A5082369012- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- MicroRNA in disease regulation
- Developmental Biology and Gene Regulation
- Advanced biosensing and bioanalysis techniques
- Student Assessment and Feedback
- Congenital heart defects research
- Virus-based gene therapy research
- Animal Genetics and Reproduction
- RNA modifications and cancer
- Evaluation of Teaching Practices
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Viral Infectious Diseases and Gene Expression in Insects
- Renal and related cancers
- Reflective Practices in Education
- Photosynthetic Processes and Mechanisms
- COVID-19 Clinical Research Studies
University of Birmingham
2018-2023
University of Oxford
2014-2023
MRC Weatherall Institute of Molecular Medicine
2023
Genomics (United Kingdom)
2021
Centre for Human Genetics
2020
Cardiff Metropolitan University
2015
University of Cambridge
2011-2012
The Gurdon Institute
2011
Abstract Most metazoan embryos commence development with rapid, transcriptionally silent cell divisions, genome activation delayed until the mid-blastula transition (MBT). However, a set of genes escapes global repression and gets activated before MBT. Here we describe formation spatio-temporal dynamics pair distinct transcription compartments, which encompasses earliest gene expression in zebrafish. 4D imaging pri- miR430 zinc-finger-gene activities by novel, native approach reveals...
In anamniote embryos, the major wave of zygotic genome activation starts during mid-blastula transition. However, some genes escape global repression, are activated substantially earlier, and contribute to minor activation. The mechanisms underlying little understood. We explored genomic organization cis-regulatory a transcription body, in which is first detected zebrafish. identified miR-430 cluster as having excessive copy number highest density Pol-II-transcribed promoters genome, this...
Abstract MicroRNA (miRNA) target recognition is largely dictated by short ‘seed’ sequences, and single miRNAs therefore have the potential to regulate a large number of genes. Understanding contribution specific miRNA–target interactions regulation biological processes in vivo remains challenging. Here we use transcription activator-like effector nuclease (TALEN) clustered regularly interspaced palindromic repeat (CRISPR)/Cas9 technologies interrogate functional relevance predicted miRNA...
In many animal models, primordial germ cell (PGC) development depends on maternally deposited plasm, which prevents somatic fate. Here, we show that PGCs respond to regulatory information from the plasm in two distinct phases using mechanisms zebrafish. We demonstrate commence zygotic genome activation together with blastocysts no demonstrable differences transcriptional and chromatin opening. Unexpectedly, both PGC activate germ-cell-specific genes, are only stabilized by cytoplasmic...
Abstract The Drosophila melanogaster anterior–posterior axis is established during oogenesis by the localization of bicoid and oskar mRNAs to anterior posterior poles oocyte. Although genetic screens have identified some trans-acting factors required for these transcripts, other may been missed because they also function at stages oogenesis. To circumvent this problem, we performed a screen revertants dominant suppressors bicaudal phenotype caused expressing Miranda–GFP in female germline....
Summary Dgp71WD/Nedd1 proteins are essential for mitotic spindle formation. In human cells, Nedd1 targets γ-tubulin to both centrosomes and spindles, but in other organisms the function of is less clear. Drosophila Dgp71WD plays a major part targeting not centrosomes, while Xenopus egg extracts, acts as more general microtubule (MT) organiser that can independently γ-tubulin. The interpretation these studies, however, complicated by fact some residual likely present cells/extracts analysed....
Genome sequencing is a powerful tool for identifying SARS-CoV-2 variant lineages; however, there can be limitations due to sequence dropout when used identify specific key mutations. Recently, ThermoFisher Scientific has developed genotyping assays help bridge the gap between testing capacity and capability generate real-time results based on variants.
Students construct meaning through relevant learning activities (Biggs, 2003) which are largely determined by the type, amount, and timing of feedback (Carless, 2006). The aim present study was to develop a greater awareness understanding formative assessment practices their relationship with learning. During 2011 five focus group discussions were undertaken students academic staff involved range modules degree pathways at UK University. Three groups undergraduate (one each level study), one...
Gene expression studies and gene therapy require efficient delivery into cells. Different technologies by viral non-viral mechanisms have been used for Small vectors transfer across the cell membrane with a relatively high efficiency, but not large genes or entire loci spanning several kilobases, which do remain intact following introduction. Previously, we developed an system based on herpes virus simplex type 1 (HSV-1) amplicons to fragments of DNA incorporated in human artificial...
Abstract Developmental studies have revealed the importance of transcription factor Hand2 in cardiac development. promotes progenitor differentiation and epithelial maturation, while repressing other tissue types. The mechanisms underlying promotion fates are far better understood than those repression alternative fates. Here, we assess Hand2-dependent changes gene expression chromatin remodeling progenitors zebrafish embryos. Cell-type specific transcriptome analysis shows a dual function...
Abstract Most metazoan embryos commence development with rapid cleavages without zygotic gene expression and their genome activation is delayed until the mid-blastula transition (MBT). However, a set of genes escape global repression during extremely fast cell cycles, which lack gap phases transcription activated before MBT. Here we describe formation spatio-temporal dynamics distinct compartment, encompasses earliest detectable first wave activation. Simultaneous 4D imaging pri-miR430 zinc...
ABSTRACT In anamniote embryos the major wave of zygotic genome activation (ZGA) starts during mid-blastula transition. This ZGA is facilitated by several mechanisms, including dilution repressive maternal factors and accumulation activating transcription fast cell division cycles preceding However, a set genes escape global repression are activated substantially earlier, what called, minor activation. While mechanisms underlying have been studied extensively, little understood. zebrafish...
ABSTRACT Genome sequencing is a powerful tool for identifying SARS-CoV-2 variant lineages, however there can be limitations due to sequence drop-out when used identify specific key mutations. Recently, Thermo Fisher Scientific have developed genotyping assays help bridge the gap between testing capacity and capability generate real-time results based on variants. Over 6-week period during months of April May 2021, we set out assess TaqMan Mutation Panel Genotyping Assay, initially three...