A5000797815- Enzyme Production and Characterization
- Enzyme Structure and Function
- Neuroscience and Neuropharmacology Research
- Chemical Synthesis and Analysis
- Receptor Mechanisms and Signaling
- Enzyme-mediated dye degradation
- Amino Acid Enzymes and Metabolism
- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Microbial Metabolites in Food Biotechnology
- Epigenetics and DNA Methylation
- Glycosylation and Glycoproteins Research
- Contact Dermatitis and Allergies
- Nicotinic Acetylcholine Receptors Study
- Crystal structures of chemical compounds
- Molecular Sensors and Ion Detection
- Biochemical and Molecular Research
- Crystallography and molecular interactions
- Histone Deacetylase Inhibitors Research
- Analytical Chemistry and Chromatography
- Monoclonal and Polyclonal Antibodies Research
- Insect and Pesticide Research
- Carbohydrate Chemistry and Synthesis
- Cancer-related gene regulation
- Phytase and its Applications
University of Copenhagen
2016-2025
Saniona (Denmark)
2015
NeuroSearch (Denmark)
2015
The University of Sydney
2015
University of Montana
2015
Emory University
2007-2015
IT University of Copenhagen
2008
Chinese University of Hong Kong
2008
Novozymes (Denmark)
2008
Lundbeck (Denmark)
2007
Although amyloid fibrillation is generally believed to be a nucleation-dependent process, the nuclei are largely structurally uncharacterized. This in part due inherent experimental challenge associated with structural descriptions of individual components dynamic multi-component equilibrium. There indications that oligomeric aggregated precursors fibrillation, and not mature fibrils, main cause cytotoxicity disease. further emphasizes importance characterizing early events. Here we present...
We have solved the x-ray structures of binary horseradish peroxidase C-ferulic acid complex and ternary C-cyanide-ferulic to 2.0 1.45 Å, respectively. Ferulic is a naturally occurring phenolic compound found in plant cell wall an <i>in vivo</i>substrate for peroxidases. The demonstrate flexibility dynamic character aromatic donor binding site emphasize role distal arginine (Arg<sup>38</sup>) both substrate oxidation ligand binding. Arg<sup>38</sup> hydrogen bonds bound cyanide, thereby...
Abstract The symptoms characteristic of allergic hypersensitivity are caused by the release mediators, i.e., histamine, from effector cells such as basophils and mast cells. Allergens with more than one B cell epitope cross-link IgE Abs bound to high affinity FcεRI receptors on surfaces leading aggregation subsequent mediator release. Thus, allergen-Ab complexes play a crucial role in cascade response. We here report structure 1:1 complex between major birch pollen allergen Bet v 1 Fab...
The three-dimensional structure of recombinant horseradish peroxidase in complex with BHA (benzhydroxamic acid) is the first a peroxidase-substrate demonstrating existence an aromatic binding pocket. crystal has been determined to 2.0 A resolution crystallographic R-factor 0.176 (R-free = 0. 192). well-defined electron density for observed active site, hydrophobic pocket surrounding ring substrate. provided by residues H42, F68, G69, A140, P141, and F179 heme C18, C18-methyl, C20, shortest...
Inhibition of the ternary protein complex synaptic scaffolding postsynaptic density protein-95 (PSD-95), neuronal nitric oxide synthase (nNOS), and N -methyl- d -aspartate (NMDA) receptor is a potential strategy for treating ischemic brain damage, but high-affinity inhibitors are lacking. Here we report design synthesis novel dimeric inhibitor, Tat- PEG4(IETDV) 2 (Tat- -dimer), which binds tandem PDZ1-2 domain PSD-95 with an unprecedented high affinity 4.6 nM, displays extensive...
Abstract Specific allergy vaccination is an efficient treatment for allergic disease; however, the development of safer vaccines would enable a more general use treatment. Determination molecular structures allergens and allergen-Ab complexes facilitates epitope mapping enables rational approach to engineering allergen molecules with reduced IgE binding. In this study, we describe identification modification human IgE-binding based on crystal structure Bet v 1 in complex BV16 Fab′ fragment....
Abstract Ves v 5 is one of three major allergens found in yellow‐jacket venom: phospholipase A 1 (Ves 1), hyaluronidase 2), and antigen 5). related by high amino acid sequence identity to pathogenesis‐related proteins including from mammals, reptiles, insects, fungi, plants. The crystal structure has been solved refined a resolution 1.9 Å. majority residues conserved between the can be rationalized terms hydrogen bonding patterns hydrophobic interactions defining an α‐β‐α sandwich core...
The orphan glutamate-like receptor GluRδ2 is predominantly expressed in Purkinje cells of the central nervous system. classification to ionotropic glutamate family based on sequence similarities, because does not form functional homomeric glutamate-gated ion channels transfected cells. Studies −/− knockout mice as well with naturally occurring mutations gene have demonstrated an essential role cerebellar long-term depression, motor learning, coordination, and synaptogenesis. However, lack a...
