method was developed optimized 29 various reference strains from which 26 species were clearly identified, while guilliermondii , C....

Soubor nemocných a metodika: Do sledování bylo zařazeno 70 s čerstvě vzniklou dilatační kardiomyopatií (DKMP), ejekční frakcí levé komory (EFLK) < 40 % dobou trvání symptomů kratší než 12 měsíců. Ve vzorcích z endomyokardiální biopsie (EMB) byl stanoven průměrný počet T-lymfocytů (CD3+ buněk) mononukleárních leukocytů (LCA+ v mm2. Přítomnost zánětu byla hodnocena jako pozitivní případě nálezu > 7 CD3+ buněk a/nebo 14 LCA+ Detekce patologických agens myokardu provedena metodou kvantitativní...

Background Segmentation of the gray and white matter (GM, WM) human spinal cord in MRI images as well analysis diffusivity are challenging. When appropriately segmented, diffusion tensor imaging (DTI) might be beneficial diagnosis prognosis several diseases. Purpose To evaluate applicability a semiautomatic algorithm provided by ITK‐SNAP classification mode (CLASS) for segmenting cervical GM, WM analyzing DTI parameters. Study Type Prospective. Subjects Twenty healthy volunteers. Sequences...

Summary Crossbreeding effects on milk production traits of Czech dual‐purpose and dairy cattle breeds were estimated. Nearly 370 000 cows with known gene proportions from Pied, Ayrshire or Holstein selected the national recording data base. Single‐trait animal models calculated for milk, fat protein yield, content. The model Dickerson including additive, additive maternal, heterotic recombination was used part describing crossbreeding in all calculations. For genetic effect (defined as...

Introduction: Viral infections are considered the most frequent cause of myocarditis and dilated cardiomyopathy (DCM).Material methods: We investigated changes in viral presence impact genome persistence myocardium on echocardiographic parameters, functional status some laboratory parameters a 6-month follow-up.Fifty-four patients with recent onset DCM, left ventricular ejection fraction < 40% biopsy-proven (> 14 mononuclear leukocytes/mm 2 and/or > 7 T-lymphocytes/mm ) were...

The investigation was based on roughly 3.9, 2.7 and 1.7 million test-day records from first, second third lactation, respectively, sampled 596 200 Czech Holstein cows between the years 1991 2002. Breeding values were estimated multi-lactation random-regression models which contained fixed effect of herd-test day, regression days in milk random regressions animal level permanent environmental effect. Third degree Legendre polynomials (with four coefficients) used for both regressions....

Introduction Urokinase receptor (uPAR, CD87), a glycosylphosphatidylinositol-anchored protein, is considered to play an important role in inflammation and fibrinolysis.The Gram-negative bacterium Burkholderia pseudomallei able survive replicate within leukocytes causes melioidosis, cause of pneumonia-derived community-acquired sepsis Southeast Asia.We here investigated the expression function uPAR both patients with septic melioidosis murine model experimental melioidosis.Methods Using...

In Europe, peripartum cardiomyopathy (PPCM) is a rare disorder, often difficult to diagnose and it has variable clinical course. The aim of this report was describe discuss the individual variability disorder its management.Three cases PPCM manifesting as severe heart failure are compared. Common presence myocardial inflammation detected by endomyocardial biopsy. Different were treatment methods Modern therapeutic concepts such immunosuppressive therapy bromocriptin administration discussed,...

The relationship between type traits in Holstein cows over generations was analysed. analysed data set included a total of 12 157 dam-daughter pairs with linear scores 14 including final score and general characteristics type. Significant relationships existed dams their daughters. Pearson correlation coefficients ranged from r = 0.20 to 0.63 except the rump angle. A negative (r -0.02) found for this trait. similar tendency resulted analysis values score.