A5101436790- Enzyme-mediated dye degradation
- Occupational exposure and asthma
- Agricultural safety and regulations
- Metal-Catalyzed Oxygenation Mechanisms
- Porphyrin Metabolism and Disorders
- Contact Dermatitis and Allergies
- Biochemical and biochemical processes
- Estrogen and related hormone effects
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Pesticide and Herbicide Environmental Studies
- Effects and risks of endocrine disrupting chemicals
- Hormonal Regulation and Hypertension
- Air Quality and Health Impacts
- Food Allergy and Anaphylaxis Research
- Hemoglobin structure and function
- Electrochemical sensors and biosensors
- Environmental Toxicology and Ecotoxicology
- Enzyme Production and Characterization
- Influenza Virus Research Studies
- Electrochemical Analysis and Applications
- Biochemical and Structural Characterization
- Respiratory viral infections research
- Metalloenzymes and iron-sulfur proteins
Moffitt Cancer Center
2017-2024
Urbana University
2023
University of Illinois Urbana-Champaign
2023
University of Bath
2005-2021
Griffith University
2016-2020
University of Rochester
2019-2020
RMIT University
2014-2020
Ithaca College
2019
University of San Diego
2018
University of Rochester Medical Center
2018
A synthetic gene encoding horseradish peroxidase isoenzyme C (HRP C) has been synthesized and expressed in Escherichia coli. The nonglycosylated recombinant enzyme C*) was produced inclusion bodies an insoluble inactive form containing only traces of heme. HRP C* solubilized conditions under which it folded to give active were determined. Folding shown be critically dependent upon the concentrations urea, Ca2+, heme on oxidation by oxidized glutathione. Purification from folding mixture gave...
It has been shown recently that Trp171 of lignin peroxidase (LiP) is hydroxylated at the Cβ position [Blodig, W., Doyle, W. A., Smith, A. T., Winterhalter, K., Choinowski, and Piontek, K. (1998) Biochemistry 37, 8832−8838]. Comparative experiments, carried out on both wild-type fungal recombinant LiP isoenzyme H8 (LiPH8*), indicate process hydroxylation autocatalytic may be implicated in catalysis. The role this residue therefore examined using site-directed mutagenesis to obtain enzymes...
We have solved the x-ray structures of binary horseradish peroxidase C-ferulic acid complex and ternary C-cyanide-ferulic to 2.0 1.45 Å, respectively. Ferulic is a naturally occurring phenolic compound found in plant cell wall an <i>in vivo</i>substrate for peroxidases. The demonstrate flexibility dynamic character aromatic donor binding site emphasize role distal arginine (Arg<sup>38</sup>) both substrate oxidation ligand binding. Arg<sup>38</sup> hydrogen bonds bound cyanide, thereby...
The three-dimensional structure of recombinant horseradish peroxidase in complex with BHA (benzhydroxamic acid) is the first a peroxidase-substrate demonstrating existence an aromatic binding pocket. crystal has been determined to 2.0 A resolution crystallographic R-factor 0.176 (R-free = 0. 192). well-defined electron density for observed active site, hydrophobic pocket surrounding ring substrate. provided by residues H42, F68, G69, A140, P141, and F179 heme C18, C18-methyl, C20, shortest...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease that lacks effective biomarkers for early detection. We hypothesized circulating long non-coding RNAs (lncRNAs) may act as diagnostic markers of incidentally-detected cystic PDAC precursors known intraductal papillary mucinous neoplasms (IPMNs) and predictors their pathology/histological classification. Using NanoString nCounter® technology, we measured the abundance 28 candidate lncRNAs in pre-operative plasma from a...
Lactiplantibacillus plantarum B21 isolated from Vietnamese sausage (nem chua) has previously displayed broad antimicrobial activity against Gram-positive bacteria including foodborne pathogens Listeria monocytogenes and Clostridium perfringens. This study successfully identified the agent as plantacyclin B21AG, a 5668 Da circular bacteriocin demonstrating high thermostability, resistance to wide range of pH, proteolytic temporal stability. We report reverse genetics approach identify...