Soybean seed coat peroxidase (SBP) is a with extraordinary stability and catalytic properties. It belongs to the family of class III plant peroxidases that can oxidize wide variety organic inorganic substrates using hydrogen peroxide. Because enzyme heterogeneous glycoprotein, SBP was produced recombinant in Escherichia coli for present crystallographic study. The three-dimensional structure shows bound tris(hydroxymethyl)aminomethane molecule (TRIS). This TRIS has bonds active site residues...
Targeting the protein–protein interaction (PPI) between nuclear factor erythroid 2-related 2 (Nrf2) and Kelch-like ECH-associated protein 1 (Keap1) is a potential therapeutic strategy to control diseases involving oxidative stress. Here, six classes of known small-molecule Keap1–Nrf2 PPI inhibitors were dissected into 77 fragments in fragment-based deconstruction reconstruction (FBDR) study tested four orthogonal assays. This gave 17 fragment hits which shown by X-ray crystallography bind...
Targeting the protein–protein interaction (PPI) between transcription factor nuclear erythroid 2-related 2 (Nrf2) and its repressor, Kelch-like ECH-associated protein 1 (Keap1), constitutes a promising strategy for treating diseases involving oxidative stress inflammation. Here, fragment-based drug discovery (FBDD) campaign resulted in novel, high-affinity (Ki = 280 nM), cell-active noncovalent small-molecule Keap1-Nrf2 PPI inhibitors. We screened 2500 fragments using orthogonal...
Amylosucrase from Neisseria polysaccharea is a remarkable transglucosidase family 13 of the glycoside-hydrolases that synthesizes an insoluble amylose-like polymer sucrose in absence any primer. shares strong structural similarities with alpha-amylases. Exactly how this enzyme catalyzes formation alpha-1,4-glucan and which features are involved unique functionality existing important questions still not fully answered. Here, we provide evidence amylosucrase initializes by releasing, through...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMarine alkaloids. 10. Chartelline A: a pentahalogenated alkaloid from the marine bryozoan Chartella papyraceaLionel Chevolot, Anne Marie Michael Gajhede, Charles Larsen, Uffe Anthoni, and Carsten ChristophersenCite this: J. Am. Chem. Soc. 1985, 107, 15, 4542–4543Publication Date (Print):July 1, 1985Publication History Published online1 May 2002Published inissue 1 July...
Around 80 enzymes are implicated in the generic starch and sucrose pathways. One of these is phosphorylase, which reversibly catalyzes conversion orthophosphate to d-Fructose α-d-glucose 1-phosphate. Here, we present crystal structure phosphorylase from Bifidobacterium adolescentis (BiSP) refined at 1.77 Å resolution. It represents first 3D a phosphate-dependent enzyme glycoside hydrolase family 13. The BiSP composed four domains A, B, B', C. Domain A comprises (β/α)8-barrel common catalytic...
The X‐ray structure of the ligand‐binding core kainate receptor GluR5 (GluR5‐S1S2) in complex with ( S )‐glutamate was determined to 1.95 Å resolution. overall GluR5‐S1S2 comprises two domains and is similar related AMPA GluR2‐S1S2J. binds as Distinct features are observed for Ser741, which stabilizes a highly coordinated network water molecules forms an interdomain bridge. exhibits high degree domain closure (26°) relative apo In addition, novel dimer interface different arrangement...
The reaction mechanism of sucrose phosphorylase from Bifidobacterium adolescentis (BiSP) was studied by site-directed mutagenesis and x-ray crystallography. An inactive mutant BiSP (E232Q) co-crystallized with sucrose. structure revealed a substrate-binding mode comparable that seen in other related sucrose-acting enzymes. Wild-type also crystallized the presence In dimeric structure, covalent glucosyl intermediate formed one molecule dimer, after hydrolysis intermediate, beta-D-glucose...
Dynamic methylations and demethylations of histone lysine residues are important for gene regulation facilitated by methyltransferases demethylases (HDMs). KDM5B/Jarid1B/PLU1 is an H3K4me3/me2-specific demethylase belonging to the JmjC domain-containing family (JHDMs). Several studies have linked KDM5B breast, prostate skin cancer, highlighting its potential as a drug target. However, most inhibitor focused on other JHDMs, inhibitors remain be explored. Here, we report expression,...
Abstract The metzincin metalloproteinase PAPP-A plays a key role in the regulation of insulin-like growth factor (IGF) signaling by specific cleavage inhibitory IGF binding proteins (IGFBPs). Using single-particle cryo-electron microscopy (cryo-EM), we here report structure complex with its endogenous inhibitor, stanniocalcin-2 (STC2), neither which have been reported before. highest resolution (3.1 Å) was obtained for STC2 subunit and N-terminal approximately 1000 residues subunit. 500 kDa...