Lignin peroxidase (LiP) plays a central role in the biodegradation of plant cell wall constituent lignin. LiP is able to oxidize aromatic compounds with redox potentials higher than 1.4 V (NHE) by single electron abstraction, but exact mechanism still poorly understood. The finding our laboratory that Cβ-atom Trp171 carries unique modification led us initiate experiments investigate this residue. These experiments, employing crystallography, site-directed mutagenesis, protein chemistry,...
A horseradish peroxidase variant ([F41V] HRP‐C * ), in which Val replaces the conserved Phe at position 41 adjacent to distal His, has been constructed. Its composition and spectroscopic, catalytic substrate‐binding properties were compared with those of wild type recombinant (HRP‐C ) plant (HRP‐C) enzymes. Presteady‐state kinetic measurments rate constant for compound I formation ( k 1 revealed an eightfold decrease reactivity Phe41→Val towards H 2 O , comparison or . Measurement remaining...
Benefits and costs of dispersal philopatry the social plateau pika (Ochotona curzoniae) were studied on Tibetan for 3 years. Although short-lived, pikas live in cohesive family groups that occupy burrow systems sedge meadow habitat Most (57.8%) philopatric, movements extremely restricted. No juvenile females or adult moved more than two ranges between years; greatest observed distances by males five from their birth. Traversing unfamiliar was not a cost because most dispersers settled...
An engineered cDNA from Phanerochaete chrysosporium encoding both the mature and pro-sequence regions of Lip isoenzyme H8 (Lip) has been successfully overexpressed in Escherichia coli. The recombinant protein (LipP*) was sequestered inclusion bodies. reduced–denatured polypeptide purified by differential solubilization, active enzyme recovered after controlled vitro refolding (albeit low yield), glutathione-mediated oxidation disulphides, a folding medium containing an intermediate...
The manner in which the distal heme pocket residues of peroxidases control reaction mechanism and ligand binding has been investigated further by analysis electronic absorption resonance Raman (RR) spectra site mutants recombinant horseradish peroxidase (HRP-C*). roles conserved histidine arginine residues, particularly context catalytic originally proposed for cytochrome c (CCP), have evaluated studying His42 → Leu, Arg, Arg38 Gly, Leu variants HRP-C*. Spectra ferric forms, their complexes...
The anticancer activities and SARs of estradiol-17-O-sulfamates estradiol 3,17-O,O-bis-sulfamates (E2bisMATEs) as steroid sulfatase (STS) inhibitors antiproliferative agents are discussed. Estradiol 20 21, in contrast to the 17-O-monosulfamate 11, proved be excellent STS inhibitors. 2-Substituted E2bisMATEs 21 23 additionally exhibited potent activity with mean graph midpoint values 18-87 nM NCI 60-cell-line panel. Exhibited antiangiogenic vitro vivo an early-stage Lewis lung model, dosed...
This publication is linked to the following EFSA Supporting Publications article: http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2017.EN-1224/full
By introducting the steroid sulfatase inhibitory pharmacophore into aromatase inhibitor 1 (YM511), two series of single agent dual aromatase−sulfatase inhibitors (DASIs) were generated. The best DASIs in vitro (JEG-3 cells) are 5, (IC50(aromatase) = 0.82 nM; IC50(sulfatase) 39 nM), and 14, 0.77 590 nM). X-ray crystallography docking studies selected compounds an homology model crystal structure presented. Both 5 14 inhibit PMSG pretreated adult female Wistar rats potently 3 h after a oral 10...
The surface oxidation site (Trp-171) in lignin peroxidase (LiP) required for the reaction with veratryl alcohol a high-redox-potential (1.4 V) substrate, was engineered into Coprinus cinereus (CiP) by introducing Trp residue heme that has similar protein fold but lacks this activity. To create catalytic activity toward CiP, it necessary to reproduce and its negatively charged microenvironment means of triple mutation. resulting D179W+R258E+R272D variant characterized multifrequency EPR...